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Biology 481: Antibiotic Resistance Lab

Thursday 8, 2011

Arya Mehr

on 23 February 2012

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Transcript of Biology 481: Antibiotic Resistance Lab

Analysis and Discussion
Antibiotic Resistance Lab
Data & Results
Special Thanks!
-Ben Kerr
-481 TA's
* ≥75%: R (resistant); ≤10%: S (sensitive); in between: M (mixed).
Table of the fitnesses of experimental replicates along with controls relative to the sensitive BK27 ancestor
Lexie Miller, Kameron Firouzi, Brittany Gjestrum, Arya Mehr, and Sara Kunishige
What the Flux
Step 1: Isolate Resistant Bacteria
Expose bacteria to Rifampicin and select colonies that survive
Step 2: Evolve Resistant and Sensitive Strain 26 Days
Freeze a resistant strain and sensitive strain at day 2.
Evolve resistant and sensitive strains 26 days, transfering every 24 hours
Step 3: Compete All Strains with "Ancestor" BK27
Strains Competing:
Resistant Day 2 vs. BK27
Resistant Day 26 vs. BK27
Sensitive Day 2 vs. BK27
Sensitive Day 26 vs. BK27
Plate each competition on LB agar to quantify competition results (BK27 is red and BK26 is pink).
Step 4: Plate Day 26 Resistance Strain on Rifampicin
Determine if strain is still resistant after 26 days
Table of usable colony counts on LB and Rif plates with corresponding desigation of suceptibility to Rifampicin after 26 days of evolution
Our results from the Antibiotic Resistant Experiment concluded that...

Our data rejected both null hypotheses

Our data showed that the fitness of BK26, relative to the ancestor increased and that
the bacteria were resistant to
rifampicin at day 26

Results showed compensation
likely occured
MRSA: Methicillin-resistant Staph aurures
MDR TB: Multi-Drug Resistant Tuberculosis
Two Billion People
(1/3 of the Worlds Population)

Two Percent of the Population has Multi-Drug Resistant Tuberculosis
What the Flux
Fig. 6

Note: Error bars indicate standard deviation
Fig 5.
Antibiotic Resistance

Bacteria quickly confer resistance to antibiotics

Resistance often comes at a cost to bacteria

These resistant strains tend to have a lower fitness in antibiotic free environments

Would these bacteria lose their resistance when the antibiotics are removed?

Compensation vs Reversion
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 7 Table showing two-tailed T-tests and their interpretations
Fig. 8
MRSA people of all ages, genders, and social standings

MRSA related pneumonia and blood infections are coupled with a high death rate
Decreasing time between dosages
Drug cocktails decrease evolution of resistance
Full transcript