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lab meeting presentation

Transcript: Sandeep Kumar oral biology university of Leeds Supervisors: Dr E L Mostafa Dr Jinous Tahmassebi Dr Farideh Javid Identification of the abilities of Cannabinoids to address deterrent inflammation during periodontitis and their role during periodontal tissue regeneration. Title Sandeep Kumar PhD Second Year Supervisors: Dr EL Mostafa Raif Dr Jinous Tahmassebi Dr Farideh Javid introduction commonly found disease, incidence depends on age, sex and socioeconomic conditions.Indirect costs due to oral conditions worldwide amounted to US$144 billion yearly. Journal of Dental Research 2017, Vol. 96(4) 380–387 Periodontium and Periodontitis Gradual inflammation of periodontal tissues i.e. periodontitis leads to loss of bone, eventually loss of function Implications of periodontal diseases J Can Dent Assoc 2000; 66:594-7 environmental perturbations in the periodontal tissues 1. trauma 2. idiopathic growth of a keystone pathogen 3. host is genetically susceptible pathogens elicit inflammation changes the nutrient foundation of the ecological niche (i.e. periodontal pocket). The altered nutrient foundation promotes the proportional expansion of pathobionts relative to symbionts, promoting inflammation that ultimately leads to connective tissue and bone destruction. ETIOLOGY:PERIODONTITIS periodontitis oral hygiene maintenance local drug delivery -- antibiotics systemic antibiotics surgical approaches anti-inflammatory modulation NEWER APPROACHES TO ADDRESS PERIODONTITIS Li Dehao et al. 2017 Cannabidiol(CBD) administration reduces sublesional cancellous bone loss in rats with severe spinal cord injury Fabisiak A et al. 2017 Cannabinoids as gastrointestinal anti-inflammatory drugs. Li et al. 2013 Anti-Inflammatory Role of Cannabidiol (CBD) and O-1602 in Cerulein-Induced Acute Pancreatitis in Mice Ribeiro A et al. 2012 Cannabidiol(CBD), a non-psychotropic plant-derived cannabinoid, decreases inflammation in a murine model of acute lung injury AIM canabinoids: 1. acts as anti-inflammatory agents 2. acts in preventing bone resorption 3. got receptors in human body 4. acts on endocannabinoid system Periodontitis : 1. Is a inflammatory disease 2. causes bone resorption 3. canabinoid receptors are found in periodontal ligament Review To evaluate the ability of cannabinoids to address the chronic periodontal diseases. objectives 1. To investigate the anti-inflammatory effects of cannabinoids. 2. To assess the effect of cannabinoids on osteogenesis. 3. To assess the effect of canabinoids on osteoclastogenesis. Recently it is also found that the endocannabinoid system does not just respond to the endocannabinoids produced in the body, but also respond to external cannabinoids like the phytocannabinoid cannabidiol, or CBD. Marcelo H. Napimoga et al. 2014- used CBD and showed anti-inflammatory effects -------- experimental periodontitis in rats CANNABINOIDS IN PERIODONTITIS Abidi A A et al. 2018 targeted the endocannabinoid system, showed it could offer a new therapeutic approach for treating chronic inflammation in periodontal disease. Niyogi et al 2019 showed canabinoids have antibacterial property against periodontal pathogens IN-VITRO model LPS/Flagellin ------ TIG-K cells/ periodontal cells --- inflammatory response, at m-RNA level and protein level Interleukin-8 (IL-8) plays vital role in pathogenesis of periodontal diseases. inflammatory pathway involved confirmation of pathway using knockdown and gene silencing Results Key Results TIG- K (GINGIVAL KERATINOCYTES): OBSERSEVED EXPRESSION cytotoxicity (LPS, Flagellin and Canabinoids ) il-8 transcription at 3 hours il-8 translation at 3 hours il-8 translation at 24 hours Analysis Results replicated using Periodontal cells from various donors and also with CBG( canabegerol) AEA (Anandamide)/2AG (2-Arachidonoylglycerol) CBD (Canabidiol)/ CBG ( Canabegerol) Subtopic 1 cell viability TIG-K viability with test drug CBD CBG, AM-251- slective antagonist CB1 O-1602 - Non- Selective Antagonist AM-630- Selective antagonist CB2 Subtopic 2 Transcription analysis 1 uM Subtopic 3 Translation AT 3 HOURS AT 24 HOURS 1 uM 1 uM Conclusion Conclusion Pre-treatment with CBD markedly attenuates the expression of IL-8 stimulated TIG-K/periodontal cells with obnoxious stimulus flagellin. This non-psychotropic cannabis product (CBD) may therefore play a vital role in addressing chronic periodontal problems. What’s next What’s next To identify other inflammatory cytokines like IL-6, IL-1b and TNF-α at mRNA and protein levels Genes regulating the mineralization i.e. RANK/RANKL and OPG will be studied. Identify their role in bone mineralization and demineralisation during Periodontitis. Thank you Thank you REFERENCES REFERENCES

Meeting Template

Transcript: The Importance of Your Brand Luke Batty Your Brand Website importance Website 75% of people judge companies based on their website design 61% of mobile searchers are more likely to contact a company that has a mobile friendly site. 84% of user would abandon a purchase if data was sent over an insecure connection. Conclusion conclusion Having an outdated website can make you lose customers, miss out on money, and will significantly damage your brand. Social Media Social Media There are 3.5 billion active users on social media. those users spend an average of 3 hours on social media per day. that means there are over ten billion hours spent on social media per day conclusion Conclusion Even a small portion of that attention can turn any company into an industry leader. Most small companies fail to create a brand identity. Brand Identity Brand Identity Color improves brand recognition by up to 80% Presenting a brand consistently across all platforms can increase revenue by up to 23%. Conclusion Conclusion having a strong brand across all platform will increase customer loyalty and increase new business Reputation 73% of consumers love a brand because of helpful customer service. Reputation A single business review can lift its conversions by 10%. 86% of consumers would consider writing a review for a business. conclusion Conclusion having positive reputation is not only important it is essential to the survival and growth of a small business and reviews are the lifeblood of that reputation Online Directories Online Directories There are over a dozen different FREE directory services that your customers are looking at EVERY DAY. Who We Are

Board Meeting - Presentation Template

Transcript: Template by Prezibase.com Presentation Headline About There's no wrong time to make the right decision. Dalton McGuinty " A presentation template with a board meeting theme for introducing your business. Showcase Your Ideas Agenda Talk to people about your presentation Meeting Agenda Welcome Vote on New Secretary Eat Donuts Add More Details here.... Time Present important dates and events here Business Timeline Present important dates and events 1999 Present important dates and events 2002 Present important dates and events 2006 Zoom in for the fine print 2020 Present important dates and events 2000 Present important dates and events 2004 Present important dates and events 2011 Sales Business is slumping? Fire the guy on the next slide! How are the Sales? Item 1 Item 2 Item 3 Item 4 Item 5 Item 6 CEO A man must be big enough to admit his mistakes, smart enough to profit from them, and strong enough to correct them. John C. Maxwell " Gallery Show what's going on in your company Make a Gallery Image #1 Image #2 Image #3 Image #4 Image #5 Image #6 Show more details of a single project here Single Item Funky Title Here "This is example dummy text. Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed dodes eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in repre henderitas in voluptate." Map Display your new business ventures Conquer the World? - Add details here - Add details here - Add details here - Add details here Team Who are in the same boat? Meet The Team Gerardo M. Oliver CEO Analyst Sales Developer Karla J. Walsh Bradley Sherman Sue E. Marquez Show more details about one person Single Person View Senior Data Analyst Karla J. Walsh This is an example dummy placeholder text area. Lorem ipsum dolor sit apisa kotiram ametis, cons iteecas tetureu adipiscing elit sed quia, Contact How can People Get in Touch with You After a Great Presentation? Contact Details email@mail.com website.com fb.com/user.com lnked.in/user +1-202-555-0184 Change colors, rearrange topics, add your own content Customize this template: https://prezibase.com Get this Prezi Template from:

Lab Meeting Presentation #1

Transcript: Detecting Yeast tRNA Nucleotidyltransferase Protein:Protein Interactions Using the Yeast Two-Hybrid System Positive interaction= transcription will occur ARP6 Transformation into E.coli ARP6 Function: component of glycolysis and gluconeogenesis --Also assists in importing tRNA into the mitochondria Size: 1314bp Potential: Identified physical interaction with CCA1 Positive interactions are important for localization of tRNA-NT Helps us see effects of selected proteins on tRNA-NT 1500bp Using the yeast two-hybrid system to detect protein:protein interactions between bait (CCA1) and selected proteins. Confirming positive interactions found by high-throughput methods Why is this important? 500bp Function: Nuclear pore protein involved in --nuclear export of pre-tRNA --re-export of mature tRNAs Size: 3303bp Potential: Identified genetic interaction 1 2 3 P N LRP1 Function: Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, transport, etc. Size: 555bp Potential: Identified genetic interaction Potential Candidates Correct Orientation 1000bp Colony 2- ARP6 inserted in correct orientation Obtained two fragments after digesting with EcoRV: --2,250bp and 4,767bp Ligation of ARP6 ENO2 ARP6 Focus ARP6 Wrong Orientation LOS1 Expected product size between 1000bp-1500bp since ARP6 is 1317bp in size. Overview 1500bp 5000bp Fragments of 2,250bp and 4,767bp Fragments of 1,850bp and 5,167bp What's Next Yeast transformation this week E. coli transformation of MRT4 and GIS2 in the near future Function: Actin-related protein that binds nucleosomes Size: 1317bp Potential: Identified physical interaction with CCA1 ARP6 PCR Product

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