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The Antibacterial Property of Lakatan Banana (Musa acuminata)

Research
by

Joseph Gil

on 10 March 2013

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Transcript of The Antibacterial Property of Lakatan Banana (Musa acuminata)

Timeline 2013 2009 2010 2011 2012 Group 2 0 + - = 9 8 7 1 2 3 4 5 6 c THE ANTIBACTERIAL PROPERTY OF (Musa acuminata) LAKATAN BANANA FLESH EXTRACT Hypothesis Conceptual Framework Research LAKATAN? Null Hypothesis: The Lakatan Banana (Musa acuminata) flesh extract does not have an anti-bacterial property that can inhibit the growth of common skin bacteria.
Alternative Hypothesis: Alternative Hypothesis: The Lakatan Banana (Musa acuminata) flesh extract has an anti-bacterial property that can inhibit the growth of common skin bacteria. Introduction
Does Lakatan(Musa acuminata)I banana flesh extract have an antibacterial property?

Specific questions
What specific type of bacteria will Lakatan banana flesh extract affect on the experiment?
What methods will the researchers use to know if there is an antibacterial property in Lakatan banana flesh extract
If there is an antibacterial property in Lakatan banana flesh extract, how can you use it? Specific Questions Background of the Study The aim for this study is to PROVE that Lakatan flesh extract has an Antibacterial property against common skin bacteria. Before the researchers concluded with this research proposal, a survey was conducted around a community The results of the survey was amassed on contacted disease. The group envisioned bacteria, and in finding a source of alternative antibacterial substances that is both cheap and accessible such as the, Lakatan. Bacteria which is commonly found on the skin is the foremost intention, of the group to prevent in infecting open wounds. INPUT •Lakatan Banana Flesh extract
• Antibacterial property of Lakatan banana PROCESS •Agar disk diffusion
•Process the flesh extract Output • Product that the researchers will create to use the antibacterial property of Lakatan banana
•Data gathered from the test. Statement of the Problem Does Lakatan banana flesh extract have an antibacterial property on common skin bacteria? This study aims to prove that Lakatan banana flesh extract has an antibacterial property. This will benefit all the people who are allergic to other chemical products. This study shows that there are many kinds of natural resources that have an antibacterial property, not yet discovered. If it proves to have such properties, then it shall be used and encouraged to be used and further tested for more knowledge of the subject and if success will happen, this study will continue on its research an tested until it is proven. Significance of the Study ASSUMPTIONS The problem the antibacterial property of the Lakatan banana can affect a specific type/s of bacteria that can be tested through the methods that the research group will do. The researchers can make a liniment, a lotion, oil, or a supplement for this proposal. Definition of Terms Agar Disk Diffusion- The agar diffusion test, or the Kirby-Bauer disk-diffusion method, is a means of measuring the effect of an antimicrobial agent against bacteria grown in culture.

Kirby-Bauer antibiotic testing (KB testing or disk diffusion antibiotic sensitivity testing) - is a test which uses antibiotic -impregnated wafers to test whether particular bacteria are susceptible to specific antibiotics An Antibacterial is a compound or substance that kills or slows down the growth of bacteria. What is an AntiBacterial? Agar What? In microbiology, Agar is used to provide a solid surface containing medium for the growth of bacteria and fungi. Microbial growth does not destroy the gel structure because most microorganisms are unable to digest agar. LAKATAN BANANA(Musa acuminata) is a species of wild banana native to Southeast Asia. It is the progenitor/ancestor of modern edible bananas, along with Musa Balbisiana and it is one of the earliest domesticated plants. What is Bacteria? Singular for bacterium, are a large domain of prokaryotic microorganisms. Also are microscopic organisms whose single cells have neither a membrane –enclosed nucleus nor other membrane-enclosed organelles like mitochondria and chloroplasts. There are typically 40 million bacterial cells in a gram of soil and a million bacterial cells in a milliliter of fresh water; in all, there are approximately five nonillion (5×1030) bacteria on Earth.  Forming a biomass that exceeds that of all plants and animals. With large numbers of bacteria on the skin and as gut flora, but the bacteria in the human body are aimed to be harmless because of our immune system. Scope and Limitations This study “The anti-bacterial property of (Musa acuminata) flesh extract in common skin bacteria” is focused on the Lakatan banana that the researchers will experiment on, to know if there is an antibacterial property on the said fruit species. This study will cover the properties of the banana and its effects to living organisms such as humans. The researchers will only test the Lakatan (Musa acuminata) and no other fruit species or any other banana subspecies. Antibacterial substances are sought out for medicinal purposes for external/internal body treatment, medicine enough to satisfy a given population affected by certain diseases. The researchers thought of a problem on the antibacterial property of the local plant, Lakatan (Musa acuminata) a banana subspecies that can be seen in the Philippines. The researchers have thought of the problem so that to increase the variety of sources for antibacterial substances. The Lakatan, being a local plant will make it easier for a big scale exploitation of the resource. The antibacterial properties of the substance may generate interest in further studies and production. Related Studies Evaluation of the antimicrobial properties of unripe banana (Musa sapientum L.), lemon grass(Cymbopogon citratus S.) and turmeric (Curcuma longaL.) on pathogens.

Studies on the antibacterial activity of root extracts of Carica papaya L

Antibacterial and Antioxidative Activities of the Various Solvent Extracts of Banana (Musa paradisiaca cv. Mysore) inflorescence

Antimicrobial Properties of Garlic Oil against Human Enteric Bacteria Methodology The researchers will make use of the agar disk diffusion method. The researchers will be discreet in handling the bacteria. There is a need to process the Lakatan flesh extract with a blender to make the substance liquid like. The researchers will put the extract into a test tube and will soak the filter paper disks using tweezers.
*The Agar Disk Diffusion Method
*Measuring of the Zone of Inhibition
*Making of the Product
*Tabulation of Data and Conclusions
the Staphylococcus aureus and the Staphylococcous epidermidis shall be classified and labeled as either susceptible or resistant to the Lakatan banana extract. Research Design The researchers used single group design because it is more appropriate for their study. Staphylococcous epidermidis Staphylococcus aureus Zone of Inhibition in (mm) Lakatan Banana Flesh Extract Special Acknowledgement to Michael Abrigos, Ismael Soliman and Joseph Gil and to... FIN. the Staphylococcus aureus is one of the five most common causes of infections after injury or surgery. This bacterium is normally present on the skin, nose and pharynx of many humans and animals. It causes acute infections - a short-term infection. the Staphylococcus epidermidis is a leading nosocomial pathogen. It causes chronic infections – a long-term infection. Staphylococcus epidermidis colonizes the epithelial surfaces of every human being. This study is unique because of the different variables used, compared to the other studies, which involved other fruits. Lakatan, our own local banana species may have an anti-bacterial property, for it is in the same species as the bananas used in the related studies which has confirmed various anti-bacterial properties. Justification of the Present Study
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