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Biology Project

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Laylo Abdurahmonova

on 25 April 2014

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Transcript of Biology Project

1. Rinse all of the 5 aquariums to make sure it's clean.
2. place gravel into the aquarium until it fills 1/8 of the tank.
3. Repeat step 2 for all of the 5 aquariums.
4. Pour 2 gallons of clean water to all of the 5 tanks.
5. Check the temperature with thermostat to make sure the water temperature the fish is temporarily held in matches the temperature in the tank so that the fish will not be in stress.
(Install the heater to use when needed)
If Marimo algae balls are placed into male Betta fish's aquarium life, then the optimal amount of algae that would effect the weight of male Betta Splendens is the Marimo with 10.16 centimeters diameter because the marimo ball is capable of improving the water quality (without getting in the fish's way) which the fish requires to live healthy and being healthy will increase the size meaning the weight increases.
Procedures continued...
6. Keep the temperature constant between 75-86 degrees
7.Carefully place the fishes in their aquariums.
8. Place one marimo ball to each of the 4 aquariums and record which tank contains which size of the marimo.
9. Do not place a marimo ball to one of the tanks as to that tank will be the controlled to compare back.
10. Feed the fishes twice a day,(every morning and evening) (6 days a week)2 to 3 pellets and let them fast once a week so they aren't stuffed.

Procedures continued...
11. Check the temperature twice a day (every morning and evening) to make sure it's at constant for every fish.
12. Repeat the steps 7 and 11 for 3 weeks.
13.This experiment should last 3 weeks.
14. To measure use the big plastic cup to grab 1 cup of water and measure the weight using the gram weight scale.
15. empty the cup and measure the cup itself. Record the weight.
16. Grab one cup of water and use a net to grab the fish from the tank and quickly place it into the plastic cup where there is the one cup of water.
Biology Project
By: Laylo Abdurahmonova

Using a Marimo (Diameter in cm), What is the amount of Algae that will effect the weight of male Betta Splendens (In grams)?

-Independent Variables: Marimo algae moss ball
-Dependent Variables: The weight of Male Domestic Veiltail Betta Splendens (in grams)
-Controlled Variables: Age of the fish, the beginning weight of the fish, species and gender of the fish, species of the Marimo balls, beginning quality of the water, size of the tank, temperature of the water, food type for the fish, the amount of food fed during the experiment, amount of light received to both the fish and marimo and top lid has holes on all the aquariums .
- 5 Male Domestic veiltail Betta Splendens all same weight(1.6 grams)
- 4 marimo balls all different sizes. 1st algae is 6.35 cm in diameters, 2nd is 20.32 cm, 3rd is 10.16 cm and 4th is 4 cm.
-5 same tanks that hold 2 gallons of water(16inLx12inWx19inH)
-2 Gallons of clean water for each of the tanks.
-Betta fish food (Aqueon Betta pellets Betta Food)
-Gravel (same color) for all 5 tanks filling 1/8th of the tank.
-a plastic big cup.
-Gram weight scale
-A container
-a plastic fish net( to grab the fish)
Procedures continued...
17. Measure weight of the cup and record it.
18. Subtract the weight of 1 cup water and
the weight of the cup from the weight of the
cup that contained one cup water and the fish.
This will give you the measure of the fish.
19. Repeat the steps 15 to 18 for each of the fishes
in the tanks.
20.Repeat the steps from 13 to 19 three times
for 3 weeks. Each time at the end of a week to
record the fish's weight's changes.
21. Record fish's weights' data on a table chart.

Table Chart
Data Analysis
Extra information
If this experiment was ever done, the first tank should have a fish with
marimo ball that is 6.35 cm long.
The second tank should have a fish with marimo 20.32 cm long.
The third tank should have a fish with marimo ball that is 10.16 cm long.
The forth tank should have a fish with a marimo that is 4cm long.
The fifth tank should have a fish without a marimo ball.

Data Analysis

1. I might have expected the first fish to rarely to no change in terms of size and weight because the water quality is not that well. The fish uses its labyrinth to breathe at the surface sometimes. Other than that the activity is normal. The marimo ball cleans the water quality but not perfectly because it is too small and it's the only plant responsible to clean the nitrates itself. As a plant, it goes through cellular respiration to oxidize the water but it's not enough.
2. I would expect the second fish to decrease in weight because the marimo ball takes up more than half of the tank space limiting the space the fish requires. Also since the Marimo ball is big, it suchs up more nitrates, requires more food( survives with the waste the fish creates and it is tolerant to salt, and pH adaptable. The fish will have less space and struggle keeping up with things. The marimo also clears fish food fast so the fish might become hungry.
Data Analysis (continued)
3. I would expect the third fish to be the healthiest
fish of all because the marimo it lives with
takes up 1/4 of the tank which is perfect because
the fish still has space to swim and the plant can still
move around and collect waste and it is big enough to
filter the tank water. The plant also uses cellular respiration to oxidize the water so the fish doesn't swim in the surface to breathe. Also, marimo cleans out the extra food the fish leaves behind and prevents bad algae from growing while still not harming the fish. Healthy fish then starts weighing more.
Data analysis (Continued)
4. I would expect the fourth fish to be less active than
the third fish because the marimo is too small to
clean the whole tank, there are still some waste left
over and the oxygen produced is little for the fish so
the fish tends to be breathing at the surface. The
water quality is not very well. The weight of the fish
doesn't change because it is still receiving food but
the water quality is not exactly what the fish wants.
Data analysis( Continued)
5. The fifth fish would probably have some sort of disease or close to having malfunctioning organ because the water quality is really bad without anything to clean it. The left over food starts to ruin the water quality. The fish's health is not in good condition. The fish could be in the state of dying because Betta fishes require a water with good quality.
Conclusion: Accepted
My hypothesis was proven right after
conducting this experiment. Betta fishes
do live better in the aquarium with marimo
balls that have 10.16 cm length because it
is actually perfect amount to clean the water
and still leave some space for the fish to swim.
With marimo in 2 gallon water, it was proven
that it improves the water quality without requiring
a filter. Also it was safe for the fish but filters can be dangerous. I conclude that it has little impact on the field of science because there are other technologies
being created specifically for Betta fish so that
they improve the water quality while not harming them.
Also there can be other plants that take up less space
but still manage to clean the water.
Conclusion: Incorrect
My hypothesis was incorrect. Actually
the size I predicted didn't effect the fish's
weight. This would not have great impact
on scientists because I have been only focusing
with the
on the weight of the fish but not
specifically whether it would increase the weight
or decrease it. If scientists choose to do it on whether
it would increase the weight, they would know that the
diameter size I chose might still not be enough to
support the whole tank. If scientists choose to
do it on whether it would decrease, they would know
that 10.16 cm Diameter of marimo is too much of
good to decrease the weight of the fish. They would know that 10.16 is not a bad amount that can decrease
the fish's weight. They could narrow down the numbers now that they know exactly where the numbers could stand. If I wanted to further conduct a research on this, I would alter the question to narrow it down more. I would choose to do it on the effect of increasing of the weight because that way I can focus more on what to expect.
Research source #1: Marimo Balls
- Marimo balls appear at the bottom of the lakes and are not adapted to direct sunlight.
-The water needs to be completely changed every once a week to keep the marimo healthy.
- Marimo eats wastes like ammonia and nitrates.
- Marimo prefers to be in cooler temperature.
Research source #2: Marimo Balls
- Marimo Ball is a plant and uses cellular respiration.
- It also sucks nitrates up including ammonia.
- They terminate the growth of bad algae.
-They help the fish with nitrogen cycle.
-They are adapted to different pH values.
-They also tolerate salt.
-They grow slow.
Research source #3: Betta Splendens
- Bettas need to be kept in the temperatures between 78 and 86 degrees.
-Bettas are carnivores.
-The perferable condition of the aquarium for bettas are to be well-filtered.
-They need to have an open surface to breathe from their labyrinths.
-They like to eat food while it is still at the top.
- In water with bad condition, the fish swims to the surface frequently to breathe some air.

Research source #4: Bettas
- Male bettas should be kept seperately because they can
be very aggressive and fight resulting in death.
- Stable water temperature, pH value and quality is very
-Signs that show a fish is healthy is their colors are vibrant, they eagerly eat their food, they tend to be active and are aggressive towards outside events.
- Signs that show the fish is sick are their loss of appetite, difficult breathing and they hang out at the bottom of the tank.
- Crowded space causes stress and disease to them.
Research source #5: Cleaning the tank
-have a lid with net or top cover that has wholes because Bettas jump.
- They shouldn't be left alone for more than 3 days.
- They shouldn't be put in contact with the sun.
-Their fins are so delicate.

Procedure (continued)
20. To clean the tank use the plastic cup to carefully grab water
from the tank and pour it out. Keep doing this
until there is about 25% water left with the fish.
Be careful not to hurt the fish.
21. Carefully use that plastic cup to grab the fish.
Grab the marimo ball with clean hands and set it
in a container and
pour the rest of the water out.

Procedure (continued)
24. Put the gravel between your hands and rub them against each other. Do not use soup.
25. Rinse the tank out and refill it with 2 gallons of clean water. make sure the temperature of the water matches the temperature the fish is in with the plastic cup. Place the gravels back in.
26. Add some water to the container. Squeeze the marimo gently to clean it.
27. Carefully place both marimo and the fish to the tank.
28. Repeat the steps from 22 to 27 for all of the tanks.
Procedure (continued)
29. Repeat the steps 22 to 28 once a week for 3 weeks.
Research source #5:

Research source #6:
Difference between male and female fish is that male betta fish tend to have longer and pretty fins
which takes up more weight than female's.
-Betta fishes have a hormone that can stunt their growth and after a while result in death. This stunt can be caused by less water change in their tanks.
-Betta fish are capable of jumping off their tanks so top lid should be on but have holes so they can breathe.

1."Tropical Fish for Freshwater Aquariums: Betta - Male." Tropical Fish for Freshwater Aquariums: Betta - Male. Foster and Smith INC, 1997. Web. 19 Apr. 2014. <http://www.liveaquaria.com/product/prod_display.cfm?c=830+832+990&pcatid=990>.
2. "Betta Anatomy." Betta Anatomy. N.p., 2004. Web. 19 Apr. 2014. <http://www.aquaticcommunity.com/bettafish/anatomy.php>.
3. "Marimo Balls: Care & Info." RSS. N.p., 2 Apr. 2014. Web. 19 Apr. 2014. <http://aquariadise.com/marimo-balls-care-info/>.
4. "Betta Splendens Care Sheet." Petco. Petco Animal Supplies Inc, 2005. Web. 19 Apr. 2014. <http://www.petco.com/caresheets/fish/Betta_Male.pdf>.
5. Flash123. "How to Clean a Betta Fish Tank." WikiHow. Ed. Jordan and Spirios. Creative Commons License, n.d. Web. 19 Apr. 2014. http://www.wikihow.com/Clean-a-Betta-Fish-Tank
6. Zidanmahardika. "How to Make Betta Fry Grow Faster." Betta Fish Care. N.p., 2012. Web. 21 Apr. 2014. <http://bettafishzidan.wordpress.com/2012/11/16/how-to-make-betta-fry-grow-faster/>.
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