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Strawberry and Banana DNA Extraction

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by

Emmanuella Alawode

on 11 August 2014

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Transcript of Strawberry and Banana DNA Extraction

Strawberry and Banana DNA Extraction
Procedures
Put the strawberry in the bag, squeeze out most of the air and seal the bag. Then crush the strawberry into juice and pulp. Make sure everything has been crushed and there is no chunks left. Next open the bag and add 10 ml of extraction buffer. Seal the bag again and gently mix the strawberry juice with the extraction buffer. Use the gentle tilting back and forth motion while lightly squeezing the bag. Third, set up a filtration system . Wrap paper towel around your finger, then put your paper wrapped finger into the mouth of 15ml tube or 5oz cup. When you remove your finger it should form a well, where the juice can be poured. Fourth, carefully pour the juice through the filter and let it drip for 3-5 min. Throw away the paper towels into the ziplock bag. Fifth, carefully transfer the liquid from the 15ml tube or cup into the clear test tube until the test tube is about a third full. Sixth, slowly add 3ml (3 eyedroppers full) of ice cold alcohol to the test tube. The alcohol will trickle down on the side of the tube before the pooling strawberry extract. You should end up with a red bottom layer and clear bottom layer.
Materials
1 fresh pack of strawberries
bananas
1 ziplock bag
1 15ml centrifuge tube or 5oz paper bathroom cup
1 clear glass or plastic test tube
1 paper towel
10 ml extraction buffer
bamboo skewer or glass stirring rod
Extraction buffer recipe:
450 ml of distilled water
10 g. of table salt
50 ml Dawn dishwashing detergent


Research

Question

Which fruit; banana or strawberry will have the most DNA extracted?

Hypothesis
I think that the strawberry will have the most DNA extracted, because I think it has more DNA cells because of the strawberry seeds.

Data
Trial one;
strawberry had a very little amount of DNA extracted.

Banana had a larger amount compared to the strawberry that was extracted.

Trail Two:

strawberry had a very little amount of DNA extracted.

Banana had a larger amount compared to the strawberry that was extracted.




Trial Three:

Strawberry had a very little amount of DNA extracted.

Banana had a larger amount compared to the strawberry that was extracted.













Variable
Fruits; strawberry and banana.

Banana Section
Banana DNA Extract
Strawberry Section
Strawberry DNA Extract
Conclusion
Procedures 2
Fifth, carefully transfer the liquid from the 15ml tube or cup into the clear test tube until the test tube is about a third full. Sixth, slowly add 3ml (3 eyedroppers full) of ice cold alcohol to the test tube. The alcohol will trickle down on the side of the tube before the pooling strawberry extract. You should end up with a red bottom layer and clear bottom layer. Seventh, after 2-3minutes, a skewer or stirring rod can be inserted into the tube and gently swirled around, this will spool the DNA. The DNA can be stored in a microcentrifuge tube filled some alcohol. Repeat theses steps with the banana.
In conclusion the banana had extracted the most DNA. I was wrong about my hypothesis, because I had said that the strawberry would have more. But instead it was he banana.
Full transcript