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Cheek cells & Onion Cells Lab

Ms. Mirshafie

on 13 April 2016

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Transcript of Cheek cells & Onion Cells Lab

Microscope Lab 2
Cheek Cells & Onion Cells!
To observe onion cells under the microscope:
Animal cells: Human cheek cell
What is a cell?
A cell is the basic unit of life; all living things are composed of cells
There are two types of cells:
Eukaryotic vs. Prokaryotic
Prokaryotic cells were the first types of cells.
They are smaller and less organized than eukarytic cells, with no membrane-bound organelles.
Bacteria and Archea are prokaryotic.
Eukaryotic cells evolved from prokaryotic cells;
They are much larger and have membrane-bound organelles for specific functions.
Plant and animal cells are eukaryotic
TM= 40x
TM= 100x
TM= 400x
To observe the human cheek cells under the microscope:
I used the flat side of a toothpick, reached inside of my mouth and gently scraped the inside of my cheek
I added one to two drops of methylene blue dye to a slide and swirled the flat end of the toothpick into the dye
Using the angle, I placed the cover slip on top
Focused the specimen's image on the low power objective lens and then observed the cells with low and high power magnification
1. Microscope (One for each pair)

2. Iodine solution

3. Microscope slide

4. Cover slip

5. Eye dropper

6. Beaker of water

7. tweezers

8. Small onion piece
To observe onion cells under the microscope:
to peel the translucent inner layer from a
small piece of the onion

Flatten this layer smoothly in a drop of water on a
microscope slide
- create a wet mount slide
Using the angle, put a
cover slip
on top of the onion layer
Add one or two drops of

iodine solution
at the edge of the cover slip
Draw the stain under by touching the
absorbent paper (lens paper)
to the opposite side of the cover slip.
6. Using the
, focus on the onion cells on
low power
lens, then switch to
medium and high power
lens, then observe the cells again.
Watch how this lady prepares a wet mount of the onion cells:
Full transcript