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Capstone Presentation 2

Precise temporal regulation of roughest is required for correct salivary gland autophagic cell death in drosophila.

Zach Goldstein

on 18 February 2010

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Transcript of Capstone Presentation 2

Precise Temporal Regulation of roughest is Required for Correct Salivary Gland Autophagic Cell Death in Drosophila. Cell Death Apoptosis Necrosis Autophagy Drosophila
Development Life Cycle Programmed Cell Death http://www.seop.yale.edu/imgs/dresophila.jpg Claudio R. Simon, Livia M.R. Moda, Shirlei Octacilio-Silva, Lucas Anhezini, Luciana C.H. Machado-Gitai b, Ricardo Guelerman P. Ramos Roughest (rst) Orthologs 'Death Casette'
important genes
roughest (rst) Kin of IRRE-like protein 3
KIRREL 2 http://www.siumed.edu/~dking2/crr/images/corp5.jpg irregular chiasmC-roughest
protein product
seye roughness Bao, S., Cagan, R.(2005) Preferential adhesion mediated by Hibris and Roughest regulates morphogenesis and patterning in the Drosophila eye. Dev. Cell 8:925–935. hours APF: third instar larva 10 14 12 head eversion ecdysone 'pulse'
salivary gland autophagy salivary gland cells contain vacuoles containing organelles - hallmark of autophagy http://www.vet.uga.edu/vpp/clerk/collins/fig03.jpg http://www.lib.uiowa.edu/haRDIN/MD/pictures22/cdc/PHIL_1125_lores.jpg Model and Methods What role does roughest play in salivary gland development during Drosophila pupae formation? Mutants
rstD Methods
scoring salivary glands
Salivary Gland Persistence Effect of rst misexpression on autophagy Structure of rstD persistent salivary glands rst expression in wt during
late third instar larva wt, heat shocked wt transgenic rst rstD rstD rstD rstD rstD wt, 12 h APF wt, 12 h APF rst expression in rstD mutants rstD wt Conclusions novel role for roughest
depends on precise downregulation of rst - antagonist of death machinery
first description of rst in this organ between pupariation, head eversion, and histolysis
data not shown - indirect antagonist Future experiments specify role
involvement in cytoskeletal changes
ecdysone repression
functional diversity - different mechanisms? wt 3h APF
Full transcript