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The Analysis of Vitamin D

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Kelsey Willis

on 24 April 2013

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Transcript of The Analysis of Vitamin D

The Analysis of Vitamin D Dr. J. D. Patterson

Dr. Bradshaw

Ouachita Baptist University

NASA/ASGC Acknowledgments why the big Deal? D assists the immune
in fighting off bacteria and viruses helps the body
absorb calcium
to promote healthy bones aids in muscle movement aids in the transfer of messages
by nerves How do we acquire Using HPLC by Kelsey Willis vitamin D? D photo courtesy of pensteindermatology.com photo courtesy of yinyangastrologer.com photo courtesy of inquisitr.com the body must be directly exposed to the sun to acquire vitamin D from the sun Supplements are found in two different forms D2 (ergocalciferol)
D3 (cholecalciferol) Very few foods naturally have vitamin D. Fortified foods provide most of the vitamin D in American diets. In order to produce the body's required vitamin D levels the skin should be exposed to the sun ten to fifteen minutes three times a week. clouds, shade, and having dark-colored skin reduces the amount of vitamin D created in the skin. How much is too much? How much is just right? Life Stage Recommended Amount
Birth to 12 months 400 IU
Children 1–13 years 600 IU
Teens 14–18 years 600 IU
Adults 19–70 years 600 IU
Adults 71 years and older 800 IU
Pregnant and breastfeeding women 600 IU Almost no one in the United States has levels that are too high.
However, levels above 125 nmol/L (50 ng/mL) are most likely too high.
An excess of Vitamin D can cause confusion, disorientation, problems with heart rhythm, and damage to the kidneys.
The body limits the amount of Vitamin D in produces from sun exposure.
Vitamin D toxicity almost always occurs from overuse of supplements. picture courtesy of topnew.com How much is too much? Levels below 30 nmol/L (12 ng/mL) are considered too low for overall health
Three-quarters of U.S. teens and adults are deficient in vitamin D
50% of the global population is at risk How much is just right? photo courtesy of freshhealthyvending.com What about being D-ficient? D who is at risk? breastfed infants
older adults
people with dark skin
obese individuals image courtesy of manaphy.edu.glogster.com photo courtesy of breastfeeding.com photo courtesy of writerzblockblog.wordpress.com photo courtesy of topnews.ae What if you are D-ficient?? in children in adults osteomalacia- weak bones rickets- a disease
characterized by a
failure of bone tissue to properly mineralize, resulting in bone and skeletal deformities HPLC high performance liquid chromatography Results Synthesis of Pip-OH and Pip-Et-OH
as Possible
Photodynamic Therapy (PDT) Agents. Part Two: What is Photodynamic Therapy? It is a treatment that uses a drug called a photosensitizer or photosensitizing agent, and a particular type of light to treat cancer.
When these drugs are exposed to a specific wavelength of light, they produce a form of oxygen that kills nearby cells.
Each photsensitizer is activated by light of a specific wavelength D2 100 % Stock- 1,000 ppm D2 10% stock D2 2% Stock D2 1% Stock Nova-Pak C18 Mix Phenomenex MAX RP Mix Luna C18 (2) 1% MQ 99% MeOH 25% MQ 75% MeOH- 20 min 25% MQ 75% MeOH- 30 min 5% MQ 95% MeOH Gradient Column at 40 C flow of .5 mL/min 15 ppm rat serum Further results We ran a sample 3x in a row and found that there is on average a 3% injection error for the HPLC machine.
We tested different serum extraction methods to see which gave the best results.
We tested whether making the HPLC samples a day ahead of time decreased accuracy of our results.
We are currently testing whether using a centrifuge in the fridge will decrease our % error
In the future we plan on testing plastic centrifuge tubes vs glass to see if the plastic is absorbing the Vitamin D. Recommended Dietary Allowances (RDA) set by the Food and Nutrition Board (FNB)at the Institute of Medicine of the National Academies These two forms have traditionally been considered equivalent.
Most steps involved in the metabolism and actions are identical.
However, while nutritional doses are equivalent, high doses of D2 are less potent.
Dr. Bradshaw
Office of Dietary Supplements National Institutes of Healt
ScientificAmerican.com Sources What is Pip-OH and Pip-Et-OH Modified porphyrins.
Organic heterocyclic macrocycles.
Composed of four modified pyrrole subunits.
We modify the side chains. For Pip-Et-OH For Pip-OH area under peak- 24392
calculated ppm using the line of best fit for the graph- 0.8516 ppm
percent difference from the known value of .75 ppm- 12.689% Unknown of a known value at .75 ppm
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