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Activity 5

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by

Hoshime Mori

on 11 July 2013

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Transcript of Activity 5

Activity 5:
Detecting Lipids in Plant Cells

Introduction
Objectives
Prepare thin sections of peanut and coconut endosperm.
Follow procedures in staining and mounting specimens for microscopic study.
Identify or test for the presence of lipids in plant cells using micro-chemical method.
Draw specimens as observed through the microscope.
Compare lipid in peanut and coconut endosperm as observed through the microscope.
Materials
Equipment:
Compound microscope
Petri dishes
Glass slides
Cover Slips
Pincettes
Medicine droppers
Chemicals:
0.5% Sudan III stain - 10 mL
Glycerin - 10 mL
50% ethyl alchohol - 20 mL
Supplies:
Raw peeled peanut (2-5 cm long)
Coconut endosperm - 1/4
Razor blades
Ruler
Strips of filter paper
Tissue paper
Watch
Knife
Procedure:
For Peanut Endosperm
For Coconut Endosperm
Cut 10 paper thin slices (cross-sections) using scalpel
Place out section in a p.d. with H20
Choose 2-3 slices, place on the slide
Add 3-5 drops of Sudan stain
Cover with cover slip
Allow to stand up for 25 minutes
Remove cover slip, wash with 50% EHOH
Blot dry using f.p. strips
Add 2 drops glycerin, cover with c.s.
Observe under LPO/HPO
Question 1. How much are the cells magnified under the LPO and HPO? LPO=40x ; HPO=100x
Question 2. Draw some cells with oil globules. Label and color the parts of the cells.
Lipids are biomolecules which are abundant in seeds and fruits. They form a component of the cell membrane. Lipids are important to the body as they provide energy, keep the body warm and protect organs from injury.
Question 3. Draw some cells. Label the parts. Color the lipid.
Question 4. Describe the lipids that you observed in peanut and coconut endosperm.
The lipids on the peanut are spherical-like shape while those in the coconut are elongated.

Question 5. What roles do lipids play in the human body?
Lipids constitute the principal structural components of living cells and also provide energy to the human body.
Results and Discussion
The shape of the peanut cell is spherical. We can also see the oil globules on both specimens. The shape of the coconut cell is elongated and we can clearly see the lipids on both specimens. Lipids are important molecules in our body.
Generalization
Lipids are important in keeping our body warm and in providing us energy to be able to do things regularly. it also protects our organs from injury.
Full transcript