Loading presentation...

Present Remotely

Send the link below via email or IM


Present to your audience

Start remote presentation

  • Invited audience members will follow you as you navigate and present
  • People invited to a presentation do not need a Prezi account
  • This link expires 10 minutes after you close the presentation
  • A maximum of 30 users can follow your presentation
  • Learn more about this feature in our knowledge base article

Do you really want to delete this prezi?

Neither you, nor the coeditors you shared it with will be able to recover it again.


Lab Meeting: new tract

No description

Elisa Margolis

on 23 July 2018

Comments (0)

Please log in to add your comment.

Report abuse

Transcript of Lab Meeting: new tract

Lab Meeting: new focus
Clostridium difficile
Hypotheses for Infant Asymptomatic C. difficile
Lack toxin receptors
Daily infant stool samples to capture time of C. difficile colonization and use CCM to screen for members of microbiome which interact with C. difficile and toxin production
Preliminary Data
Would like to demonstrate that infant microbiome reduces toxin production of C. difficile
while deadly in particular hosts can also reside harmlessly in asymptomatic carriers
4 out of 5 infants will have C. difficile with no apparent symptoms
What are some reasons for a bacteria to be a pathogen in some hosts and commensal in other hosts?
some hosts higher pathogen load
(cholera exposure)
(flu + s pneumo)
bacteria gets into
wrong location
differing immune responses
(vaccine, prior infection)
immune over-response
Not toxigenic/pathogenic strains of C difficile
Not high enough levels (no bloom)
Don't produce enough toxin & immune response is tempered
infant microbiome plays role in both
Hamster infant colonization model to look at immune response impact on pathogenesis
Historic Experiments
Toxin Gene Expression
gnotobiotic mice
C difficile Culture
Infant Stool
Toxin Production
(Toxin ELISA)
Toxin Gene
(qPCR of toxin genes)
Controls ?
toxin alters with temperature, nutrient availability
Preliminary Data
toxin discussion so far has been from host perspective- why does C. diff produce toxin?
increase transmission
competition with other bacteria
how would you test these hypotheses?
competition assays between toxigenic and non-toxigenic in sterile stool (long term survival)

toxins as bacteriocins- inhibition assays with adult microbiome flora
Raw feces, fecal water, constructed community?
need help figuring out experiments
Full transcript