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PCR

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by

Brayden Gruber

on 1 February 2013

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Transcript of PCR

PCR What is PCR? PCR, or Polymerase Chain Reaction, was invented by Dr. Kary Mullis in 1983. PCR is a very important tool in science because it allows us to make millions of copies of specific DNA in only a few hours. How does it work? 4. Repeat cycle. The themocycler was invented in 1986, making PCR a much easier process. Being able to rapidly change the temperature, the thermocycler makes the PCR process only about 4 hours. WHY? The purpose of PCR is to make multiple strands of a specific sequence from DNA. After only 30 cycles, billions of copies of the target sequence is made. That is all! 1. The DNA is heated to
separate the strands. 2. Temperature is cooled
and primers attach to the
target sequence of the DNA. 3. The enzyme Taq polymerase
then attaches to the target strand
and copies the target sequence. 4. Repeat
Full transcript