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The Effects of Temperature on Catechol Oxidase stability

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by

jay kesse

on 19 October 2013

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Transcript of The Effects of Temperature on Catechol Oxidase stability

Background info
:
Enzymes
are biological catalysts, that speed up chemical reactions by lowering the amount of
activation energy
(Ea) that is needed for the reaction to proceed. In this experiment we used the enzyme Catechol Oxidase, which is extracted from potatoes.

When this enzyme catalyzes in a reaction where it is exposed to oxygen, it becomes responsible for the brown tint fruits get when they are cut.
the optimal temperature for potatoes is around 40 degrees Celsius.

Reasons for doing the experiment
:
Enzymes do not function as well in conditions which involve temperatures at both extremities, so in this experiment we wanted to tested how changes in temperature influenced the reaction rate of the cathechol oxidase catalyzed reactions.
we also wanted to see whether based on the results, we could estimate the natural habitat range for potatoes (be it in high or low temperatures)

How does temperature affect the activity of catechol oxidase?
Hypothesis
:
Ho
The temperature will not affect the activity of the catechol oxidase.
Ha
The enzyme will function best at temperatures closest to the optimal temperature, so temperatures at both extremities of 40 degrees Celsius will have lower to no reaction rates.










The Effects of Temperature on Catechol Oxidase Stability: Judith Kesse-Enah
Materials
4 cuvettes (blanking cuvette included)
2 5ml pipettes
catechol
catechol oxidase
dropper
3 water baths (37*c, 51*c, 21*c)
spectrophotometer
timer


Procedure
Number 4 cuvetts from 1-4
In the first 3 cuvetts put 1ml of 37*c, 51*c, and 21*c water from the water bath using a pipette (one temp water per cuvette).
In the 4th cuvette put 1ml water at room temperature.
with another pipette, take 2ml of catechol oxidase and place them in the first 3 cuvettes
Add 5 drops of catechol in all 4 cuvettes using the dropper.
Start the process by blanking the spectrophotometer at 490 wavelength with the 4th cuvette.
starting at zero minutes place the first 3 cuvettes in the spectrophotometer one at a time and record your data with a 2 minute interval.
After every 2 minutes blank the ispectrophotometer again and record your data for 28 minutes
after recording your data for 28 minutes, repeat this process and conduct a 2nd trial to increase accuracy.
record your data for the second trial and average out your results for each temperature.
graph the results and see if it supports your thesis
Results
Conclusion
INTRODUCTION
Controlled variable

wavelength at 490 for all
same size cuvettes
5 drops of catechol in all 4 cuvettes (including blank)
Same amount of cathechol oxidase was used.
Protocol
Each of the 3 cuvettes contain water at different temperatures.

Because 37*c is closest to 40*c which is the potato's optimal temperature, it had the highest reaction rate compared to the other temperature
Warningggggggg
during this experiment keep
gloves
and
safety glasses
on.
while taking catechol,
leave hood at halfway mark
dispose all used items to
blue bag
under hood
do not dispose all used liquids into
original container
Pictures
Googleimages.com
Full transcript