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Effects of Echinostoma caproni infection on the lipids in intestinal and non-intestinal organs in the BALB/c mouse

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Jessica Counihan

on 6 May 2010

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Transcript of Effects of Echinostoma caproni infection on the lipids in intestinal and non-intestinal organs in the BALB/c mouse

Introduction Goals Mice Infections using E. Caproni Neutral and Polar Lipid Extraction Neutral Lipids Table Polar Lipids Table Thank you! Each mouse exposed to ~75 cysts Necropsied 3 weeks PI For the neutral lipids study, amount of worms found in mice ranged from 1-42 Average +- SD = 18 +- 16 Organs dissected: anterior, middle (infected area), and posterior portion of the small intestine, cecum, colon, spleen, kidney, and sample of the liver (100-200 mg) Professors Sherma and Fried Professors Kurt, Husic, and Huang Lafayette College Chemistry Department Phospholipid Chromatograms Neutral Lipid Chromatograms Echinosotma caproni life cycle Quantitative Densitometric Analysis Sphingolipid Chromatograms Effects of Echinostoma caproni infection on the neutral and polar lipids of intestinal and non-intestinal organs in the BALB/c mouse as determined by HPTLC-densitometry by Jessica Counihan Senior Honors Thesis in Chemistry
May 2010 References Produce neutral and polar lipid profiles of various organs in the BALB/c mouse Quantify any statistical differences in percent by mass of lipids in the organs of E. caproni infected versus uninfected mice most likely due to the massive amount of organ relative to the mucosa TGs were the primary neutral lipd class in the organs of BALB/c mice, and the colon contained the largest amount of TGs. For polar lipids, the anterior portion of the SI within E. caproni infected mice contained significantly more PE than uninfected mice within the same organ region. future research will need to be done to determine the significance of this difference In contrast to previous papers that used the intestinal mucosa, we did not determine any differences in neutral lipids between E. caproni infected and uninfected mice. Results Nonpolar Lipid Mixture B Polar Lipid Mix No. 1127 Sphingolipid Mix No. 1128 Neutral Lipids Mangold mobile phase HPTLC Analysis Polar Lipids Wagner mobile phase Smith mobile phase All chromatograms were dried, sprayed, and heated Past studies have looked at the lipids in E.caproni infected versus uninfected mice within the intestinal mucosa, serum, and liver. Saric et al. profiled a wide range of metabolites, including lipids, in whole organs from mice infected with E. caproni and uninfected mice. did not qualitatively or quantitatively profile the lipids For the polar lipids study, amount of worms found in mice ranged from 22-40 Average +- SD = 30 +- 8 Homogenize Sample Folch wash Vortex Mix Extract aqueous layer Reconstitute Sample PC was more abundant in the anterior region of the SI than in the middle or posterior regions Liver, kidney, and spleen had higher amounts of PC and PE than the intestinal organs
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