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Denaturing Proteins: Do all proteins denature at the same temperature

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Ethan Laurenceau

on 4 January 2013

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Transcript of Denaturing Proteins: Do all proteins denature at the same temperature

Brainstorming Results Realisation Sketches The results from my project indicate that hair which is keratin protein denatured at the highest temperature. From these results it is obvious that the hypothesis is proved to be correct. The procedure had steps on how to test if the proteins denatured at the same temperature. The procedure for this experiment mostly succeeded because it proved my hypothesis right away, but it also had a negative effect because it didn’t have a control variable in my experiment. There should be changes in the experimental procedure such as a control variable should be included to the experimental procedure. Conclusion Small saucepan
6 eggs (any size)
2 mixing bowl
Candy thermometer
Two quarts of powdered Milk
Cookie Sheet
Aluminum foil
Hair from a hairbrush/come
Toaster/Conventional oven Materials If all proteins were put in the same temperature then they would not denature because of their different structure and characteristics. Hypothesis Do all proteins denature at the same temperature? Question We will be able to take a journey and figure out how the process of denaturation is used in our lives every day. For example cooking, baking, and grilling are all ways to denature proteins. This project is important because it can be beneficial when used in activities such as cooking. The problem was do all proteins denature at the same temperature, so the purpose of this project is to determine whether all proteins denature at the same temperature. The hypothesis was that if all proteins were put in the same temperature then they would not all denature, because the proteins have different structures. Introduction "Animation: Protein Denaturation." Animation: Protein Denaturation. N.p., 2007. Web. 04 Dec. 2012. <http://highered.mcgraw-hill.com/sites/0072943696/student_view0/chapter2/animation__protein_denaturation.html>.
"MLA Style Page Format." MLA Style Page Format. N.p., 20 Aug. 1998. Web. 04 Dec. 2012. <http://www.csus.edu/owl/index/mla/mla_format.htm>.
Webb, Ashley. "Denaturing Proteins." Education.com. N.p., 2006. Web. 04 Dec. 2012. <http://www.education.com/science-fair/article/denaturing-proteins/> Works Cited Results/Graphs Figure 1- Protein Denaturation Temps (Trial 1) Results/Graphs Table 2-Protein Denaturation Temp (Fahrenheit) Results (cont.) Table 1-Protein Denaturation Temp (Celsius) Results Crack an egg over the first bowl
Use an empty water bottle as a suction device. Place the rim of the empty water bottle over the yolk. Squeeze the bottle and release. The yolk should get sucked up in the bottle leaving white in the bowl.
Use two bowls, keeping all the whites in one of the bowls. Make sure that your yolks do not contaminate the whites.
Transfer the whites into a small saucepan. Place the candy thermometer into the saucepan.
Gently heat the whites. Record the temperature when their texture changes.
Clean the saucepan, thermometer, and bowls.
Make two cups of reconstituted powdered milk according to the package directions and add to the saucepan. Place the candy thermometer in the saucepan.
Gently heat the milk. Record what temperature the texture of the milk changes or a skim forms over the top.
Cover a cookie sheet with aluminum foil.
Preheat the oven for ten minutes to 200 degrees.
Using a comb, pull all the hair out of a hairbrush and put on the cookie sheet.
Put the cookie sheet and hair into the oven. Let it heat up for 15 minutes. Inspect the hair for any changes.
Increase the temperature by 25 degrees. After 15 minutes, inspect the hair again.
Keep increasing the temperature in 25-degree increments. Note when the hair texture changes. Procedure Ethan Laurenceau
Harvard,8th Grade
Ms. Williams
Jan 2, 2012 Do all proteins denature at the same temperature Denaturing Proteins Figure 2- Protein Denaturation Temps (Trial 2) Results/Graphs
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