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Fundamentos de Biologia Molecular e Genética

Aula na disciplina MO640 - Biologia Computacional, Campinas, 10-12 de agosto de 2015.
by

Felipe Rodrigues da Silva

on 14 August 2015

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Transcript of Fundamentos de Biologia Molecular e Genética

1859
Charles Darwin
On the Origin of Species
1950
Erwin Chargaff
%A = %T e
%G = %C
1952
Rosalind Franklin e Maurice Wilkins
X-ray diffraction
1957
Francis Crick
1928
Frederick Griffith
Transforming Principle
Sanger
enzymatic procedure
.
A ordem das bases é determinada pelo tamanho dos fragmentos obtidos através de reações de
polimerização
na presença de
dideoxinucleotídeos
Maxam & Gilbert
procedimento químico
G
A
T
C
Mestrado, 1996:
Doutorado 2001:
1975
Sir Edwin M. Southern
Southern Blot
PCR
http://content.dnalc.org/content/c15/15475/19_polymerase_chain_reaction.mp4
Animação (em 3D):
2 general principles:
Sequence Hypothesis
Central Dogma
1953
Rosalind Franklin e Maurice Wilkins
difração de raio-x
Ciclo
Cópia
Delimitada
PCR
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1.048.555
1.073.741.793
34.359.738.332
1.099.511.627.735
1.125.899.906.842.570
Felipe Rodrigues da Silva
Lab. de Bioinformática Aplicada - LBA
Lab. Multiusuário de Bioinformática - LMB
Embrapa Informática Agropecuária
felipe.silva@embrapa.br
felipe.silva@embrapa.br
Fundamentos de Biologia Molecular e Genética
EST – Expressed Sequence Tag
DNA
RNA
protein
Central Dogma of Biology
cloning
sequencing
Genomics
http://www.lmb.cnptia.embrapa.br/share/public/felipe/NGS2015
NGS
6 x 100pb / 8 dias
96 x 800pb / 12 horas
96 x 800pb / 12 horas
x número de máquinas...
6.000.000.000 x 100pb / 10 dias
A little bit of history
http://en.wikipedia.org/wiki/Sequence_hypothesis
http://en.wikipedia.org/wiki/Central_dogma
Gene, Double Helix 40th anniversary
Watson: Looking forward
http://www.cnptia.embrapa.br/~felipes/PROCISUR2013/LF.pdf
Crick: Looking backwards
http://www.cnptia.embrapa.br/~felipes/PROCISUR2013/LB.pdf
1993
135 (1993)
309-315
15-18
Genome Shotgun
Dogma Central da Biologia
Illumina
(2006)
CGTAC…
Phasing
Polimerização de DNA
Sanger (clássico?)
NGS (
Next Generation Sequencing
)
Sequenciamento de DNA)
Genômica
PCR
Full transcript