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Screening and Bioassay of CNS acting drugs

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by

asmaa ali

on 11 March 2015

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Transcript of Screening and Bioassay of CNS acting drugs

Thank You!
Hole Board Test
1- clean the board effectively
2- we have two mice:
Calculations:
% of control:
Open Field Test
Procedure:
we have two mice:
procedure:
hole board
open field

Screening and Bioassay of CNS acting drugs
we have to know:
1- Exploration:
first nose poking in the hole.
2- Repetition:
the second repeated nose poking in the same hole without changing the body position or leaving to another hole.
3- Habituation:
simple form of learning, in which an animal, after a period of exposure to stimulus, stop responding.

the principle:
evaluation of CNS activity of the drug ( stimulant or depressant ) through two parameters:


1- exploration
2- repetition

CNS stimulant drug will increase these parameters, and CNS depressant drug will decrease these parameters.
1
2
- put the mouse on the board

observe for 5 min. (
first trial
)
remove and wait for 15 min.

observe for 5 min. (
second trial
)
remove and wait for 15 min.

observe for 5 min. (
third trial
)
the control mouse:
- inject the drug I.P. (dose = weight)
- wait for 15 min.

- put the mouse on the board

observe for 5 min. (
1st trial
)
remove it, wait for 15 min.

observe for 5 min. (
2nd trial
)
remove it, wait for 15 min.

observe for 5 min. (
3rd trial
)
Tabulate your results.
no. of expl. or repet. for a drug in trial
no. of expl. or repet. for the control in trial
X 100
Plot your results:
We need to know:
1- Locomotion:
no. of squares, crossed by the mouse.
2- Rearing:
standing on the lower hind limbs
without support.
3- Grooming
The Principle
evaluation of CNS activity of the drug ( stimulant or depressant ) through these parameters:

1- Locomotion
2- Rearing
3- Grooming

CNS stimulant will increase these parameters, and CNS depressant will decrease these parameters.
1
2
1- control
- clean the board

- put the mouse at the
center
of the field.

- observe for
15 min
. divided into
5
intervals,
3 min
each.

- count the
no.
of locomotion, rearing, and grooming.
2- The Drug:
inject the mouse I.P.
( dose = weight )
wait 15 min.

transfer the mouse to the
center
of the field.
observe for
15
min, divided into
5
intervals,
3
min. each.

count the no. of locomotion, rearing, and grooming.
Tabulate your results:
non-cumulative count
Plot your results:
face washing and scratching done by rat's fore legs, licking of the fur and genitals.
Full transcript