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DNA: Replication!!

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by

Kevin Goodenough

on 6 May 2014

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Transcript of DNA: Replication!!

DNA Replication
DNA Replication consists of 3 basic steps:
DNA Replication:
Proteins

DNA Replication

The process of making a copy of DNA is called DNA Replication
In DNA Replication, the DNA molecule unwinds, the hydrogen bonds break, and the two backbones split.
After that, some new nucleotides are added to each unwound DNA strand until two identical sequences result.
DNA Replication:
Proteins
DNA Polymerase is a protein that catalyzes (helps/speed) the formation of DNA. At the replication fork, these proteins move along each strand and add nucleotides that match each base to form the 2 new double helixes.
Proteins/Enzymes
The suffix "ase," as is Helic-ASE,is used to show that the protein/enzyme does a specific function
Most enzymes are proteins!!

DNA: Replication!!

It's unreal!! How does copy itself?

Warm Up:

What do we remember about DNA Structure?
I will tally points for each half of the room on the board
The team with the most points wins!
Take a minute to think about everything we talked about on Tuesday
RAISE YOUR HAND to respond to get your side of the classroom
If you shout out answers when your hand is not raised, the other team can "steal" your answer for a point.
Step 1: Watch video
Step 2: Respond to the amazing question
(I will give you
the question after
the video)
Why do the video clips of Bigfoot sightings not prove its existence? (Think scientifically!)

Unwinding and Separating DNA strands;

The double helix unwinds and the 2 complimentary strands of DNA separate and forms a “Y” called a replication for fork.


Adding Complimentary bases
• At the replication forks, new nucleotides are added to each side and new base pairs are formed. The original strands serve as a template for 2 new strands. As the nucleotides are added, 2 new double helixes form. This step continues until the whole DNA sequence has been copied.

Formation of 2 Identical DNA Molecules
• 2 identical DNA molecules are produced. Each double stranded DNA helix is made from 1 new strand and 1 old strand of DNA. The nucleotide sequences in both the new and old DNA strands are identical .

DNA Helicase is a protein that unwinds the DNA double helix during DNA replication. These proteins get in between the 2 strands of DNA and break the hydrogen bonds between the nitrogenous base pairs. This separation forms the replication fork.

• DNA Polymerase also has a “proofreading” function to stop errors from occurring during replication. If an error occurs, the DNA polymerase can backtrack.
Exit Question: What would happen if DNA from some of our cells couldn't replicate?
EXAMPLES
Helic
ASE
unzips the DNA molecule
Polymer
ASE
assembles the new DNA double helix
Peroxid
ASE
breaks down peroxide
(Think hydrogen peroxide)
The job of enzymes are to speed up or assist a process. In other words, they
catalyze
reactions.
Full transcript