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SWEET

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by

Pierre Gebauer

on 16 November 2014

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Transcript of SWEET

SWEET
Induction
Pathogen
Chigg, Pst
wounding
sucrose export
senescence
induction by different compounds (test)
S11/12 increased resistance
Priming

marker for priming?
co-regulated genes?
What leads to the induction of this transporter?
Impression: earlier senescence in S11/12
sugar exudation
Differences between light and dark period?
cross S11/12 and pgm
changes in PT of pgm (reduced root respiration due to the lack of S11 and 12?
- increased carbohydrate content
- SA induction upon covering
- MA data - induction of defence related genes
function as sucrose exporter
high carbohydrate content of S11/12
- reduced exudation rate during the light period in the double mutant plant
- reduced root respiration?
- increased leaf respiration?

siehe Chong 2014 Vv

- ROS induced?
triple mutants
10 & 15

- resistance compared to S11/12?

amiRNA plants
- resistance compared to S11/12?
- growth PT and RNAi effect?
carbohydrate
content?
Oex constructs increased exudation rate
induction S12 only at the site of infection (vicinity) (vasculature, epidermis and mesophyll cells)
reduced exudation rate upon infection
S11 only slightly induced in vasculature at the infection site

Oex = expression in "all" cells leads to increased exudation rate
transporter expression at the phloem parechyma cells not so important for phloem loading
also expression in companion cells (would lead to leckage out of the SCC and reduced phloem loading)
S13 upregulated in S11/12

Oex plants reduced starch accumulation to Col-0 at the end of the dark period
S11/12 high starch PT - reduced starch mobilisation
Oex higher nocturnal starch mobilisation
siehe Brauner et al. 2014
cytoplasmic available sucrose will be exported
increased export by Oex leads to constitutive sugar signal in the cytosol for lack of sugar
Oex more susceptible?
differentiation between increased sugar availability for the fungus or reduced sugar for defence?
bidirectional (in and out)
UmamiT
AA transport
bidirectional (in and out)
unter endogenen Promotor:
schwierig zu sagen, da eventueller Effekt nicht zugeordnet werden kann
wenn die Pflanzen suszeptibler werden kann es sein, dass vermehrt Zucker für Pathogen zur Verfügung oder verminderte Verfügbarkeit für die Abwehr
erhöhte Toleranz würde für Einfluss auf Abwehrreaktion (Versorgung) sprechen - dies aber wieder schwer zu erklären, dass die Einzelmutante keinen signifikanten Suszeptibilitäts-PT
bei 35S Oex kann der Effekt überhaupt nicht zugeordnet werden
UmamiT
18
siar1-1, Ladwig, 2012
Gln, (Val, Asp, Ile (His)) (Pro in siliques)
constant light conditions - surplus carbohydates and nitrogen limitation
antocyanin accumulation = stress response to nitrogen limitation
induction by Pst 3000 but not by hrp-
hrp- no functional T3SS
no injection of effectors
no induction of S12 (only very weak)
what induces S12 by Pst infection?
would it be HR - than there should also be (or stronger) induction by hrp-


Chigg - induces S12 but not at the stage of appressoria formation
was tested with a mutant defective in PH formation - no induction of S12
somthing to do with penetration / PM/ZW integrity
but no induction by Ec - which also builds up intracellular structures
probably elaborated mechanisms to control host responses?
what is the trigger for induction?
testing different components for induction
- hormones and ...
- infection with HR inducing bacteria
(which one?)
Full transcript