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Orchid Hybridization

SBSMP biology
by

ruth keh

on 9 November 2010

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Transcript of Orchid Hybridization

Orchid Hybridization Artificial pollination of orchid AIM: To develop a bigeneric species of orchid by
hybridization THAT signifies and represents
the Victorian Culture of VJC. the orchid hybrid would ‘shine’ and stand out from the rest of the plants in terms of its presentation and colours. It would look vibrant and very appealing. the orchid hybrid would stand tall (strong stems) to symbolize a Victorian attempting to be the best he/she can be. As such, we portray a resilient plant that is able to adapt to the environment well. the plant should look vibrant, friendly and blooming to symbolize the atmosphere in VJC. VICTORIAN COLOURS The Victorian colours (yellow, red) will be used as a desired outlook for the orchid after hybridization. By crossing two plants with the desired characteristics in an ideal environment, the hybridized plant would have somewhat the desired characteristics.
The process would be repeated a few times to increase our chances of getting the plant we want.

Our desired characteristics are yellow petals with maroon coloured dots and resilient to the tropical environment all of which display the Victorian spirit. HYPOTHESIS ARTIFICIAL POLLINATION Parent plants of hybrid will be selected.
Collecting pollen from one parent plant’s flower on a needle, and inserting it into the stigmatic surface of a flower from the other parent plant.
If fertilization is successful, the ovary of the flower will develop into a seed pod (fruit). Experiment involves artificial pollination of a orchid plants and growth of plant hybrids by the method of plant tissue culture.
Propagation of plants under sterile conditions.
BUT WHY PLANT TISSUE CULTURE? production of exact copies of plants that produce particularly good flowers, fruits, or have other desirable traits. production of plants without seeds or necessary pollinators to produce seeds. production of plants in sterile containers that allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens. production of plants from seeds that naturally have very low chances of germinating and growing, i.e.: orchids Plant tissue culture relies on the fact that many plant cells have the ability to grow into a whole plant.
Used to generate a new plant on culture media given the required nutrients and plant hormones. Plant cells can be grown in tissue culture systems if there is/are:

•a growth medium containing organic and inorganic compounds to sustain the cells
•aseptic conditions during culture to exclude competition from microorganisms the grower can choose the parent plants and decide whether to pollinate the plant : own pollen (selfing) pollen of a different plant of the same species (cross pollination) another species (hybridizing). MATERIALS MATERIALS 2.Plant tissue culture media (agar with necessary nutrients for optimum growth of orchid plants) MATERIALS 3.Detergent
4.Ethanol
5.Clorox solution For sterilisation Apparatus 1.Parafilm
2.Sterile Petri-dishes
3.Lamina flow cabinet
4.Autoclave mature seed pods collected and its seeds sterilized to kill micro-organisms on surface of plant tissue before culturing. METHOD/PROCEDURE washing the seed pod in dilute detergent, soaking it in ethanol to kill bacteria, soaking the seed pod in Clorox solution to kill fungi rinsing in distilled water to remove chemicals present on the surface of the seed pod. seed pod is then grown in agar media containing necessary nutrients for optimum growth. Once the seed pod has been sterilized, it will be
cut longitudinally into two halves dust-like orchid seeds will be placed onto the agar surface of each Petri-dish, sealed
with parafilm. there will be many set-ups of seed cultures (at least 10) made to test out the different kinds of hybrids possible. hybrid develops shoots, old leaves are trimmed off. and bud is removed inoculated on agar slants at 12 hours per day photoperiod under low-light intensity. These meristems grow into protocorms
(easily sub-cultured and multiplied on fresh agar mediums.) Protocorms will be grown in Petri-dishes.
Separating large clumps of protocorms - cutting them into smaller pieces in the lamina flow cabinet, so that the shoots will remain sterile. Cultures will be incubated at 20 +/- 2 oC,
under 12 hours per day photoperiod
and observed at weekly intervals.

Orchid plants can be sub-cultured onto fresh medium
(Phytamax Orchid maintenance medium P6668) to
encourage further growth. THANK YOU!!!! ORCHIDS http://en.wikipedia.org/wiki/Plant_tissue_culture
http://www.liv.ac.uk/~sd21/tisscult/what.htm
notes on orchid hybridization BIBLIOGRAPHY
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