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Copy of Paper Chromatography Lab

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by

Liz Holland

on 13 August 2014

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Transcript of Copy of Paper Chromatography Lab

Paper Chromatography
What is paper chromatography?
Chromatography Setup
Key Parts of Chromatography
Rf Value (Retention Factor)
The separation of a mixture using intermolecular forces.
Materials: chromatography paper, component mixture (what you are separating), solvent, container with a lid, pencil, ruler
Component Mixture: What you are separating

Stationary Phase: The chromatography paper

Mobile Phase: The solvent
= distance traveled by component (mm)/
distance traveled by solvent
What does this mean?
The higher the Rf value, the further it traveled = stronger IMF with the mobile phase.
The smaller the Rf value, the less it traveled = stronger IMF with the stationary phase.
How do they relate?
There are IMF between the component and the stationary phase
There are IMF between the component and the mobile phase
Your Mission
Design an experiment
Objective: To separate the dye molecules: Blue , Red and Yellow from black ink using paper chromatography
Your solvents:
Ethanol
Distilled water
Acetone
Requirements:
Pre-Lab Question:
Predict which would be the best solvent to separate the mixture.
Method:
Write a step by step procedure to separate the ink using the solvents provided.
Data:
Record Rf values for each solvent & color separated on a data table.
Lab Report
Analysis:
Explain how intermolecular forces (like dissolves like) and the molecular structure of the molecules being separated determine the ideal solvent for chromatographic separation. Cite specific evidence from your data.
Conclusion:
Was your original prediction correct? If so, what made you decide on that particular solvent. If not, why wasn't that the best solvent?
Another type is called Column Chromatography
How to Calculate Rf
Full transcript