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Copy of Copy of MICROBIOLOGY SAFETY & RULES

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alberim kurtishi

on 10 September 2015

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Transcript of Copy of Copy of MICROBIOLOGY SAFETY & RULES

Fall 2015: Welcome to Microbiology
ORIENTATION: MICRO LAB SAFETY & RULES
lAB 1: MICROSCOPY
iMPORTANCE OF HANDWASHING


Biohazard Materials: Contaminated gloves, Paper towel, Petri Dishes (plates)
GOES IN BIOHAZARD
RED PLASTIC BAG
ON TABLE
(Solid Biohazard)
Broken glass: Broken pipettes, Broken test tubes, broken slides & cover slides
GOES IN WHITE/
GREEN

BROKEN GLASS BOX
Liquids:
Red Trash container
Next to sink (Liquid Biohazard)
Sharps: pippettes, Slides & Cover slides (Anything sharp but not broken)
GOES IN BIOHAZARD
Red platic bag in glass box
Bright Field Microscope
Dark Field Microscope
Phase Contrast Microscope
Fluorescence Microscope
Objective Lens: *Low Power* high dry * Oil Immersion
10X 40X 100X


Eyepiece (Ocular Lens):


* Eye-Piece Micrometer or Reticle
* Stage Micrometer
Microscopic Measurements Using a Ocular and Stage Micrometer
Dr. Ignaz Phillip Semmelweis.
1. An Instructor/TA should be present be
fore
you

can enter the lab

2. Wear Lab coat at all times

3. Dump Materials in right place (discussed later)

4. The three day rule ( discussed later)

5. provide storm card to use
certain equipments
MICRO LAB SAFETY:
RULES & REGULATIONS
WEAR GLASSES INSTEAD OF CONTACTS FOR LAB

DO NOT INGEST ANYTHING IN THE LAB

DO NOT EAT IN THE LAB

DO NOT PUT ON MAKE UP IN LAB

READ AND FOLLOW INSTRUCTIONS
FOR YOUR OWN SAFETY
Total Power of Magnification =
the magnification power of the objective
X
magnification power of the eye piece

IMPORTANCE OF HANDWASH
The Light Microscope
Parts of the Microscope
Three Day Rule
RIGHT PLACE TO THROW MATERIALS AFTER USE
6. No extra time for staining

7. All equipments./ materials should be put back at
the right place

8. Accidentally spilled bacteria culture/ stains should
be quickly cleaned with disinfectants or alcohol (stains)
MICRO LAB SAFETY:
RULES & REGULATIONS (Conti.)
The History of Microscope
Anton Van Leeuwenhoek (1632 -1723)
WHAT WILL BE COVERED IN TODAY'S CLASS

Orientation
Syllabus
Laboratory safety and rules

LAB
Microscopy: types, parts & CALIBRATION

hANDWASHING: importance!

simple experiments




Some results will have to be viewed the next 24- 48 hrs.
However you have 3 days to see the results in case you are unable to show up with Proper excuse.
Will be stored in Either in the Incubator or Refrigerator
Exercise (Who can spot the incubator and what is an incubator)

An incubator is a device used to grow and maintain microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity and other conditions
Dark-Field Microscope
Forms Bright Object against dark background
Used for extremely small microbes not visible on bright field
Phase Contrast Miscrosope
enhances contrasts of transparent and colorless objects by influencing the optical path of light.

Fluorescence Microscope
to image specific features of small specimens such as microbes. It is also used to visually enhance 3-D features at small scales

used to stain specific cellular structures and identify cellular locations and interaction of specific molecules within the cell

Bright Field Light Microscope
Forms dark Image against bright background
Calculations
Examples
The Stage
The Sub-stage Condenser

The Iris Diaphragam
Calibration Formula For 1 division of Ocular micrometer or reticle:
Mark at Stage Micrometer X 10um
Mark at Ocular Micrometer/Reticle

=20 x 10um = 6.67um
30



The End of Today's Lecture

Finish up Simple Petri dish Experiments for
handwashing

Quiz Next Week: Covers everything on lecture and Notes you Put down
Total Possible Points: 12 Points

Email with questions:
Kurtisha@stjohns.edu

Thank you.

http://zeiss-campus.magnet.fsu.edu/tutorials/basics/oilimmersionrefractiveindex/index.html

Revolving Nosepiece
Working Distance and Parfocal Length
The Working distance
Defines the maximum distance
at which an objective is able to focus
The Parfocal Distance: Distance from the shoulder of the objective to the focal point.

Most Bright microscopes are parfocal
Full transcript