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Makahiya (Mimosa pudica)

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rosario rapsing

on 10 October 2014

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Transcript of Makahiya (Mimosa pudica)

Makahiya
(Mimosa pudica)
Root Extract as an Alternative Ovicide against Dengue Mosquito
(Aedes aesgypti)
Eggs

METHODOLOGY
2.1 Material/ Equipments
RESULTS AND DISCUSSION
SAMPLE SURVEY
• Makahiya Root (20 grams)
Mortar and Pestle
4 Petridish
1.6 Review of Related Literature
INTRODUCTION
3.1 Findings
1. T1:
1 ml with 1 ml of distilled water
4 out of 5 (aedes aegypti) mosquito eggs died.
2. T2:
5 ml with 1 ml of distilled water
5 out of 5 (aedes aegypti) mosquito eggs died.
3. T3:
Distilled water
0 out of 5 (aedes aegypti) mosquito eggs died.
4. T4:
5 ml extract
5 out of 5 (aedes aegypti) mosquito eggs died.

The augmenting number of Dengue cases not only in our country but also to other countries all over the world experiencing the abnormal change in weather is not of recent origin to us. Along with this difficult situation, there are two-winged, dipterous insects that hatch their eggs for a short time, reducing the size of their larvae and become “nervous-biters”. In that case, they are harder to intercept in flight that is why it is more advisable to focus on the places where they lay their eggs.

The researchers conducted this study in order to lessen the proliferation of mosquitoes or prevent the eggs from hatching using the Makahiya Mimosa pudica roots extract as an ovicide. As the experiment was proven to be effective, it is relevant to propagate the plant for mass production and communicate the results to other people. It is also significant to conduct experiments about the other potentialities of the said plant, such as the anti fertility property of its root extract

Mimosa pudica (from Latin: pudica "shy, bashful or shrinking"; also called sensitive plant, sleepy plant and the touch-me-not), is a creeping annual or perennial herb often grown for its curiosity value: the compound leaves fold inward and droop when touched or shaken, to protect them from predators, re-opening minutes later. The species is native to South America and Central America, but is now a pantropical weed. It grows mostly in shady areas, under trees or shrubs.

Mimosa pudica contains the toxic alkaloid mimosine, which has been found to also have antiproliferative and apoptotic effects. The extracts of Mimosa pudica immobilize the filariform larvae of Strongyloides stercoralis in less than one hour.Aqueous extracts of the roots of the plant have shown significant neutralizing effect in
Mosquitoes are one of the most unwanted and harmful pests known to man.
In feeding on blood, some of them transmit extr
emely harmful human and livestock diseases, such as malaria, yellow fever, filariasis and dengue. Some authorities argue accordingly that mosquitoes are the most dangerous animals on Earth.

Throughout the history, many plants have been used as an alternative insecticide, ovicide, and repellents. Ovicides are any substances that are designed to kill eggs, especially the eggs of insects.

The Makahiya plant is widely distributed in the Philippines in open, moist, waste places, open grasslands and open thickets, at low and medium altitudes in settled areas.
1.1 Background of the Study
1.2 Statement of the Problem
General Objectives
 To produce alternative ovicide out of a natural ingredient.
 To find out possible effect of Makahiya extract.

Specific Objectives
 Does alternative Makahiya extract is effective than chemical insecticides?
 Is there a significant effect using Makahiya using this kind of ovicide?

1.3 Hypothesis of the Study
HO: There will be no significant difference between the samples as the time increases.
HO: There will be no significant difference between the four treatments.
HO: There will be no interaction effect between time and four treatments.
H1: There will be a significant difference between the samples as the time increases.
H1: There will be a significant difference between the four treatments.
H1: There will be an interaction effect between time and four treatments.
1.4 Significance of the Study
This study aims to determine if the Makahiya
(mimusa indica)
root extract could be an alternative ovicide against
(aedes aegypti)
mosquito eggs.
Specifically, the study sought to answer the following question:
1. Is Makahiya
(mimusa indica)
root extract responsible for the activity against
(aedes aegypti)
mosquito eggs?
2. Is there a difference between the samples as the time increases in
(aedes aegypti)
mosquito eggs?
3. Is there a significant difference between the different amounts of extract being applied in
(aedes aegypti)
mosquito eggs?
1.5 Scope and Limitation
This research is limited only on the effect of Makahiya extract. This is to determine the effect of ovicide on the death rates eggs of mosquitoes.
the lethality of the venom of the monocled cobra (Naja Kaouthia). It appears to inhibit the myotoxicity and enzyme activity of cobra venom.
 Makahiya Root (20 grams)
 Distilled Water (20 ml)
 4 Petridish
 Strainer
 Beaker
 Weighing Scale
 Mortar and pestle
 Measuring Pipette
 Graduated Cylinder
NAME:
SCHOOL:
1. Four beakers will be used. Each beakers will contain 5 eggs and will be labeled (T1, T2, T3 and T4)
2. Each beaker will add extract with different level of concentrations:
T1: 1 ml with 1 ml of distilled water
T2: 5 ml with 1 ml of distilled water
T3: Distilled water
T4: 5 ml extract
3. The mortality rate of eggs will be observed after 10 minutes, 20 minutes, and 30 minutes.
1. The materials needed for the experiment will be collected.
2. 20 g of makahiya root will be pounded with the use of mortar and pestle. 10 ml distilled water will be added.
3. The product will be squeezed and filtered using the strainer. The extract will be obtained.
PREPARATION
2.2 General Procedure
APPLICATION
1. Does the Makahiya extract has a good fragrance?
2. Does the Makahiya extract kill the eggs of mosquitoes?
3. Does it irritate your skin?
4. Does it irritate the eyes?
5. If it became a product, Are you going to buy it?
CRITERIA
YES NO
BEAKER
Water and Graduated Cylinder
Weighing Scale
PREPARATION
T1: 1 ml with 1 ml of distilled water
T2: 5 ml with 1 ml of distilled water
T3: Distilled water
T4: 5 ml extract

CONCLUSION
From the study conducted, the researchers would like to recommend this study to the students who would pursue the “Makahiya (Mimosa pudica) Root Extract as an Alternative Ovicide against Dengue Mosquito (Aedes aegypti) Eggs” as mosquito killer to find other insects in which the solution is applicable and conduct further study on the utilization of the solutions.
RECOMMENDATION
Based on the findings, the following conclusions were drawn:
The Makahiya (mimusa indica) root extract is responsible for the activity against (aedes aegypti) mosquito eggs,
There is a difference between the samples as the time increases in (aedes aegypti) mosquito eggs.
There is a significant difference between the different amounts of extract being applied in (aedes aegypti) mosquito eggs.
Nodado, Demi Jam
Rapsing, Rosario D.
Ebidag, Jennylyn
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