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Microbiology: The Final Frontier

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Amanda Veer

on 12 October 2011

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Transcript of Microbiology: The Final Frontier

The Search for the Unknown: Materials & Methods Aha! Results Introduction Last step Background
(cc) image by nuonsolarteam on Flickr Staphylococcus Aureus Gram-positive

Catalase-positive

Facultative anaerobe

Cocci Responsible for most staph infections

Some cases of food-poisoning
Hospital outbreaks
Boils, lesions, skin infections
Septicemia

MRSA (Methicillin-resistant)
VRSA (Vancomycin-resistant) “My delight may be conceived when there were revealed to me beautiful tangles, tufts
and chains of round organisms in great numbers, which stood out clear and distinct
among the pus cells and debris...”
Alexander Ogston (1844-1929) Lives on skin & in nose -

About 20% of the population are always colonized with S. aureus, 60% are intermittent carriers, and 20% never carry the organism (Foster 2004). Amanda Veer 10/11/11 1880 Alexander Ogston (1844-1929)
Joseph Lister (1827-1912) Lac/Dex/Suc broth

Catalase Activity

Gram stain Injected guinea pigs, mice & hen eggs
with pus from infected patients In 1882 Ogston named the clustered micrococci "staphylococci" from the Greek word staphyle, meaning bunch of grapes. What's in a name? Lactose, Dextrose, and Sucrose:

All indicated that Unknown #1
is a fermenter that produces
acid, but no gas Unknown #1 was observed as being catalase positive, being capable of producing catalase and degrade hydrogen peroxide Unknown #1 Gram Stain:

Gram +
Cocci
Grouping:
Single
Chains
Irregular clusters Conclusion:
Unk#1 is most likely Staphylococcus aureus A gram posivite cocci, catalase positive, acid fermenting, facultative anaerobe Resources:

Foster TJ, The Staphylococcus aureus “superbug”, Dec 15
2004, Journal of Clinical Investigation
2004;114(12):1693–1696. doi:10.1172/JCI23825.
Orenstein A, The Discovery and Naming of Staphylococcus
aureus, 2006, Reprinted from www.antimicrobe.org
Turnidge J, Rao N, Chang FY, Fowler VG, Kellie SM, Arnold S,
Lee BY, Tristan A, Staphylococcus aureus,

Dec 2008, Reprinted from www.antimicrobe.org

http://www.clfs.umd.edu/cbmg/faculty/asmith/smith2.html

http://www.jci.org/articles/view/23825

http://www.antimicrobe.org/sample_staphylococcus.asp
Staphylo: bunched like grapes
coccus: any spherical bacteria
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