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kelsey pocsik

on 21 January 2013

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Transcript of Microbiology

Disease 0157;H7 Infectious Microorganism E.coli has an outer layer, Glycocalyx
This bacteria uses peritrichous flagella, fimbriae to move and adhere to other substances
E.coli's cell wall is gram-negative
E.coli does not activate all of its genes at the same time, for example, it only activates genes for lactose catabolism when lactose is detected in the environment. E.coli is a Gram-negative, rod-shaped bacterium (bacillus) that is commonly found in the lower intestine of warm-blooded organisms. (www.wikipedia.com)
German pediatrician and bacteriologist, Theodor Escherich discovered the bacterium in 1885. (Nordqvist, 2007) Generally the disease has to run its course.
Drinking plenty of liquids to replace the body fluids lost through vomiting and diarrhea.
Loperamide (OTC) may also be taken to reduce motility of the large bowel to allow more water absorption.
Because E.coli is gram-negative it is naturally resistant to many antibiotics used to treat gram-positive infections.
(Gould, 2010) On average about 440 cases of E.coli are reported annually in Canada.
E.coli O157; H7 causes 70,000 people with symptoms of diarrhea each year.
From 2006 to 2011 the total cases of E.coli dropped from 978 to 482, showing an increase of awareness in society.
About 5 to 10% of those who get sick from E.coli O157; H7 develop hemolytic uremic syndrome which can be fatal.
XL Foods contamination.
Several people became sick after eating the contaminated beef.
Some problems were identified during this occurrence:
- Incorrectly sanitized equipment
- Cows and bulls dragging along the floor
- Condensation dripping onto carcasses, for which CFIA held 315 carcasses back from the production line Sam Hofman and Kelsey Pocsik E.Coli Disease Diagnosis Positive Side Disease Treatment Statistics Cool Facts Quiz Time! References Q: What type of cell wall does
E.coli have?? A: Gram-negative cell wall Q: Which is not a symptom of E.coli O157;H7??

A) Fever
B) Diarrhea
C) Vomiting
D) Loss of Hearing Q: Which of these methods is can cause
E.coli O157; H7??

A) Raw Meat
B) Pasteurized Milk
C) Clean Water
D) Maintaining Proper Hygiene A: A) Raw Meat contains pathogenic bacteria unless destroyed by cooking it. Which type of E.coli is considered
pathogenic?? E.coli O157; H7, if both O and H antigens are present the E.coli becomes pathogenic. Q: T/F Does the antibiotic penicillin help with
destroying E.coli O157;H7?? A: False!! This bacteria outer membrane prevents the movement of penicillin to the underlying layer. (www.youtube.com) E.coli infections are usually caused by eating contaminated food and water or coming in direct contact with someone who is carrying it. (Public Health Agency of Canada, 2013)
This bacteria is categorized under zoonosis. (Symbiosis, 2011)
Some symptoms include: dehydration, diarrhea, nausea and other life threatening complications.
There are three categories of E.coli that cause food borne illness:
1. Enterotoxigenic
2. Enteropathogenic
3. Enterohaemorrhagic
(Gould, 2010) Diarrheal stool samples will be analyzed microbiologically.
There are other expensive methods to diagnose an infection with E. coli, but stool stamples are the main use of diagnosis.
After diagnosis, some patients will be monitored more closely. Monitoring involves periodic collection of urine samples or blood samples. Some strains of E.coli exist as part of normal flora in the human gut and have many beneficial functions.
E.coli colonizes the human bowel within a few weeks of birth.
Many scientists today use E.coli as a platform to produce human therapeutic glycoproteins which are commonly used for medications. A: D) Loss of Hearing Gould, D. (2010). Causes, prevention and treatment of Escherichia coli infections. Nursing Standard, 24(31), p50. Academic OneFile. Web. 15 Jan. 2013

Lerner, K. L., & Lerner, B. W. (2003). World of Microbiology and Immunology (vol 1.). Farmington Hills, MI: The Gale Group Inc.

Nordqvist, C. (2007). What is E.coli?. Medical News Today. Retrieved January 16, 2012, from http://www.medicalnewstoday.com/articles/68511.php

Pons, Luis. (2004). Targeting E.coli infections at their source. Agricultural Research 52(8), 14. Academic OneFile. Web. 9 Jan. 2013.

Public Health Agency of Canada (2013). E. Coli. Retrieved January 16, 2013, from http://www.phac-aspc.gc.ca/fs-sa/fs-fi/ecoli-eng.php

Riciuon, A. (2011, July). Escherichia Coli (E. Coli) Infection – Transmission, Symptoms And Diagnosis. Retrieved January 16, 2013, from http://www.huffingtonpost.ca/news/xl-foods-recall

Symbiosis: The Pearson Custom Library for the biological science. (2011). Boston, MA: Pearson Learning Solutions.

XL foods to destroy all meat from E.coli recall, (2012, October). Retrieved on January 16, 2013 from http://www.cbc.ca/news/canada/story
/2012/10/20/xl-foods-destroy.html (Microbiology PowerPoint, 2013) www.google.ca www. google.ca www.google.ca www. google.ca www.google.ca www.youtube.com) (Symbiosis, 2011) (Riciuon, 2011) (Nordqvist, 2007) (Public Health Agency of Canada , 2013) (The Canadian Press, 2012)
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