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Inheritence of Anthocyanin Gene in B. Rapa

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by

Jake Kaminski

on 10 April 2014

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Transcript of Inheritence of Anthocyanin Gene in B. Rapa

Materials
Seeds of designated phenotypes
Seed-collecting pan
Small envelopes
Wicks
Label Tape (Sharpies in our case)
Styrofoam "quads" (used to germinate seeds)
Fluorescent light bank
Water reservoir for petri dish
Water and dropper
Potting mix (soil)
Fertilizer Pellets
Watering Tray
Petri dish with filter paper
Bees or bee-wand
Introduction
Purpose and Rationale
Our experiment was designed to use the Mendelian Model to describe the mode of inheritance in Brassica rapa (Fast Plants) plants with different phenotypes.
We hypothesized that the allele for purple (dominant) and green (recessive) would follow the mendelian rules of inheritence.
Based on this, If the experiment follows the Model of Mendelian Genetics, then the phenotypes of Fast Plants should not be equal.
Rationally speaking, a heterozygous cross should yield a 3:1 ratio.
Methods: Preparing and Planting Seeds
Preparation of Styrofoam Quads
Results
Table 1: Phenotypes of F1 Generation from a Cross of Homozygous Dominant for Anthocyanin Gene and Homozygous Recessive (Lacks Anthocyanin) B. Rapa Plants

Number of Plants
Team No. Purple Green
Our Group 2 1
Class Total 15 6





Table 2: Phenotypes of F2 Generation from a Cross of B. Rapa Plants Heterozygous for Anthocyanin Gene

Number of Plants
Team No. Purple Green
Thursday Afternoon Lab 0 0
Class Total 6 6


Pheno Geno O E (O-E) (O-E)2 (O-E)2/E
Purple ALN/? 6 9 -3 9 1
Green aln/aln 6 3 3 9 3

X2=4
0.05>p>0.025
We should reject our hypothesis because the X2 gives us a probablity that is below 5%.

Discussion
The hypothesis was proven false by the results. There were equal numbers of purple and green plants which indicates the Mendelian model was not applicable. The chi square value was below 5% which proves our hypothesis false.

These results were very unexpected because the cross of two monohybrid plants should generate three purple plants and one green plants. The first generation of plants shouldn't have any green phenotypes because the seeds are monohybrids. This means the dominant trait (purple) should be the only observed phenotype in the first generation.

The results seem to be skewed due to numerous sources of error. One source of error was the inability to discern the actual phenotype of the plants. Another source of error is one of the group members is colorblind which may yield odd results.

By: Zachary Cravener, Sean Daniels,Jacob Colin Kaminski, and Chaz Porter .


Inheritance of Anthocyanin Gene in
Brassica Rapa Fast Plants

Place the absorbant wicks in each of the four holes within each quad; the tips must extend outwards.
Place potting soil in each quad, followed by fertilizer pellets, and then cover each pellet with soil. Make a small depression and plant two to three seeds in each cell; cover with a small amount of soil.
Water each seed with a dropper until the wicks are all dripping. Place on a watering tray, dish, or mat for collection of excess water.
Four to five days later, record the phenotypes of each plant, and keep only the healthiest seedling in each quad.
Prepare the second generation by cross pollinating two plants in the first generation using the bee stick.
Repeat the previous steps for the second generation and record the phenotypes.

Anthocyanin is a purple pigment found
in a variety of plant species, including
Brassic Rapa
Wisconsin Fast
Plants™. Anthocyanin is generally best observed within first week of growth on the stems and leaf tips. A single gene, one of 300, anl, regulates expression in anthocyaninless plants; no purple pigment is observed in the phenotype itself.
regulates whether or not anthocyanin will be
expressed. In the homozygous, recessive form
(anl/anl), anthocyanin expression is completely
suppressed, and the plants appear a bright
green color (which is the “non-purple stem ”
phenotype). If the genotype is anl/ANL or ANL/
ANL, then anthocyanin is expressed at
varying levels and the plants are the “purple
stem” phenotype. The genotype of the wild
type is ANL/ANL.
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