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Osmosis Egg Lab
Transcript of Osmosis Egg Lab
Data and Graph
Thank you for your attention!
How can we observe osmosis across a semi-permeable membrane?
Osmosis is the movement of water from an area of high concentration to an area of low concentration. A hypertonic solution has a greater concentration of solutes outside the cell than inside the cell. A hypotonic solution has a greater concentration of solutes inside the cell than outside the cell. In an isotonic solution, the concentration of solutes is equal both inside and outside the cell. An animal cell in a hypertonic solution will shrink and shrivel up, because water will be moving out of the cell (area of high concentration) to the solution (area of low concentration). An animal cell in a hypotoic solution will expand in size, because water will be moving into the cell. In our experiment, vinegar is isotonic, light corn syrup is hypertonic, and tap water is hypotonic.
If we put the egg in a hypertonic solution (light corn syrup), then the egg will shrink and shrivel up.
If we put the egg in a hypotonic solution (tap water), then the egg will expand.
Results in line graph
1 chicken egg (soaked in vinegar)
125 mL of light corn syrup
1 glass jar
100 mL tap water
Osmosis with an Egg Lab
Hypertonic vs Isotonic vs Hypotonic
1. Get a chicken egg that has been soaked in vinegar over a two day weekend.
2. Measure and record the mass of the egg on a scale.
3. Measure and record the circumfrence of the egg using a piece of yarn and a ruler.
4. Put the egg in a glass beaker and pour light corn syrup into the jar until the egg is fully covered(125mL). Wait a day (24 hours)
5. Measure the circumfrence and mass of the egg. Record.
6.Lightly wash off the corn syrup from the egg & beaker.
7. Pour 100mL of tap water into the beaker and then place the egg into the beaker. Wait a day (24 hours)
8. Pour the tap water out of the beaker
9. Measure and record the mass and circumfrence of the egg.
10. Place the egg back into the beaker.
11. Take a straightened paper clip and gentley pop the egg.
12. Clean the beaker, discard of the egg, and clean your desk.
Results in Table
1. The independent variables in this experiment were the light corn syrup and tap water(solutions). The dependent variables are the mass and circumfrence of the egg.
2. The control was the egg originally soaked in vinegar. We needed it so we could compare the mass and circumfrence of the egg.
3. The egg swelled in the hypotonic solution because water was moving into the cell.
4. The egg shrunk in the hypertonic solution because water moved out of the egg.
5. The results supported our hypotheses because the egg shrunk (-20% circumfrence, -39% mass) and swelled (+18.8% circumfrence, +41.4% mass) just as we predicted.
6. Osmosis is the process of water moving from an area of high concentration to an area of low concentration. It is passive transport.
7. Passive transport doesn't require energy; active transport does. Passive transport includes osmosis and diffusion while active transport includes endocytosis and exocytosis. Active transport uses protein pumps to enter the cell.
Both of our hypotheses were correct. In hypothesis A, we predicted that the egg would shrink in the hypertonic solution. We were correct because the circumfrence decreased by 20 percent and the mass decreased by 39 percent. In hypothesis B, we predicted that the egg would swell in the hypotonic solution. We were correct because the circumfence grew by 18.8 percent and the mass grew by 41.4 percent.
Possible sources of error: We could have put it in the hypotonic solution first, making it swell first. We could have handled it roughly and affected its ability to absorb water.
"Answer Key." Review Guide: Osmosis and Diffusion. N.p., n.d. Web. 04 Oct. 2013.
Gatlin, Johnny. Osmosis. Honors Biology. Panther Creek High School.23 Sept. 2013