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Electrospun PAA with chlorhexidine
Transcript of Electrospun PAA with chlorhexidine
Drug release study Applications Electrospun Poly(acrylic acid) (PAA) with Chlorhexidine Initial project: Electrospun PAA scaffold with doxycycline hyclate
Modification: Switched to chlorhexidine
Analytical Technique: Scanning Electron Microscopy (SEM)
Goal: To observe surface morphology of the fiber structures with drug aggregates Experiment Preparation of PAA solution 1 3 Electrospinning Thermo-crosslinking and Neutralization 2 Incorporation of Chlorhexidine 4 SEM 5 chemical structure of chlorhexidine* References http://www.medichem.es/pdf/medichemChlorhexidineCatalogue.pdf
Yu, D.G., Zhu, L.M., White, K., Branford-White, C. (2009) Electrospun nanofiber-based drug delivery system, Health, 1, 67-75.
http://sims.ess.ucla.edu/resources/SAMPLEPREP.php *http://chemistry.about.com/od/factsstructures/ig/Chemical-Structures---C/Chlorhexidine.htm Strong base
form stable salts with several acids
common salts: gluconate chlorhexidine solution, chlorhexidine acetate
a wide range of antimicrobial activity *Yu, D.G., Zhu, L.M., White, K., Branford-White, C. (2009) Electrospun nanofiber-based drug delivery system, Health, 1, 67-75. SEM Construction* *http://www.purdue.edu/rem/rs/sem.htm Use a beam of electron, instead of light
Information about the sample's morphology, chemical composition, and crystalline structure and orientation of materials
much higher resolution
more control in the degree of magnification
larger depth of field
Polymer sample preparation for SEM
Applications PAA in ethanol with 4 wt% concentration
Ethylene glycol at the concentration of 16 wt% relative to the polymer
The solution was completely dissolved with a magnetic bar for 24 hours Right before electrospinning process, 1M sulfuric acid was added to the PAA solution at a concentration of 50µL/mL
Flow rate: 0.8mL/h
Volume: 4mL PAA nanofiber scaffold was placed in a vacuum oven at 130°C and 30 in Hg of vacuum for 30 minutes
Then the scaffold was neutralized by placing in a base solution containing 1M NaOH and 1M NaCl for approximately one hour and then rinsing with water to remove residual salts 1 mg/mL solution of chlorhexidine prepared (completely dissolved)
Small sample of PAA scaffold with a diameter of 3mm soaked in the solution for 1 hour The sample was completely dehydrated
Small pieces of the sample were mounted on the standard SEM holder with the help of conductive carbon tapes
Then, the samples were sputter-coated with a thin layer of gold
PAA nanofibers were observed using SEM with an operating voltage of 15kV
Data treatment using free software ImageJ SEM sample holder* *http://sims.ess.ucla.edu/resources/SAMPLEPREP.php Fibers with uniform diameter (no bead structures) with an average diameter of 860 ± 40 nm
No chlorhexidine aggregates or crystals observed on the surface of the fibers
Addition of drugs or other molecules at low enough concentrations will not have an adverse effect on fiber morphology - Thank you - Si Young An