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Potato and Hydrogen Peroxide Enzyme Lab

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Brittany Gotts

on 27 February 2014

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Transcript of Potato and Hydrogen Peroxide Enzyme Lab

Potato and Hydrogen Peroxide Enzyme Lab
Experimental Variables:
Question:
How do environmental factors affect enzyme function?


Applying environmental factors such as heating or cooling enzymes, will change the process and the rate at which they function.


Hypothesis:
If the peroxidase enzyme is reacting with potatoes at temperatures extremely high, the reaction will become denatured causing no reaction to occur.

Procedure:
1.Take a raw potato and cut it up into at least 5 1cm x 1cm x 1cm cubes.
2.Label Beakers and Graduated Cylinders “very cold”, “cold”, “room”,”warm”, and “very hot.”
3.Fill each graduated cylinder with 3mL of hydrogen peroxide.
4.Pour 6cm of cold water into beaker labeled “very cold” and put in freezer until water is at 5°C (check with thermometer.)
5.Then remove from freezer and place graduated cylinder “very cold” in with a potato cube. Allow temperatures to stable for 5 minutes.
6.Put thermometer in the graduated cylinder and once peroxide is 5°C, add the same potato cube to that cylinder and remove thermometer
7.Observe the reaction. Foam (bubbles released from hydrogen peroxide which indicate a chemical reaction) should form at the top of the liquid as a result of the hydrogen peroxide mixing with the potato.
8.Record the height of the bubbles after 30 seconds, 1 minute, and 2 minutes using the stopwatch.


Procedure: (2)
9.Pour 6cm of cold water into beaker labeled “cold” and add two ice cubes.
10.Put thermometer in water and wait for it to reach 10°C.
11.Place Graduated cylinder labeled “cold” into the beaker with one of the potato cubes. Allow temperatures to stabilize for 5 minutes.
12.Put thermometer in graduated cylinder and once peroxide is 10°C also, add the same potato cube to that cylinder and remove thermometer.
13.Repeat steps 7&8.

Materials:
1 Potato cut into 5 small pieces
6 Beakers
6 Graduated Cylinders
Ruler
Hydrogen Peroxide
Water
Pot
Heat source (stove)
Ice
Freezer
Thermometer (celsius)
Stopwatch/Timer

Independent variable:
Time & Temperature potato and peroxide
Dependent variable:
Height of bubbles
Procedure: (3)
14.Pour 6cm of room temperature water into beaker labeled “room”.
15.Put thermometer into beaker and let water sit until its 20°C.
16.Place graduated cylinder labeled “room” into beaker with a potato cube. Allow temperatures to stabilize for 5 minutes.
17.Put thermometer in graduated cylinder and once peroxide is 20°C, add the potato to that cylinder and remove thermometer.
18.Repeat steps 7&8.

Procedure: (4)
Procedure: (5)
24.Fill pot half way with water and allow water to boil. Once it is boiling, fill beaker labeled “very hot” with 6cm of water.
25.Let the water cool to 80°C using thermometer.
26.Once cooled, place graduated cylinder labeled “very hot” into beaker with a potato cube. Allow temperatures to stabilize for 5 minutes.
27.Put thermometer in graduated cylinder and once peroxide is 80°C, add the potato cube and remover thermometer.
28.Repeat steps 7&8.



Hannah Mahoney & Brittany Gotts

The control experiment of hydrogen peroxide and water.
Control Procedure
1. Label one beaker and graduated cylinder “control”
2. Pour 6 cm of water into beaker.
3. Add 3mL of hydrogen peroxide.
4. Notice no change in the water which proves no reaction has occurred.
This is the hydrogen peroxide reacting with the potato
GRAPH
Conclusion
This experiment proved our hypothesis to be correct because once the temperature of the potato and hydrogen peroxide was greater than 55°C, the enzyme was denatured and could no longer carry out its function. It proved that environmental factors to alter enzyme function and in order to thrive the conditions must be perfect.
Potato and Hydrogen Peroxide Experiment
Peroxidase
Introduction
Peroxidase: an enzyme which breaks down oxidation in a substance by hydrogen peroxide
Hydrogen Peroxide: a clear liquid with oxidizing properties
Potatoes, Labeled Beakers and Hydrogen Peroxide
Visuals of Experiment
Data Table
19.Fill pot half way with water and keep it on stove until temperature is 50°C using thermometer.
20.Pour 6cm of this water into beaker labeled “warm”.
21.Place graduated cylinder labeled “warm” into beaker with a potato cube. Allow temperatures to stabilize for 5 minutes.
22.Put thermometer in graduated cylinder and once peroxide is 50°C, add the potato to that cylinder and remove thermometer.
23.Repeat steps 7&8.

Discussion

Does the lab support the hypothesis?
Yes, the lab proved the hypothesis to be true.
Possible Sources of Error:
not pouring correct amounts of hydrogen peroxide
not measuring bubble heights correctly
Ways Results could have been improved:
testing bubble heights after longer time periods
testing more temperatures
How does this relate to class?
enzymes are an important topic in biology and this hands-on experiment showed they are part of our everyday lives
Citations:
The End
"Science Fair Projects - How Temperature Affects the Peroxidase Enzyme." Science Fair Projects - How Temperature Affects the Peroxidase Enzyme. N.p., n.d. Web. 25 Feb. 2014.
"Peroxidase Enzymes." Sigma-Aldrich. N.p., n.d. Web. 24 Feb. 2014.
Full transcript