Transcript: What's on the menu ... What makes us different ? The open framework The managed flexibility Empowered de-central organization Customer & business driven approach Time-to-market Multi-Customer 2009 Decentral Competence Centre Bart Verellen Globally Available Architecture Community 2008 Joana Lomelino (cc) image by nuonsolarteam on Flickr DHL Freight LLP PLATO Recognition as standard for LLP Europe Managed Inhouse 2006 Intro Organisation 2010 Organization Services Architecture Services 2011 Europe Deployment Tommy De Vos Organization Chart Cederik Haverbeke Benelux Deployment Governance End-2-End TMS Live Demo Dieter Lousberg Competence Centre Serge Gregoir Our Customers Key Users Super Users
Transcript: Atomic Troubleshooting Be Careful of sharp objects And Spiders Start What Type of Issue is it? >PS_EAGREE >FILELOADER_INC >Etc. >FM_### >FILE_MOVE_### >FILE_COPY_### >####_COPY >Etc. Move/Copy Move/Copy Start by opening the log in the email of the failure. Look at the section starting with "parameter file" The Two File Paths listed under Parameter File will tell you the from and to of the file move. Pull up both File paths and identify which of the following issues is present. Notes and Screen shots for detailed breakdown of this step Incorrect File Path: What to look for... Click to edit text Thing A Thing B Thing C Multiple Files: Click to edit text Click to edit text No File: Click to edit text Click to edit text File is there: Click to edit text Click to edit text Our Team Error
Transcript: -I had a specific song in mind which was central to my concept; a self-recorded version might prove to devalue this concept -Additionally, finding a group of people capable of playing the song and also willing to record could be a highly difficult task http://www.imagearts.ryerson.ca/ewood/cestlavie/ Solution To create a video that incorporated kinetic typography, video and photography elements, as well as self-recorded audio. Progress http://25.media.tumblr.com/70b66009e6d9f46cfb24df1f93a78d3b/tumblr_misxpq6bSj1qg3wdfo1_1280.png Down-Falls: Original Idea: Proof of Concept I decided to revise my project so as not to diminish the concept. I will now be creating a web-based interactive video in which users will have to click through and view the story of individual characters in order to get the "full picture." This full picture will manifest itself as my completed kinetic typography. Each time a character's profile is viewed, a corresponding section of the typography will be revealed. If no character profiles have been viewed, an instrumental version of the song will play along with different, lyricless version of the video. Sticking with the theme of suicide discussed in my initial proposal, this serves comment on how there are many underlying causes of suicide that are often overlooked until it is too late.
Transcript: Survey conducted online and on paper with IBA students Focus group conducted on campus Survey conducted online and on paper with IBA students 23% percent were willing to buy it for 450 Proof of Concept Methodology Notebooks: 130 per unit; 140 per unit Shirts: 110 per unit 120 per unit, 130 per unit; screen printing : 60 per unit Fair fan graphics and printers : 130 rupees 03212449592 basement Kohinoor square, Pakistan chowk Shirts: Grami garments 110 rupees (03332105303) Office no 47, Rex centre 1st floor, Zaibunissa street Khans company 60 rupees for screen printing. Showroom no. 80 1st floor Rex centre, Zaibunissa street, Saddar Sell the shirts at 400 and notebooks at 200 per unit Initially manufacture 100 notebooks and 100 shirts Costs: Approximately 14000 for notebooks and 20000 for shirts Financial decisions 14% were willing to buy it for 300 The customers were generally curious about the product image Data: 80% were willing to purchase it Conclusions Reaching our customers Interest in the product Quotes from suppliers 44% percent were willing to puchase it for 500 or more Notebooks will incur a cost of 130. 70 rupees per notebook profit T-shirts will incur a cost of 180 profit will be 220 rupees per shirt Gross margin Calculations LoudWolf. 19% percent were willing to buy it for 350 Facebook page Campus stall Simple bags with LoudWolf. logo Discounts when multiple shirts bought It would be better to make shirts in lesser amounts first even if it affects our profit margin Our calculations about market size was remained fairly unchanged Desired Attributes
Transcript: Web Shop Locating a Price Look Up code Head Office Mobile Pos 2 Pos 1 Live View HHT
Transcript: KEY RESOURCES LINE OF SIGHT CUSTOMER SEGMENTS COST STRUCTURE CUSTOMER RELATIONS AFTER the STITCH Questions? CHANNELS BEFORE the STITCH REVENUE STREAMS KEY PARTNERSHIPS CASH FLOW
Transcript: Issues Motor part We calculated the water debit We determined the value for 10% of water left in the tank When the sensor calculate a value inferior to 10%, a red light switches on Embedded System Code d'Anthouard Charles Floret Anthony Ferraz Nicolas Code Material Motor replaced by a LED Raspberry Transmission from computer Website Raspberry part set up a network server Transmit the value from the Arduino to the website Code If the humidity sensor reach a certain value, the motor will start up and the water will pour in the dirt. Humidity sensor part Solution Context Water level part Your plants are dying because of rought. It can be: negligence holidays not a green-thumbed expert You drowned your plant! Proof Of Concept Calculate the humidity values Values transmission to the computer with an Arduino code:
Transcript: Questions? Comments? Concerns Discuss Neural Cell Line Purpose Fully functional neurons derived from iPSC Collaboration with Dr. Navara Pros Finalize Procedures Cancer cells are depolarized, therefore expressing a more positive membrane potential than neurons G-particle's negative charge will be attracted to the more positive cancer cells and, repulsed by more negative neurons Proof of Concept Cure some cancer Change the world Just another day in G-Lab -90 mV Easy to culture Experience Purchase from Perkin Elmer Parental line: ATCC® HTB-14™ Red-shifted luciferase gene (Red- FLuc) brighter, red shifted signal longer wavelengths brighter than other luciferases Eagle's Minimum Essential Medium + 10% FBS Run Experiment Collaboration - we can't culture yet Risky depending on another lab No experience working with this cell line Derived from stem cells - validity Neurons are delicate Imaging Not human Validity: Neuron-like Cancerous Co-Culture these bad boys together Weigh pro's/cons make an executive decision Buy pc12's or ask for Neurons Purchase from Perkin Elmer Parental line: ATCC® HTB-14™ Red-shifted luciferase gene (Red- FLuc) brighter, red shifted signal longer wavelengths brighter than other luciferases Eagle's Minimum Essential Medium + 10% FBS Both are "acceptable" cell lines BUT neither is a primary line Co-culture Red Spectrum Excited with luciferin Emits at 600 PC12 iHPSC Neurons To establish a novel technique using a delivery agent that can alter the desired target’s biological function with various layers of specificity. Rat, not human Neuron like not actual neurons Membrane potential -80 mV not -90 mV Diseased with cancer Differentiated Neurons GBM Cell Lines Human primary glioblastoma line Stage IV ATCC® HTB-14™ Effects in nude mice when ioculated subcutaneously (10^7) Eagle's Minimum Essential Medium + 10% FBS - Determine all areas we would like to investigate - Compile a complete list of claims - Possibility of concurrently run publication experiments - Example: Modeling the BBB in vitro? Method Experience working with this line Membrane potential at -80 mV, similar to neurons at -90 mV Less expensive to buy No training required Easy to culture Typically, highly proliferating cancerous and non-cancerous cells are known to have depolarized membrane potentials G-Particle exploits membrane charge for increased specificity in targeting cancer cells Future projects Human -90 mV MP Neurons Ensure that GBM doesnt eat up all the media and kill the neurons before we can run experiments Cons Hypothesis "Ideal Line" The Debate Red Spectrum Excited with luciferin Emits at 600 G-Particle will selectively target U-87 (GBM) cells grown in co-culture with healthy neurons, demonstrating a safe and effective treatment for cancer Human - stem cell induced Neurons Locally available Cheap: we would proably only pay for dye -90 mV membrane potential Non diseased culture Can also culture glial - a bit longer Gives us experience working with these cells for future projects U 87 MG Cy 3 ex: 550 nm em: 570 nm Buy U-87 MG Pros Game Plan (so far) Imaging Collaboration Risks No experience Validity: induced SC Delicate U 87 MG Bioware® Brite Cell Line U87 MG-Red-FLuc Brain Cell Lines GBM Cell Lines ATCC® CRL-1721™ Rat adrenal gland Pheochromocytoma Imaging Human primary glioblastoma line Stage IV ATCC® HTB-14™ Effects in nude mice when ioculated subcutaneously (10^7) Eagle's Minimum Essential Medium + 10% FBS Final Decision?? Neurons/Pc-12: Cy 3 exites at: 550 emits at 570 U-87 MG: Luciferin Emits at: 600 Nanoparticle: exited: 980 emits: 350 & 680 Cons Bioware® Brite Cell Line U87 MG-Red-FLuc GBM cell line: U 87 Neurons: two options to discuss 1. Differentiated from iPSC 2. PC12 Human Neurons Primary line Easy to culture Experience G-particle Gain experience working with line Grow in EMEM Grow in Neural Media Observe growth rates Estimate Co-Culture cell concentration and account for enriched media Membrane Potential (Vm) PC12
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