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DNA - a Presentation

individual presentation for Biology 101
by

Angela Stout

on 23 October 2012

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Transcript of DNA - a Presentation

presentation by Angela Stout
2012FA-BIOL-100-002W: 12FA - BIOL-100 - 002W - Concepts of Biology DNA Technology in Forensics
Genetic Engineering
Genetically Modified Organism
(GMO) Plasmids
Polymerase chain reaction
(PCR) Gene Mapping The alteration and recombination of genetic material by technological means, resulting in transgenic organisms a small circle of DNA that replicates itself independently of chromosomal DNA, especially in the cells of bacteria. Plasmids often contain genes for drug resistance and are used in genetic engineering, since they can be transmitted between bacteria of the same and different species Enables researchers to produce millions of copies of a specific DNA sequence in approximately two hours. This automated process bypasses the need to use bacteria for amplifying DNA. Definitions:
Recombinant DNA
Transgenic This describes the technique of transferring genetic material from one organism into the DNA of another or an animal or plant from a different species using the techniques of genetic modification DNA extracted from two or more different sources such as genes from different organisms and joined together to form a single molecule or fragment Genomic Library a collection of clones made from a set of randomly generated overlapping DNA fragments, representing the entire genome of an organism. Short Tandem Repeats STRs are short sequences of DNA, normally of length 2-5 base pairs, that are repeated numerous times in a
head-tail manner BioBytes:forensics and Biotechnology
DNA Center. http://dnalc.org/resources/animations/pcr.html
Gel electrophoresis http://www.ehow.com/about_5608359_gel-electrophoresis_.htmlGene mapping http://www.wisegeek.com/what-is-gene-mapping.htm
GMO http://www.bt.ucsd.edu/gmo.html
RFLP http://www.bio.davidson.edu/COURSES/genomics/method/RFLP.html
Sensabaugh http://library-resources.cqu.edu.au/JFS/PDF/vol_31/iss_2/JFS312860393.pdf
STR http://www.biology.arizona.edu/human_bio/activities/blackett2/str_description.html RFLP
Restriction Fragment
Length Polymorphism RFLP (often pronounced "rif lip", as if it were a word) is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. Gel Electrophoresis A method of separating, identifying and characterizing mixtures of proteins or nucleic acids
used in research. Hospitals use it to identify unusual proteins or DNA patterns to diagnose diseases, genetic defects and genetic relationships. Dr David Targen “Did you know that only 1 ten of a percent of DNA differs from person to person? But these small differences are making big advancements in the criminal justice system possible. I am talking about forensic DNA.(youtube) Organisms created by the alteration and recombination of genetic material by technological means. Genetic mapping refers to the use of linkage analysis to determine how two genes on a chromosome relate in their positions. Physical mapping, the other type of gene mapping, locates genes by their absolute positions on a chromosome using any technique available. Genetic Probe (Gene Probe)
A fragment of DNA or RNA marked by a chemical or radioactive substance that will bind to a given gene, used as a tag in order to identify or isolate that gene Gene Therapy Gene therapy is an experimental technique that uses genes to treat or prevent disease. In the future, this technique may allow doctors to treat a disorder by inserting a gene into a patient’s cells instead of using drugs or surgery. http://www.google.com/imgres?start=26&num=10&hl=en&biw=1525&bih=635&tbm=isch&tbnid=ypIYhpmJfJI2DM:&imgrefurl=http://www.drugdevelopment-technology.com/contractors/synthesis/dna-technology/dna-technology4.html&docid=1i30HlNbqWnZAM&imgurl=http://www.drugdevelopment-technology.com/contractor_images/dna-technology/4--dna-technology.jpg&w=400&h=300&ei=2siEUNPcJ6PniwLsmIDAAg&zoom=1&iact=rc&dur=343&sig=117709133158191246935&page=2&tbnh=131&tbnw=158&ndsp=32&ved=1t:429,r:23,s:20,i:73&tx=65&ty=51 http://www.google.com/imgres?num=10&hl=en&biw=1525&bih=635&tbm=isch&tbnid=mu-uXhrraHdHLM:&imgrefurl=http://algaeforbiofuels.com/genetic-engineering-brief-overview/&docid=4Id7FGnygN5zvM&imgurl=http://algaeforbiofuels.com/wp-content/uploads/2011/09/genetic-engineering1.jpg&w=380&h=345&ei=XsmEUPrWIq60igL4nICYDQ&zoom=1&iact=rc&dur=420&sig=117709133158191246935&sqi=2&page=1&tbnh=145&tbnw=155&start=0&ndsp=23&ved=1t:429,r:8,s:0,i:158&tx=45&ty=63 http://www.google.com/imgres?hl=en&biw=1525&bih=635&tbm=isch&tbnid=7oCqne0BXzT3KM:&imgrefurl=http://labs.medicine.ucsf.edu/chrislau/GFP.html&docid=_uorF4Iy082FAM&imgurl=http://labs.medicine.ucsf.edu/chrislau/images/pup2.jpg&w=1350&h=765&ei=zcmEULXWEaz4igL65IDABw&zoom=1&iact=hc&dur=918&sig=117709133158191246935&page=1&tbnh=132&tbnw=239&start=0&ndsp=23&ved=1t:429,r:19,s:0,i:191&tx=157&ty=83&vpx=636&vpy=186&hovh=169&hovw=298 http://www.google.com/imgres?hl=en&biw=1525&bih=635&tbm=isch&tbnid=xi1aYXGHoppOUM:&imgrefurl=http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html&docid=6idiQbUsIkoeAM&imgurl=http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/P/plasmids.png&w=290&h=235&ei=CsqEUM2oNsPoigKxhoCQDA&zoom=1&iact=rc&dur=424&sig=117709133158191246935&page=1&tbnh=139&tbnw=184&start=0&ndsp=23&ved=1t:429,r:1,s:20,i:200&tx=114&ty=44 http://www.google.com/imgres?hl=en&biw=1525&bih=635&tbm=isch&tbnid=gep5tCBgRwueNM:&imgrefurl=http://www.neb.com/nebecomm/tech_reference/modifying_enzymes/feature_article_sample_preparation.asp&docid=diGZ5KpzzXKJxM&imgurl=http://www.neb.com/nebecomm/tech_reference/modifying_enzymes/images/LibConstWorkflow_509.jpg&w=588&h=381&ei=R8qEUNuCKOvliwLmyIHYDg&zoom=1&iact=rc&dur=471&sig=117709133158191246935&page=1&tbnh=139&tbnw=214&start=0&ndsp=24&ved=1t:429,r:16,s:0,i:143&tx=130&ty=72 http://www.google.com/imgres?start=0&num=10&hl=en&biw=1525&bih=635&tbm=isch&tbnid=rD3g5A_cRqEEpM:&imgrefurl=http://www.nij.gov/training/courses/analyst-str-data.htm&docid=1j3QH7DOLxSY-M&imgurl=http://www.dna.gov/rawmedia_repository/3850cb6b_a2b2_4f5b_a879_423801297534&w=450&h=360&ei=ncqEUPHqKsPUiwKzzYCgAQ&zoom=1&iact=rc&dur=600&sig=117709133158191246935&page=1&tbnh=148&tbnw=212&ndsp=22&ved=1t:429,r:1,s:20,i:7&tx=124&ty=56 http://www.google.com/imgres?num=10&hl=en&biw=1525&bih=635&tbm=isch&tbnid=T27WDJqy5RDnwM:&imgrefurl=http://plantpathology.ba.ars.usda.gov/virtualgel.html&docid=mrrTMjLU2SijdM&imgurl=http://plantpathology.ba.ars.usda.gov/graphics/pclass/virtualgelfrontpage.jpg&w=440&h=422&ei=_MqEULiPEo6vigL8u4GYCg&zoom=1&iact=hc&vpx=1253&vpy=300&dur=1287&hovh=220&hovw=229&tx=148&ty=147&sig=117709133158191246935&sqi=2&page=1&tbnh=143&tbnw=149&start=0&ndsp=21&ved=1t:429,r:13,s:0,i:134 http://www.google.com/imgres?start=0&num=10&hl=en&biw=1525&bih=635&tbm=isch&tbnid=2mWyJD2uO_VvGM:&imgrefurl=http://nursinglink.monster.com/training/articles/530-genetic-mapping&docid=StYWkS_UbKseOM&imgurl=http://nursinglink.monster.com/nfs/nursinglink/attachment_images/0000/0782/723_crop380w.jpg%253F1212559625&w=380&h=250&ei=UsuEUIWrNsa_igLZ-ICgAg&zoom=1&iact=rc&dur=345&sig=117709133158191246935&page=1&tbnh=141&tbnw=221&ndsp=23&ved=1t:429,r:0,s:20,i:4&tx=99&ty=66 http://www.google.com/imgres?hl=en&biw=1525&bih=635&tbm=isch&tbnid=7bSmvRChBr9cFM:&imgrefurl=http://www.sciencephoto.com/media/211335/enlarge&docid=4pLAXFNpowfToM&imgurl=http://www.sciencephoto.com/image/211335/large/G2100843-Molecular_beacon_genetic_probe-SPL.jpg&w=530&h=342&ei=28uEUIevMqWUiQLa8oDwDg&zoom=1&iact=rc&dur=443&sig=117709133158191246935&page=1&tbnh=132&tbnw=228&start=0&ndsp=23&ved=1t:429,r:13,s:0,i:107&tx=110&ty=8
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