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CLP400 - Sample Collection and Smear Preparation

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Kirsti Clarida

on 24 January 2013

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Transcript of CLP400 - Sample Collection and Smear Preparation

Otitis Externa Sample Collection
Smear Preparation IMPRINTS To blot the fresh, dry edge of tissue sample along a microscope slide. PROS
- living or excised tissue
- easy to collect
- minimal restraint during collection of living samples CONS
-collect fewer cells
- more contaimnated
- shows secondary infection (impression smear) SCRAPINGS To pass a scalpel blade over tissue or lesion to "scrape" cytologic sample for evaluation PROS
- collects many cells
- useful on firm lesions
- living or excised tissues CONS
- more difficult to collect
- superficial samples
- secondary infection displayed SWABS To collect a sample with the use of a swab Pros
- best when other collection methods won't work
- excellent for fistulas, nose, ears, vaginal cytology CONS
- technique can result in cellular damage or destruction FINE NEEDLE BIOPSY To use a needle inserted within the mass for collection TWO METHODS: aspirate The application of negative pressure, using needle and syringe, while inside mass to draw sample. non-aspirate To use only the passage of the needle into the mass as a means of collection-no negative pressure is used. PROS
- avoids contamination
- may see fewer cells PROS
-avoids contamination CONS
- may see fewer cells
-often only enough for one sample, must repeat collection Blood Smear aka: Wedge or Line Smear Compression Smear aka: Sandwich or Squash smear - best when sample isn't concentrated (i.e. low cellularity)
- requires practice to perfect
- USES: FNB (aspirate and non), centesis, T/B wash - great cytologic smear
- improper technique can crush or tear cells apart
- USES: FNB (aspirate and non), centesis, T/B wash and scrapings Starfish Smear - does not damage fragile cells

- good for viscous samples

-thick fluid around cells can hinder spreading and evaluation

- USES: FNB (aspirate and non), centesis, T/B wash Combination Smear Squash & Wedge smear - works with variability in cells

- WEDGE is best for fragile cells

- SQUASH is best for clumps of cells

- MIDDLE is best for low cellularity CYTOLOGIC
SAMPLE Lesions
Discharge Fluid Organ Tissue Now What? prepare samples immediately following collection

knowing where they are going can help you decide how to prepare them

when in doubt - call the lab! Rules of Thumb
Stain can be a pain! - many labs use preferred stains and methods when assessing samples. Check with your lab.

A picture is worth a thousand words - be sure to stain at least one sample for in-house assessment to be sure you have quality slides

The more the merrier - well a few more anyway. Send multiple slides. Let's take a quick peek at selecting your stain STAINS New Methylene Blue Nuclear detail amazing
Cytoplasm staining is weak
RBCs faintly stain (almost undistinguishable)
Therefore.... best used on samples with dense cell clumps and/or samples with greater RBC contamination Rowmanowsky Stains (Diff-Quick, Wright's) very common, easy to store, and use
cytoplasm stains really well
organisms also stain really well
nuclear detail is only sufficient
mast cell granules may not stain well at all and lead to misinterpretation as macrophages

Therefore... best used as an overall stain, suited for most practices
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