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Enhanced Methods for Doubled Haploid Production in Triticum
Transcript of Enhanced Methods for Doubled Haploid Production in Triticum
Heartland Plant Innovations and the Kansas Wheat Innovation Center
Creating DH lines is a multi-stage procedure that takes an average of 52 weeks to compete
Process begins with growing clients' seeds to flowering stage
The Importance of Wheat Haploids
Image credit: E. Holbrook, RM Magazine
for Doubled Haploid Production in
T. SUELTER, F. Chumley, R. Berard, and H.N. Trick
Current growth media is not optimized for embryo growth and development.
Chromosome doubling with colchicine treatments are a major stress to plant that can be difficult to recover from.
A Closer Look at Rescue
Normal seeds compared to haploid seeds 2 weeks into development
Glumes and awns are clipped from the spike and the anthers are stripped off each flower
Maize pollen is dusted onto the wheat stigma, inducing haploid embryogenesis
After two weeks of development, haploid seeds are threshed from the flower spike and sterilized
Embryos are excised from the haploid seeds and cultured on MS medium
Haploid plantlets are allowed to grow to the three leaf stage, and vernalized for eight weeks
Haploid plantlets are transplanted to the greenhouse to grow until they begin tillering
Plants are removed from potting medium, and soaked in a solution of colchicine and repotted
Plants recover in growth chambers before returning to the greenhouse to grow to maturity
After reaching maturity, DH lines are threshed and shipped to clients for field testing.
Image credit: J. McGowan, HPI
Seed coat opened on normal and halpoid seeds.
Excised normal embryo compared to haploid embryo on scalpel tip
Wheat accounts for 20% of all the calories consumed by humans worldwide
World trade of wheat is larger than all other crops combined
Food production must to increase by over 70% by 2050 to feed 9 billion
Haploids reduce number of selfing generations before selection
Improve chances of selecting desired recombinant
Select and confirm in the same season
Combined time savings can dramatically cut time for releasing new wheat varieties
Accelerated gene discovery
Doubled haploid production in wheat and other food crops (triticale, sorghum, millet, etc.) must grow to meet the demand of breeding programs.
Overcoming the biological constraints that reduce efficiency of embryo culture and chromosome doubling is integral to achieving that growth in production.
Image credit: Kansas Wheat
Image credit: Kansas Wheat
Repeated Self Crossing
Conversion Rates at HPI, a Three Year Average:
For every head emasculated we return, on average, 1.9 lines.
For every embryo rescued about 37% will return a developed line.
Turn around times for our orders has gone from 15.2 to 13.7 to 13.0 months