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Electric Birefringence

Abhiram Vyas & Pranil Khatri
by

abhiram vyas

on 1 October 2013

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Transcript of Electric Birefringence

Birefringence
Electric Birefringence
Birefringence, or double refraction, is the decomposition of a ray of light into two rays when it passes through anisotropic materials,
aniostropic materials ie) crystals of calcite or boron nitride
Anisotropy :
the property of being directionally dependent
ie) Wood
Isotropy:
identical properties in all directions
Refraction
Double
Electric Birefringence
Electric fields can cause orientation of macromolecules or colloidal particles if they have a permanent dipole moment, therefore making the solution optically anisotropic. It is also known as Kerr Effect.

B=Kerr Constant
lambda is the wavelength of the light
delta n is the induced birefringence
E is the electric field.
HOW DO WE...
DETERMINE THE SIZE OF DNA?
DETECT CONSTRICTION
OF ACTIN FIBRES?
evaluate proprties of molecules?
The effect was first described
by the Danish scientist
in 1669, who saw it in calcite
Rasmus
Bartholin
Where,
the
KERR
EFFECT
Positive and Negative Birefringence
Negative Birefringence is when the velocity of the ordinary is less than the velocity of the extraordinary.
Positive and Negative Birefringence
Positive birefringence is when the velocity of the ordinary wave is faster than the velocity of the extraordinary wave
Can be used with various other techniques to find their physical properties of macromolecules.
orientation of actin in muscles
Has medical purposes;
Can be used to identify if a person has gout.
can be used in assessment of gloucoma.
Application of Electric Birefringence
Gout
-accumulation of uric acid in joints
-inflammatory arthritis—a red, tender, hot, swollen joint.
-Excess uric acid in blood crystalized and deposited in joints
-diagnosed from negative birefringence ( synovial fluid sample contains monosodium urate crystals, which have negative birefrifringence
Actin
-involved in muscle contraction
-G Actin : monomer
-F Actin: polymer
Gloucoma
The End
Abhiram Vyas
Pranil Khatri
Full transcript