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Nanostructured lipid carriers loaded with CoQ10

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huang yung chi

on 8 January 2014

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Transcript of Nanostructured lipid carriers loaded with CoQ10

Nanostructured lipid carriers loaded with CoQ10


*Intracellular reactive oxygen species (ROS)

*Oxidative DNA damage

*Mitochondrial functionality in human dermal fibroblasts (HDF)

Result :

*NLC alone, whether exposed or not exposed to UVA

*Decrease in cell viability associated with enhanced oxidative stress

*Did not lead to oxidative DNA damage nor mitochondrial impairment.

Reduced CoQ10-NLC
*Reduced CoQ10-NLC,differently from oxidized CoQ10-NLC

*Counteract UVA-associated mitochondrial depolarization

*Suggesting a potential role of this molecule in antiageing cosmetological formulations
SLN-The first generation
*High biocompatibility、good physical stability

*Possibility of controlled release of drug and active substances

*Large scale production

*Cheap raw materials

Nanostructured lipid carriers (NLC)
*Drug delivery
*Promote increased bioavailability
*Epithelial penetration of lipophilic compounds

*Cytotoxicity(especially under in vivo usage conditions)

bioactive compounds
*Biological activities (antioxidants, anti inflammatory, enzymatic cofactors)


*Cosmeceutical preparations

*Lipophilic compounds such as tocotrienols, tocopherols, carotenoids, polyphenols, quinones and sterols


*Drug expulsion phenomena

*Particles concentration in the aqueous dispersion ranging from about 1% to a maximum of only 30%

*Insufficient total drug payload

*Combine the advantage of colloidal drug carrier systems,but the drawbacks are avioded or minimized

*Blend of solid and liquid lipid

*Minimizing drug expulsion phenomena

*Increasing drug loading

*Promoting the bioavailability

*The dermal delivery of CoQ10

NLC-the second generation
The Feature of CoQ10
*The fundamental role in mitochondrial bioenergetics

*Well-acknowledged antioxidant properties

*Increase production of basal membrane components

*Fibroblast proliferation

*Protect cells against oxidative damage.

Clinical Applications:
*Cardiovascular disease

*Neuromuscular degenerative diseases


Cosmetic Applications:
*Reduce photoaging in vivo with a corresponding decrease in wrinkle depth

Particle size distribution and zeta potential measurements
Cell viability assay

Cell cultures
NLC preparation
1. Precirol ◎ATO 5(5.1g) was melted at 75°C + Miglyol◎812(2.25g) + CoQ10(500g)

2.Dispersed in the hot(75°C) surfactant solution(Lutrol ©F68,4% w/w and Tween 80,3% w/w)and use a high-speed stirrer at 8000 rpm.

3.Use a UP 400 S and maintaining the temperature at least 5°C.

4.Cooled in ice bath and dispersed in cold water(400ml) under high-speed homogenization at 8000 rpm for 5 min.

*Precirol ©ATO 5


*Lutrol ©F68

*Tween 80

*Reduced and oxidized CoQ10

CoenzymeQ10 supplementation
UVA exposure
Comet Assay
Cell Viability Assay
Intracellular ROS assay
Preparation and characterization of CoQ10ox/red-NLC
Mitochondrial membrane potential assay
Intracellular ROS assay
Comet assay
PCS analyses
This test evaluates mitochondrial function as a measurement of cell viability, since intracellular dehydrogenases located within the mitochondria of only viable cells are able to reduce MTT leading to the formation of purple formazan crystals.

In order to determine the possible genotoxic effects of NLC, DNA damage in terms of double strand breaks (DSBs), single strand breaks (SSBs) and oxidized purine bases was studied.





Mitochondrial membrane potential assay
International Journal of Pharmaceutics 455 (2013) 348– 356
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