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Tere MexCity SPAN/ENG 2016 09

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Raymond McCauley

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Transcript of Tere MexCity SPAN/ENG 2016 09

TALENs
Reading DNA
+1-650-776-4561
for your attention
Raymond McCauley
biochemistry
biophysics
electrical eng.
computer science
bioinformatics
nanotechnology
genetic engineering
DNA sequencing
personalized health
DISCLAIMER
These are my opinions and are not intended to reflect the views of any group or organization with which I may be affiliated.
Exponential Biosciences
systems
biology
past:
Biotech Chair: Singularity U
Co-Founder: Playtpus Project
Co-Founder & Chief Architect: BioCurious
Principal: Exponential Biosciences

Current Research Areas:
Personal Genomics
Therapeutic Genetic Engineering
Low Cost Access to Biotech Tools
Cancer Dx and Treatment
Singularity
University
Raymond McCauley
@raymondmccauley
Thank you
Raymond McCauley
DNA and the
next battle against cancer
raymond@singularityu.org
+1-650-776-4561
Conflicts
Medicine
Agriculture
Energy & Manufacturing
Biotechnology =
1/3 of Global Economy
& Financial Interests
Anthrocyte
Deloitte
Exogen
Genomera
GLG
Illumina
Intellimed
Platypus Project
QIAGEN
Singularity University
Verinata
WellPoint
Marked with throughout presentation.
*
Caterpillar
Disruption
is never
Expected
Food
example: Okanagan Fruit Specialties
Not multinational, but family startup
Not implanted, but adjusted
Not labeled, but branded
Cocoa
$2.1B
Chocolate
$131.7B
by 2019...
shifting production
global warming, disease
genetic engineering
shifting consumption
Asian markets
production innovation
health benefits
source:
Cocoa & Chocolate Market - Global Trends & Forecasts to 2019.
Entire presentation copyright 2014 by Raymond McCauley
Disruption
Mother (Nature)
Biotech
or
is the
of all
2020
=
+
2001
=
ApE = A Plasmid Editor
Rise of the
Biotech
Garage
Inventor
bioprinter project
OpenPCR
$599 DNA Copy Machine
+
Industrial
Biology
Biohacking
Cow-Free
Milk & Cheese
Genetic
Engineering
Software Development
=
The
small
future
is
industrial microbiology
yeast = tiny factories
like microbreweries
gene prospecting
supply chain disruption
Microbiomes
Bacterial Reservoir
Eat ammonia
Eliminate smell
Crowd out
tinea pedis
Keep feet healthy
Peter DiLaura, CEO
Practical Advice
Use antibiotics wisely
Eat like ancestors
Get a dog
Citizen Science
& Crowd-Sourced
Metagenomics
Microbiomes
Wearables/
Implantables
Microbiome Roles
Digest food
Synthesize nutrients
Protect against disease
Mood, sleep, eating behavior
Partnership with Mayo Clinic, as of Oct 2014
inflammatory bowel diseases
metabolic disorders
colorectal cancer

Secret Clinical Trials, revealed Feb 2015
1. Avoid excess sugary foods
2. Good quality sleep
3. Meditation and relaxation
4. Eat fiber
5. Eat natural probiotic foods
6. Consider probiotic supplements
The Gut Microbiome,
Anxiety and Depression:
6 Steps to Take
by Peter Bongiorno, ND, LAc
November 8, 2014
Jessica
Richman,
CEO
microbiome hacking
& DIY fecal transplants
Phage
Therapy
Use viruses to kill bacteria
Specifically targeted
Alternative to wide spectrum antibiotics
Developed in Russia, new Western interest
Image credit: Bacteriophages and Biotechnology
http://www.pharmainfo.net/luckypharmacist/bacteriophages
http://rockhealth.com/tag/wearables/
now
next
wearables
implantables
We are
all
cyborgs
vaccination
biotechnological
artifact
xNTi kit
RFID chipset
2x12mm cylindrical USP grade lead-free Schott 8625 biocompatible glass casing
Pre-loaded injection assembly
Use with 13.56MHz RFID systems & NFC

$99 from
dangerousthings.com
to show
implants are
easy
Shaping the oral microbiota
through intimate kissing
Remco Kort, Martien Caspers, Astrid van de Graaf, Wim van Egmond, Bart Keijser and Guus Roeselers
...we investigated the effects of intimate kissing on the oral microbiota of 21 couples by self-administered questionnaires about their past kissing behavior and by the evaluation of tongue and salivary microbiota samples in a controlled kissing experiment...
"
"
brings up many questions...
RFID
Hackers:
Learn by
Doing
2015
=
App: NFC TagInfo
Read chip, unlock phone, open websites
Open (some) hotel doors
What can I do?
Access (a few) credit card machines
and
biosensing
and
command & control
are
coming soon
but mainly, I get a lot of funny looks
One More Thing...
from being in
an exponential business
Find an exponential trend,
then hitchhike
A prototype beats a plan
Product Development Process (PDP)
typical product:
18 month development cycle,
9 month market life

make new & rev old products
core team leaders involved from start
phase-gated milestones
manage technical and business risk
Nothing fades faster than last year's technology
2008
$500K
2012
$1
People
Teams
Interdisciplinary
Disruption
is never
Expected
Salim Ismail
Add a specialty
Be half of a team
Assemble a varied team
Find translators
Develop internal people
Innovation Lessons
Triple Major?
Design Your Own Major?
A hackerspace for biotech

First biohacker lab in the world

Committed to making biotech
accessible, affordable, and open to everyone

Located in Sunnyvale, California

501c3 all-volunteer community lab, supported by monthly memberships and classes
BioCurious/FBI
Joint Conference on
Biohacking
June 12-16, 2012
Makes vaccines using mRNA from diseases
"Vaccinated with information"
Gates Foundation invests $57M
"Cellular scissors chop up HIV virus"
Salk Institute
Nature Communications "Use of the CRISPR/​Cas9 system as an intracellular defense against HIV-1 infection in human cells", Liao et al
How long until
genetic engineering
for
humans
?
ZFPs
TALENs
CRISPR/Cas9
$10000
$5000
$1000
Better, Cheaper, Faster
Genetic Engineering
*
The
future
is
coming
fast
2010: discovered
2011: microbes
2012: plants, animals
2013: human cells, company
2014: patent fight
Drag and Drop
Genetic Engineering
Transcription
Activator
Like
Effector
Nucleases
TALENs have modified genes in:
yeast
fruitfly
roundworm
cricket
zebrafish
frog
rat
pig
cow
thale cress
rice
silkworm
human somatic cells
pluripotent stem cells
Nature Reviews Molecular Cell Biology 14, 49-55 (January 2013)
http://www.brc.a-star.edu.sg/index.php?sectionID=12
hijacked from plant bacteria that genetically engineer their host plants
discovered by Bogdanove & Moscou, Iowa State University, in 2009
make double stranded breaks to knock-out and knock-in genes
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
extraído del mecanismo del sistema inmunológico procariótico
descubierto por Horvath y Barrangou, DuPont/Danisco, en 2010
inmunizar bacterias industriales
silenciamiento génico en células madre
CRISPRs
MSTN
LRP5
APP
CCR5
PCSK9
FUT2
SLC30A8
Lean muscles
Extra-strong bones
Alzheimer’s-resistant/immune
HIV-resistant/immune
Lower coronary risk
Stomach flu resistant
Type 2 Diabetes
Genes to Knock Out
adapted from George Church, private communication
Behavioral and Mental Traits
MAOA gene & aggression
last to implement
poorly understood
Therapeutics,
then
Enhancement
Gene Expression
"gene doping"
EPO, red blood cell production
Myostatin, muscle growth inhibition
VEGF, blood vessel production
IGF-1, targeted muscle mass increase
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali, Luhan Yang, Kevin M. Esvelt, John Aach, Marc Guell, James E. DiCarlo, Julie E. Norville, and George M. Church
Science 15 February 2013

Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong, F. Ann Ran, David Cox, Shuailiang Lin, Robert Barretto, Naomi Habib, Patrick D. Hsu, Xuebing Wu, Wenyan Jiang, Luciano A. Marraffini, and Feng Zhang
Science 15 February 2013
Academically (for reference)...
Commerically...
$43 million Series A , Nov 2013
Flagship Ventures
Polaris Partners
Third Rock Ventures
Partners Innovation Fund
TALENs
CRISPRs
CRISPR/Cas9
is a toolkit for
Genes
and regulatory regions
Knock-out
Knock-in
Repair
Edit
cheap
highly targeted
improvable
bluebird bio
Editas Medicine
GenSight
Lysogene
Spark
uniQure BV
Voyager Therapeutics
and others
Genetic Engineering
Therapeutics
Companies
they work,
everywhere
Human
Infertility
Prenatal Genetic Dx
Mitochondrial Therapy
Germ-Line Therapy
Enhancement
- Disease Risk
+ Intelligence
+ Longevity
NOW
NEXT
genetic
engineering
23andMe and Apple
enter drug research business
"crowd-sourced clinical trials"
Apple ResearchKit
- Asthma
- Parkinson's Disease
- Breast Cancer
- Diabetes
- Cardiovascular
Enrolled 7400+ people in 24 hours.
Previous largest study was 1700.
Why did I do it?
"CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes"
Puping Liang, et al, Guangdong Province Key Laboratory of Reproductive Medicine
Announced April 2015
Attempted Beta-Thalassemia therapy
Used nonviable embryos
Found it hard to do - off target & chimerism
Too controversial for most journals
Announced First Human Embryos Edited by CRIPSR
Biology now falls under Moore's Law
DNA Sequencing Economics
Cost
DNA Sequencing Tech
3X ~ 5X

Computer Tech
1.5X ~ 2X
Your chance to try it...
Consumer access to research tool
"Recreational genomics"
Disease risk, carrier status, genealogy
DNA Sequencing
2018
2022
=
=
=
Source: National Human Genome Research Institute, Exponential Biosciences
Every Disease
is a
Rare Disease...
marginal cost,
market segmentation
& value-added services
1 human genome + interpretation
=
$3900

5 human genomes, raw data, in bulk
=
$3000

1 fast delivery human genome
= $5000

1 human exome
=
$400

1 point-of-care bacterial identification
= $20 - $100+

1 human "gene scan" (e.g., 23andMe)
=
$99
Illumina Custom OmniExpress+ BeadChip
Assays about 1,200,000 oligo sequences
DNA Scan
DNA Sequence
most interesting/best known
1.2 million bases
0.04%
almost all
3.2 billion bases
100%
DNA Microarrays
Tools
What can you do about
being at risk for
an
unpreventable
disease?!?
It turns out, lots of things:

prevention - vitamins, avoid UV
testing - Amsler grid
new opthamologist
investigate clinical trials
support research
don't put off that trip to the Louvre...
Try it...
So What?
Two
Personal
Examples
When I first found out about
my AMD risk
it was
"
untreatable
" & "
incurable
"
but
Genentech

now has 2 treatments available
footnote
*
Rare Disease
is the
Long Tail
of human health...
2013
=
2007
=

Covers
Disease risk, carrier status, genealogy

What do
you
want to know?
Some of your info also applies to
family...

Privacy:
to share or not to share?
Do you want to be found by long-lost relatives?
In US,
Health insurance
protected by law

(GINA)
Life insurance, long-term disability is
not
Next Generation Sequencing
Next
14 Jan 2014
HiSeqX 10
$10M
20 000 genomes/year
$1000/genome, all in
NextSeq 500
$250K
1-10 genomes/day
Now
Reactive
Near Future
Medicine
How does it work?
What do you get?
Other applications
NIPT = Non-Invasive Prenatal Test
Has replaced Amniocentesis
Tests for chromosomal disorders
Finding Fetal DNA in blood: Data Problem
Biggest Drug Problem?
compliance
+
personalization
Rohan Coelho, Rexanto
packaging
&
sensors
NASDAQ: ILMN
Market Cap: 27.26 B
P/E: 66.42
dominates market
virtual monopoly
large cash reserves
purchase/license new dev

*
=
Predictive
The End of
Mammograms
Colonoscopies
y
circa 2018-2020
*
2015
=
"What kind of infection?"
DNA Sequencing
Reading DNA
Genetic Engineering
Writing DNA
Hacking DNA
Democratization
Access to Tools
Pharmacogenomics
More Accurate - Less Risk - Fraction of the Cost
Test Types
Throat swab culture
DNA probe
RADT - Optical Immunoassay
RADT - Enzyme Immunoassay
Rapid qPCR
Time

48 hours
2 hours
<1 hour
<1 hour
<1 hour
Special Equip
-
$$
$$$
-
$
blood sugar
blood chemistry
temperature, blood pressure, ECG
transplant rejection
heat attack, stroke early warning
untreatable
incurable
You can't buy ENIAC for a penny

But you
can
buy:
Decent Laptop, $800
An 100 hours of cloud computing, $130
Graphics workstation, $11000
Moore's Law
Decent Laptop, $800
An 100 hours of cloud computing, $130
Graphics workstation, $11000
BETTER
circa 2010
circa 2015
Today:
$200
qPCR
with
20 minute
prep & run time
Stealth companies that contacted me
advisor,
consultant,
investor
Do you
believe?
Synthetic Biology Explained
James Hudson/Bridge 8
Video
6 min 27 sec (partial)
Control of
cell differentiation
& growth
synthetic biology / 3D bioprinting
tissue engineering / cultured meat / food
environmental impact
food security
Making Leather and Meat Better
over-hyped
poor marketing
artificiality
synthetic = inferior
Biology
Synthetic
Deliver genetic medicines
Water purification
Food preservation
We are
everywhere
...
currently tracking
23 community labs
200+ individual labs
on 6 continents
50+ companies out of BioCurious
$129+ Million in follow-on funding
Startups & schools copying model
Worldwide biohackerspaces
Rise of the
Biotech
Garage
Inventor
most innovative
education company
in the world
Boston
Paris
San Diego
Seattle
Oakland
Portland
Manchester UK
Biologigaragen
Biospace
Victoria, Canada
Copenhagen
Switzerland
Brooklyn
Sao Paolo
San Francisco
Shenzhen
Tokyo
Bio
Garage
Baltimore
Syn
Bio
Brasil
Top 100
#4
We teach
is
old
and
new
Agriculture
=
Biotechnology
Makerspaces
as an educational model
leverage existing schools, industries
versus video-based internet education
retention
offshorability
interdisciplinary & soft skills
Medicine
most disrupted
industry
profession
institution
business model
information
assymetry
flips
short term
people live longer
chronic care costs higher
Hospitals
in current form
= old model
long term
people live longer
health costs drop
biotechs
versus
pharma
small
innovators
job creators
research, agility, talent +
large
incrementalists
force reduction
+ finance, sales, regulatory
supercentralized
decentralized
subsidized
concierge care, Uber for healthcare
specialization, med tourism
still need emergency rooms, chronic care
=
eldercare
trends
wearables & implantables
sensors
robotics
patient activism
crowd-sourced clinical trials
medium-term: robotics & telemedicine
biotechs
versus
pharma
small
innovators
job creators
research, agility, talent +
large
incrementalists
force reduction
+ finance, sales, regulatory
+
2001
=
+
+
Trends: Eco Foods, Food Security 3.0
Livestock Genotyping
trait prediction
breed, pedigree, parentage
identification
outbreak traceability
feed optimization
Genus PLC
and PIC, UK
BGI,
Shenzhen
Microbiomes
& Metagenomics
Peter DiLaura, CEO
Citizen Science
& Crowd-Sourced
Metagenomics
Microbiome Roles
Digest food
Synthesize nutrients
Protect against disease
Mood, sleep, eating behavior
Partnership with Mayo Clinic, as of Oct 2014
inflammatory bowel diseases
metabolic disorders
colorectal cancer

Secret Clinical Trials, revealed Feb 2015
microbiome hacking
& DIY fecal transplants
New Study: Microbiome & Weight Loss
Pet DNA Testing
Breed Identification
Vet Insurance
Cancer Research
http://www.economist.com/news/science-and-technology/21679436-body-temperature-seems-part-be-controlled-gut-bacteria-cold-weather
http://www.sciencedirect.com/science/article/pii/S0092867415014841
Mice with "cold microbiomes" change insulin sensitivity, calorie absorption, intestine anatomy
genome
blood+saliva
activity+food+sleep
microbiome
co-founder, Lee Hood,
Institute for Systems Biology
Scientific Wellness
"Sub-Clinical"
Executive Health / Concierge Medicine
quarterly check-ins + counseling
$2000/year
Cancer
DNA
Companies
adapted from Eric Topol
Hereditary Breast and Ovarian Cancer Testing
$250
$199
26 genes, 20 countries
19 genes, international
redneck
genetic
engineering
Gene Test
for Fitness
Enthusiasts
15 gene markers, 1500 DKK
Lego
+ DNA Origami
= Nanotechnology
DNA
Origami
Solugen
peroxides
from CO2
with enzymes
20x cheaper
portable
SynBio NewCo
small
The
future
is
Industrial
Microbiology
bacteria
yeast
algae
animal feed
protein production
heterotrophic algae
aquaculture
breeding genomics
breweries & distilleries
entirely GM free
privately held,
revenue doubling
$4B in 2015
Disruption
for
Oil, Gas & Coal?
microorganisms = factories
synthetic biology
industrial biology
industrial chemicals - food - agriculture biofuels - medical research
or
DNA = blueprints
new factories = exponential
global capacity
61.4B gal
per year by 2018
source: Lux Research, Sept 2015
KLM Biofuel Program
Sustainability & Corporate Responsibility
80% CO2 reduction
Expensive
but getting cheaper
2013: 6 X kerosene
2016: 3 X kerosene
Fully sustainable in 2050 (SkyNRG)
& portable
price & supply chain disruption
Feeding farmed salmon
with protein from methane
Herramientas

El ADN y la próxima batalla contra el cáncer
bioinformática
nanotecnología
bioquímica
biofísica
ingeniería eléctrica
ciencias informáticas
pasado:
asesor,
consultor,
inversor
Secuenciación de ADN
Interpretación del ADN
Escritura del ADN
Decodificación del ADN
Ingeniería genética
Democratización, Acceso a herramientas
Interpretación del ADN
Micromatrices de ADN
Secuenciación de ADN
¿Y entonces?
Medicina del
futuro cercano
Predictiva
Reactiva
NIPT = Prueba prenatal no invasiva
Ha reemplazado la amniocentesis
Análisis por trastornos cromosómicos
Búsqueda de ADN fetal en sangre: Problema de datos
Más preciso - Menos riesgo - Fracción del costo
¿El final de las
mamografías
&
colonoscopías
Análisis de cáncer de mamas y ovarios hereditario
19 genes, internacional
26 genes, 20 países
Compañías
de ADN
del cáncer
adaptado de Eric Topol
Prueba genética para apasionados por el deporte
15 marcadores genéticos, 1500 DKK
Prueba de ADN
de mascotas
Identificación de razas
Garantía veterinaria
Investigación del cáncer
Genotipado de ganado bovino
predicción de características
raza, pedigrí, linaje
identificación
trazabilidad de brotes
optimización de la alimentación
Tendencias: Eco Foods, Food Security 3.0
Microbiomas
y metagenómica
Peter DiLaura, director ejecutivo
Funciones del microbioma
Digestión de alimentos
Síntesis de nutrientes
Protección contra enfermedades
Estado de ánimo, sueño, alimenticio
Ciencia cívica
y metagenómica
de colaboración masiva
decodificación del microbioma
y trasplantes fecales
"hágalo usted mismo"
Secuenciación de próxima
próxima
generación
Ingeniería genética
Desarrollo de software
Un editor plásmido
El
futuro
se
acerca
rápidamente
Herramientas
Mejor, más económico, más rápido
Arrastrar y soltar la ingeniería genética
Explicación de la biología sintética
James Hudson/Bridge 8
Video
6 min 27 seg (parcial)
bacterias
hongos
algas
Microbiología industrial
pequeño
biología industrial
o
biología sintética
microorganismos = fábricas
ADN = prototipos
nuevas fábricas = exponenciales
y portátiles
precio e la cadena de suministro interrupción
químicos industriales - alimentos - agricultura - biocombustibles - investigación médica
capacidad global
232,4B L
por año hasta 2018
fuente: Lux Research, sept 2015
Programa de biocombustible de KLM
Sustentabilidad y responsabilidad corporativa
80 % de reducción de CO2
Costoso,
pero cada vez más económico
2013: 6 veces querosene
2016: 3 veces querosene
Completamente sustentable en 2050 (SkyNRG)
peróxidos de CO2 con enzimas
20 veces más económico
portátil
¿Interrupción
petróleo, gas y carbón?
de
Control de diferenciación
y crecimiento celular
Origami de ADN
+
Origami de ADN
=
Nanotecnología
Alimentos
Ejemplo: Especialidades de frutas de Okanagan
No multinacional, pero inicio familiar
No implantado, pero ajustado
No etiquetado, pero con marca
propiedad privada,
duplicación de ingresos
$4000 millones en 2015
alimentación animal
producción de proteínas
algas heterotróficas
acuacultura
genómica de reproducción
cervecerías y destilerías
completamente libre de GM
¿Cuánto tiempo
hasta usar la
ingeniería genética
en
seres humanos
?
Infertilidad
Diagnóstico genético prenatal
Terapia mitocondrial
AHORA
Humana
ingeniería
genética
La terapia de la línea germinal
Mejora
- Riesgo de enfermedad
+ Inteligencia
+ Longevidad
DESPUÉS
Gen MAOA y agresión
último en implementarse
poca comprensión
Características conductuales y mentales
Genes para eliminar
Músculos magros
Huesos extrafuertes
Resistencia/inmunidad al Alzheimer
Resistencia/inmunidad al VIH
Riesgo coronario más bajo
Resistencia a la gripe estomacal
Diabetes tipo 2
Expresión genética
"dopaje genético"
EPO, producción de hematíes
Miostatina, inhibición del crecimiento muscular
VEGF, producción de vasos sanguíneos
IGF-1, incremento de masa muscular orientada
Terapéutica,
luego
mejora
ingeniería
genética
de cateto
Anuncio de primeros embriones humanos editados por CRIPSR
Edición de genes mediados por CRISPR/Cas9 en zigotos tripronucleares humanos
Puping Liang, et al, Guangdong Province Laboratorio clave de medicina reproductiva
Anunciado abril de 2015
Se intentó la terapia para beta talasemia
Se usaron embriones no viables
Se encontraron dificultades: desfase y quimerismo
Demasiado controversial para la mayoría de las publicaciones
Biodecodificación
Un espacio para hackers de biotecnología

Primer laboratorio biohacker en el mundo

Compromiso para que la biotecnología
sea accesible, asequible y pública

Situado en Sunnyvale, California

Laboratorio comunitario con todos voluntarios 501c3, respaldado por clases y membresías mensuales
Dictamos clases...
compañía educativa
más innovadora en el
mundo
Principales 100
Máquina de copia de ADN
proyecto de bioimpresora
Leche y queso
Auge del
inventor de
biotecnología
en el garaje
Más de 50 compañías surgieron de BioCurious
Más de $129 millones en
financiación de seguimiento
Emprendimientos y escuelas copian el modelo
Espacios para hackers en todo el mundo
Conferencia conjunta de
BioCurious/FBI sobre
la biodecodificación
12 a 16 de junio de 2012
Portátiles/
implantables
Gracias
por su atención
Matrices sobre 1.200.000 secuencias de oligonucleótidos
El
futuro
es
Asociación con Mayo Clinic, a partir de oct 2014
enfermedades inflamatorias del intestino
trastornos metabólicos
cáncer colorrectal

Ensayos clínicos secretos, revelados en feb 2015
producción cambiante
calentamiento global, enfermedad
ingeniería genética
consumo cambiante
mercados asiáticos
innovación de producción
beneficios médicos
Cacao
fuente:
Cocoa & Chocolate Market - Global Trends & Forecasts to 2019.
para 2019...
Nuevo estudio: Microbioma y pérdida de peso
Los ratones con "microbiomas fríos" cambian la sensibilidad a la insulina, absorción de calorías, anatomía del intestino
Enfermedades hereditarias
Respuesta a medicamentos
Factores de riesgo genéticos
Rasgos característicos
?
?
14,4 %
de los hot-dogs y las salchichas sometidos a prueba presentaron problemas.
ADN humano en el 2 % de las muestras
2/3
de las muestras de ADN humano eran productos vegetarianos.
10 % de los productos vegetarianos contenían carne.
Encontramos pollo en una salchicha de desayuno vegetariana y cerdo en un hot-dog vegetariano.
de ingeniería genética
Tools
sugarcane
corn
biocombustibles
biofuels
Lego
classes...
no provenientes de la vaca
Auge del
inventor de
biotecnología
en el garaje
Biohacking
Resources
for Kids
bit.ly/kidbiohack
Recursos de
biodecodificación para niños
Almost the End
Time to Check on
Our Experiment
3 Things
Casi terminamos
Momento de poner a prueba nuestro experimento
3 cosas
Raymond McCauley
raymondmccauley
on Google Hangouts
+1-650-776-4561
1
2
3
Age of Discovery
Our Language Experiment
Nuestro experimento con el idioma
raymond@singularityu.org
Send us your feedback
Envíenos sus comentarios
/
translator /
traductor
cporatti@essencetrans.com
www.essencetrans.com
+54 9 11 6973-8968
Cecilia Poratti
Raymond McCauley
raymondmccauley
on Google Hangouts
+1-650-776-4561
raymond@singularityu.org
My presentation
Mi presentación
DNA Sequencing
Reading DNA
Genetic Engineering
Writing DNA
Hacking DNA
Democratization
Access to Tools
Secuenciación de ADN
Interpretación del ADN
Escritura del ADN
Decodificación del ADN
Ingeniería genética
Democratización
Acceso a herramientas
I am a scientist
I experiment
Trying something new:
Two Languages
Old World
New World
Why Digital Biology?
¿Porque biología digital?
Santiago Ramón y Cajal
in Madrid, Spain
Father of modern neuroscience
C. elegans
worm, 1 mm long
has 959 cells
302 are neurons
6393 synapses
OpenWorm.org
Building the world's first digital life form. Open source.
Mundo antiguo
Nuevo mundo
Jorge Soto, co-founder
*
Zika
& gene drives
GMOs
and the sugar belt economy
3.0, environment, sugarbelt, hectarage
1906 Nobel Prize
gusano, 1 mm de largo
tiene 959 células
302 son neuronas
6393 sinápsis
Can digitally model and simulate the worm's nervous system
Puede crear un modelo digital y simular el sistema nervioso del gusano
Soy científico
Hago experimentos
Intento cosas nuevas:
dos idiomas
First clinical study, for gastric cancer, with Hospital Clínico Universidad Católica de Chile
microRNA diagnostics in low resource settings
Targeting emerging economies
Primer estudio clínico, para cáncer gástrico, con Hospital Clínico Universidad Católica de Chile
Diagnóstico de microARN en entornos de bajos recursos
Orientación a economías emergentes
Initial work completed at BioCurious in 5 weeks
Trabajo inicial completado en BioCurious en 5 semanas
Old World
New World
Mundo antiguo
Nuevo mundo
Edad de descubrimiento
De Madrid, España
Padre de la neurociencia moderna
Premio Nobel de 1906
maíz
caña de azúcar
rapidly push
new DNA through
whole population
in the wild
inserta rápidamente
nuevo ADN a toda
la población
mundial
www.facebook.com/BiohackingChile/
En Dec 2015, a

GMOs

3.0
Genetically Modified Organisms
better yield
better nutrition
better digestion
drought tolerance
saline soil tolerance
fungus resistance
virus resisitance
Organismos modificados genéticamente
mejor producción
mejor nutrición
mejor digestión
tolerancia a las sequías
tolerancia al suelo salino
resistencia a los hongos
resistencia a los virus
Creative Commons
Attribution-NonCommercial
4.0 International
Creative Commons
Attribution-NonCommercial
4.0 International
Tecnología de secuenciación de ADN
3 veces ~ 5 veces

Tecnología informática
1,5 veces ~ 2 veces
Hoy, la biología está sometida a la Ley de Mooore
costo marginal,
segmentación del mercado
y servicios de valor agregado
Hoy:
1 genoma humano + identificación
=
$3900

5 genomas humanos, datos sin procesar, en forma masiva
=
$3000

1 genoma humano de entrega rápida
= $5000

1 exoma humano
=
$400

1 identificación bacteriana de punto de atención
= $20 - $100+

1 "análisis de genes" humanos (por ej., 23andMe)
=
$99
TALENs have modified genes in:
yeast
fruitfly
roundworm
cricket
zebrafish
frog
rat
pig
cow
thale cress
rice
silkworm
human somatic cells
pluripotent stem cells
Nature Reviews Molecular Cell Biology 14, 49-55 (January 2013)
Transcripción
Activador
Similar
Efector
Nucleasas
En grupos
Regularmente
Interposicionados
Cortos
Palindrómicos
Repetidos
extraído de bacterias de plantas que diseñan genéticamente sus plantas huésped
descubierto por Bogdanove y Moscou, Iowa State University, en 2009
realiza separaciones de cadenas dobles para eliminar o insertar genes
hijacked prokaryotic immune system mechanism
discovered by Horvath & Barrangou, DuPont/Danisco, in 2010
immunize industrial bacteria
gene silencing in stem cells
Academically (for reference)...
Eliminar
Insertar
Reparar
Editar
es un kit de
herramientas para
Genes
y regiones regulatorias
económico
especialmente orientado
capacidad de mejora
funcionan,
en cualquier lugar
y derivaciones genéticas
cofundador
seguimiento actual de
23 laboratorios comunitarios
Más de 200 laboratorios individuales
en 6 continentes
Creación de la primera forma de vida digital en el mundo. Código abierto.
*
Adapted from Stephen Larson and Matteo Cantarelli
Courtesy of Tim Busbice
Courtesy of OpenWorm
Courtesy of OpenWorm
Courtesy of Bio-Rad
(derivaciones genéticas)
synthetic biology
industrial biology
or
biología industrial
o
biología sintética
microorganisms = factories
microorganismos = fábricas
DNA = blueprints
ADN = prototipos
new factories = exponential
& portable
nuevas fábricas = exponenciales
y portátiles
price & supply chain disruption
precio e la cadena de suministro interrupción
industrial chemicals - food - agriculture biofuels - medical research
químicos industriales - alimentos - agricultura - biocombustibles - investigación médica
Biohacking
Resources
for Kids
bit.ly/kidbiohack
Recursos de
biodecodificación para niños
Raymond McCauley
raymondmccauley
on Google Hangouts
+1-650-776-4561
raymond@singularityu.org
April 2016:
Editing CCR5 genes
for HV immunity
Abril de 2016:
Edición de CCR5 genes
por la inmunidad del VIH
Drag and Drop
Genetic Engineering
Arrastrar y soltar
la ingeniería genética
small
The
future
is
Industrial
Microbiology
bacteria
yeast
algae
bacterias
hongos
algas
Microbiología industrial
pequeño
El
futuro
es
Other Technologies
Otras tecnologías
Sensors
Big Data
Self-Driving Cars
Drones
Macrodatos
EHRs
Systems Biology
Coches de
auto-conducción
Sensores
electrónicas puestas
Matternet
Qui-qui
Zipline
DHL
Amazon & Google
entrega droguería
drugstore delivery
AliveCor & Kardia
HCEs
Biología de sistemas
Systems Biology Program @CNB-CSIC
patient transportation
centers of excellence
disaster response
modular hospitals
transporte de pacientes
centros de excelencia
respuesta al desastre
hospitales modulares
escritorio lab de bioproducción
desktop bioproduction lab
$1699
reprogramar
los microbios
reprogram
microbes

nueva fuente
de innovación
new source
of innovation
Miroculus
microRNA diagnostics
First test - stomach cancer detection
Breakthrough: add new tests with informatics
New IP in digital microfluidics
Starting Series A fundraising
Singularity GSP 2014
buscando un bioinformático
New consumer genomics
Bone Marrow Transplants
Transplantes de médula ósea
Dos Mujeres
Two Women
Liquid Biopsy for Colorectal Cancer
Biopsia líquida para el cáncer colorrectal
Jan McCauley
1940-2014
prezi.com/pzqego3gdfsj
Full transcript