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HISTOLOGICAL STUDY OF THE EFFECT OF GOLD NANOPARTICLES ON LIVER CELLS OF ADULT ALBINO RATS

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dina rostom

on 9 August 2014

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Transcript of HISTOLOGICAL STUDY OF THE EFFECT OF GOLD NANOPARTICLES ON LIVER CELLS OF ADULT ALBINO RATS

HISTOLOGICAL STUDY OF THE EFFECT OF GOLD NANOPARTICLES
ON LIVER CELLS OF ADULT ALBINO RATS
Introduction
Applications of AuNPs
In the current study
why Liver?
what is "NANO"?
Gold
Types of nanoparticles
A human hair is 80,000 nanometers wide
A sheet of paper is about 100,000 nanometers thick
Introduction
Introduction
Thesis submitted to the Faculty of Medicine
Alexandria University
In partial fulfillment of the requirements for the degree of

Master of Basic Medical Sciences
In
Histology and Cell Biology


Assistant Prof. Dr. Fardous Serour Kotb
Assistant Professor of Histology and Cell Biology
Faculty of Medicine
University of Alexandria
It is defined as a particle with at least one dimension <100 nm in diameter.
A Greek word, meaning "dwarf"
Aim Of The Work
The present work aimed to study the possible histological alterations in hepatic cells of male albino rats after the administration of different sizes of AuNPs.
Material and Methods
Material and Methods
50 adult male albino rats
Group I
(control)
20
Group II
(experimental)
30
Subgroup
(Ia)
Subgroup
(Ib)
Subgroup
(IIa)
Subgroup
(IIb)
Subgroup
(IIc)
Preparation of gold nanoparticles
Results
1- Characterization of the prepared gold nanoparticles
1. TEM
2. Ultraviolet-visible absorption spectroscopy(UV-Vis)
3. Nano Zeta sizer particle analyzer
10nm AuNPs
20nm AuNPs
50nm AuNPs
4. Fourier transform infrared spectroscopic analysis (FTIR)
5. X-Ray diffractometer (XRD)
2-Biochemical study of Liver Functions Tests
Material and Methods
150-200 g
6-8 weeks
Supervisors
Prof. Dr. Safinaz Hussein Safwat
Professor of Histology and Cell Biology
Faculty of Medicine
University of Alexandria
Prof. Dr. Moushira Aly Reda El Heneidy
Professor of Histology and Cell Biology
Faculty of Medicine
University of Alexandria
Prof. Dr. Thanaa Ibrahim Ahmed Shalaby
Medical Research Institute
Faculty of Medicine
University of Alexandria
Toxicity of gold nanoparticles
shape
size
Spherical
10, 20, 50nm
Negative surface charge
surface coating
3-Histological results
I. Light microscopic results: H and E
H and E
Immunohistochemistry
using caspase-3


•The present study constitutes an experimental model for the
synthesis
of different sizes of gold nanoparticles (10, 20 and 50nm).

RECOMMENDATIONS
1.Experimental work in the field of nanomedicine should include improved and comprehensive overview of
adsorption, distribution, metabolism and elimination
of AuNPs.

CONCLUSION
Subgroup Ia
Subgroup Ib
Subgroup IIa (10 nm AuNPs)
Subgroup IIb (20 nm AuNPs)
Subgroup IIc (50 nm AuNPs)
Control
Subgroup IIa
Subgroup IIb
Subgroup IIc
Subgroup Ia
Subgroup Ib
Control
Subgroup IIa
Subgroup IIb
Subgroup IIc
Subgroup Ia
Subgroup Ib
Subgroup IIa (10 nm AuNPs)
Subgroup IIa (10 nm AuNPs)
Subgroup IIb (20 nm AuNPs)
Subgroup IIb (20 nm AuNPs)
Subgroup IIc (50 nm AuNPs)
Subgroup IIc (50 nm AuNPs)
Group I (control group)
Immunohistochemistry
using caspase-3
II. Electron microscopic results
The prepared AuNPs were characterized using Ultra violet-visible spectroscopy (UV-Vis spectroscopy), Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Zetasizer Particle Size and Stability Analyzer
every material can give many materials as the size decreases
with criteria differing from their bulk
Surface plasmon resonance (SPR)
Why Gold nanoparticles?
stability
ease of preparation and detection
by electron microscopy
unique optical properties
good biocompatibility
After the sacrifice of the rats
Morphometric study
2.
Longer term
studies are needed to verify the potential cytotoxic effects of AuNPs on different cell types.

3.Further studies are required to examine the effects of a broader size range of gold nanoparticles on
different organs
of rats.

4.Studies should extend to investigate
oxidative stress markers
in different organs exposed to AuNPs.

5.More studies should be conducted to examine the effect of
coating materials
other than trisodium citrate dihydrate on the liver and other body organs.

•Morphological
characterization
of the prepared gold nanoparticles proved the success of the work protocol applied in the study.

•Small sized AuNPs provoked
drastic
histological and biochemical changes in the rat hepatocytes in comparison to the larger ones.

•AuNPs of size 50 nm provide
hope
for therapy and diagnosis of a variety of diseases.

•Examination by the transmission electron microscope provided strong
ultrastructural evidence
for the changes observed by the light microscope.

•The
immunohistochemical analysis
conducted in this study proved the role of AuNPs in inducing apoptosis in the liver cells.

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