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Experimental Investigation of Bioethanol Production from Durian Seed by Fermentation

Final Year Project 2 Presentation

Aditiya Harjon

on 9 February 2014

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Transcript of Experimental Investigation of Bioethanol Production from Durian Seed by Fermentation

Stock Composition:
10 ml hydrolysis stock
90 ml RO water
Nutrient composition:
Experimental Investigation of Bioethanol Production from Durian (Durio zibethinus) Seeds by Fermentation as the New Potential Feedstock of Second Generation Production
Aditiya Harjon Bahar (ME 086549)
The Idea of Bioethanol (Biofuel)
Instead of using feedstock to be converted into bioethanol,
feedstock waste is used.
This is possible since bioethanol production uses principle of carbohydrate bond breaking and fermentation.
Hydrolysis (enzymatic)
Hydrolysis (enzymatic)
Thank you!
Q&A session is now opened
"The fuel of the future is going to come from fruit like that sumac out by the road, or from apples, weeds, sawdust - almost anything."
- Henry Ford, 1925
More crops converted into bioethanol, less feedstock, higher food price.
source: International Energy Agency
Supv.:T. M. Indra Mahlia, DR. Prof.
Final Year Project 2
Universiti Tenaga Nasional

Petroleum Refinery
1st Generation Fuel
2nd Generation Fuel
Crude petroleum
Corn, sugar cane, vegetable oils, etc.
Bioethanol, biodiesel, sugar alcohol
Environmentally friendly, economic, social security
food vs. fuel
non-food, abundant bio-waste (agricultural residue, biomass, etc.)
bioethanol, biodiesel, lignocellulosic ethanol, butanol, etc.
environmentally friendly, not competing with food
LPG, Diesel, Petrol, Kerosene, etc.
Petrol stock declining, pollution, economics & ecological issues (global warming)
1 gram of dried durian seed
49 ml RO water
Enzyme variation:
T 60U + A 12U
T 60U + A 36U
T 180U + A 12U
T 180U + A 36U
T 60U --> 20 microlitre
T 180U --> 60 microlitre
A 12U --> 40 microlitre
A 36U --> 120 microlitre
samples were taken at:
30', 60', 90', 120'
DNS method test
3,4-dinitrosalicylic acid
Hydrolysis results
source: meih.st.gov.my
Project Objectives
To obtain sugar monomer (glucose) content through enzymatic hydrolysis
To obtain ethanol from durian seed waste extraction after fermentation (ability of durian seed to produce bioethanol)
To test energy level produced as the final product of bioethanol production from durian seed waste
0.1 gram
0.04 gram
0.02 gram
samples were taken every 3 hours, in 24 hours
Problems Encountered
Raw material was rotten due to improper storage and drying.
Enzyme was acquired late.
Shortage of DNS reagent provided.
Few equipments were brokedown.
Immediate Project Plans
To read the ethanol content of fermentation result.
To test the energy level of the final product.
Full transcript