Loading presentation...

Present Remotely

Send the link below via email or IM


Present to your audience

Start remote presentation

  • Invited audience members will follow you as you navigate and present
  • People invited to a presentation do not need a Prezi account
  • This link expires 10 minutes after you close the presentation
  • A maximum of 30 users can follow your presentation
  • Learn more about this feature in our knowledge base article

Do you really want to delete this prezi?

Neither you, nor the coeditors you shared it with will be able to recover it again.


C-Peptide Initial ELISA 3-14-2013

Testing the C-peptide ELISA for dilution and standard

Diana Xu

on 18 March 2013

Comments (0)

Please log in to add your comment.

Report abuse

Transcript of C-Peptide Initial ELISA 3-14-2013

Testing Dilutions and Standards C-Peptide ELISA Choosing Serum Samples Standard Curve Construction Next Steps... Picked samples at the predicted extremes of c-peptide concentration.
-IL-6 concentrations
-leptin concentrations
-Polyp number
Final list of samples
-low extreme: 14, 26, 81
-high extreme:23, 134, 12 C-Peptide ELISA Dilutions
- undiluted
- 1:2
- 1:20
- 1:100
Many concentrations fell below standard curve
- We can run samples undiluted
Samples 14, 26, 81 had lower concentrations
Samples 134, 12, 23 had higher concentrations Standard concentrations were not as they should be
-Leads to errors in calculating c-peptide concentration in samples 1) Run standards and some samples again:
Same method as Thursday w/ standards from the different bottles.
Serially dilute the 10 ng/ml standard to make our own standards

2) Construct new standard curves.
Full transcript