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RBC design

I'm just experimenting with the designs. Apparently once you select the theme colour, you can't really edit the colour of the boxes to other colours. This is just my thoughts on it!
by

Emerald Sia

on 22 January 2014

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Transcript of RBC design

37°C
Both set of tubes were incubated at 37°C and ran on Sysmex at 5 time intervals:
15th minute, 60th minute, 120th minute, 180th minute and 240th minute.
One set of 10 secondary tubes was spiked with 1000μl of glucose.

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Saline
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Our Project:
Presented by
Emerald Sia
Priscilla Foo
Diyan

"Marked Interference in Mean Cell Volume Caused by Hyperglycaemia in Samples Analysed by Sysmex XE-5000"

Effect of Hyperglycaemia in Mean Cell Volume in Red Blood Cells Analysed by
Sysmex XE-5000
Hypotheses
1. Observe if MCV is affected
by high glucose concentrations
using Sysmex XE-5000
2. Show that the changes are time-dependent

- Mean Cell Haemoglobin Concentration (MCHC)
- Haemotocrit (HCT)
- Red Blood Cell Count (RBC)
Red Blood Cells
Indices
- Haemoglobin (Hb)
- Mean Cell Volume (MCV)
- Mean Cell Haemoglobin (MCH)
For each concentration:

60th minute
120th minute
240th minute
180th minute
15th minute
😌
Incubation time
Use different haematology analyzers
Abbott,
CELL-DYN Ruby
COULTER® LH 500
Use higher glucose concentrations
Use glucose concentrations:
60mmol/L, 80mmol/L, 100mmol/L, 120mmol/L, 140mmol/L
Future Directions
Use blood from same individual for each glucose concentration
Obtain blood from research participants instead of extracting EDTA tubes from LIS
Sysmex XE-5000
Protocol

Conclusion
1. MCV is affected
by high glucose concentrations
using Sysmex XE-5000
2. The changes are not time-dependent
Increase sample size

Now:
sample size too small for 3 variables to obtain a more definitive p-value for time.
10/20/30/40/50mmol/L
Saline
Glucose
Acknowledgement
Supervisor
Ms Kwek Cheah Yee
Co-Supervisor
Mr Edmund Lui
Lecturers
Ms Sharon Quek
Dr Simon Tan
Khoo Teck Puat Hospital
Frederick
Case Studies
Patient H admitted with blood glucose
of
64mmol/L
and MCV
91 fL
.
During treatment, values fell to
11.5mmol/L
and
85.5fL
respectively.

Patient S. admitted into hospital with glucose level of
97mmol/L
and MCV
111fL
.
MCV fell overnight to
90fL
which corresponds with decrease in blood glucose after treatment with insulin and saline.
Fick's Law of Diffusion
Glucose concentration 50mmol/L has the steepest concentration gradient
1. Active Transport
2. Facilitated Diffusion
3. Altered Diffusion
Effect of Diluent in Hyperglycaemic Erythrocytes
Fair distribution of glucose between RBC and plasma in vivo
Loses the fair distribution of glucose between RBC and plasma in vitro
Transport Mechanism
Discussion
Hans L.P. van Duijnhoven, Marco Treskes (1996), Marked Interference of Hyperglycemia in Measruements of Mean (red) Cell Volume by Technicon H Analyzers, Clinical Chemistry, 42(1), pp. 76
p-value: 0.281
p-value: 0.504
p-value: 0.504
p-value: 0.361
p-value: 0.217
p-value: 0.189
p-value: 0.286
p-value: 0.059
p-value: 0.811
p-value: 0.110
p-value: 0.410
p-value: 0.715
p-value: 0.636
p-value: 0.161
p-value: 0.386
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p-value = 0.001
p-value = 0.002
p-value = 0.202
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p-value = 0.005
p-value = 0.024
p-value = 0.018
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p-value = 0.016
p-value = 0.004
p-value = 0.025
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p-value = 0.003
p-value = 0.010
p-value = 0.305
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p-value = 0.014
p-value = 0.009
Lengthen total incubation time
Shorten individual time intervals
20mmol/L
50mmol/L
40mmol/L
30mmol/L
10mmol/L
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Full transcript