Loading presentation...

Present Remotely

Send the link below via email or IM

Copy

Present to your audience

Start remote presentation

  • Invited audience members will follow you as you navigate and present
  • People invited to a presentation do not need a Prezi account
  • This link expires 10 minutes after you close the presentation
  • A maximum of 30 users can follow your presentation
  • Learn more about this feature in our knowledge base article

Do you really want to delete this prezi?

Neither you, nor the coeditors you shared it with will be able to recover it again.

DeleteCancel

Pharmaceutical Chemistry III

No description
by

mohamed kandeel

on 18 February 2018

Comments (0)

Please log in to add your comment.

Report abuse

Transcript of Pharmaceutical Chemistry III

Logo
Metformin HCl
Pharmaceutical Chemistry III
1st Lab

Drug Monograph
Pharmacopia
Chemical structure
Chemical name
Molecular formula
Powder or dosage forms
Identification
Spectral data
IR spectroscopy
NMR [HNMR or C13NMR]
MS
Identification chemical tests
Purity
Purity tests
Limit tests
Assay [quantitative estimation]
Titrimetry
Acid base
Complexometry
Redox
Argentometry
Spectrophotometry
Calculations
Titrimetry
Target of experiment:
calculate drug amount.
Data of experiment:
mls of standard used
To convert: equivalence factor [F]
1 ml standarad = ?? gm of sample analyzed
Note that:
Standard used in calculation of F: titrant if direct titration and known excess if back titration.
Molar and normal solution.
Standardization factor [f]
Dilution factor
• If make BACK titration: convert mls to (known xss – mls)
• If dilution : divide by initial volume.]
• In R %: F & theoretical wt must be in the same unit [gm or mg]
• Concentration per tablet or per ampoule.
Spectroscopy
Calibration Curve:
Oral Anti-diabetic
Powder
Purity
Limit test of Heavy Metals [H.M]
Primary Tube
12 ml Metformin Sample For Purity
2 ml Acetate Buffer
1.2 ml Thioacetamide
Auxiliary Tube
2 ml Std Pb [10 ppm]
+ 2 ml Metformin Sample For Purity
+ 8 ml Dist. Water
2 ml Acetate Buffer
1.2 ml Thioacetamide
Compare Yellow Color
Results
Primary > Auxillary [Above the limit]
Primary ≤ Auxillary [Within the limit]
Assay
Principle:
Metformin hydrochloride is assayed quantitatively by
non-aqueous titration
.

Add
mercuric acetate
[
librate free base
] and titrate with Perchloric acid.

E.P. is determined
potentiometrically
Tablets Assay
Principle:
Metformin hydrochloride tablets can be assayed quantitatively by
spectrophotometry
since the drug has an absorbance in the UV region (maximum at 232 nm).
Powder
Purity
Assay
Tablet Assay
Procedure:
10 ml
Glibenclamide sample for assay
[containing
0.55 gm
] + 0.5 ml ph ph, titrate with 0.1 M NaOH till 1st pink end point.
1 ml 0.1 M NaOH is equivalent to
49.40 mg
of Glibenclamide
Calculate
C % and R %


Procedure
:
Powder one glibenclamide tablet [
5 mg
], shake with 50 ml [equal volumte of ethanol + 0.1 M HCl] for 10 min.
Complete volume to 100 ml with the same mixture in a measuring flask 100 ml.
Filter and measure absorbance at 300 nm taking
63 as A (1%, 1 cm)
Calculate
CT and R%

Principle:
Glibenclamide could be assayed by
measuring the absorbance in the UV region
(maximum at 300 nm) after extraction of tablets with methanolic HCl.
Identification Tests
[1]
Powdered tablet + water; filter
Filterate + NaOH + 1-naphthol + Na hypochlorite : orang red color
[2]
• Powdered tablet + water; filter
• Filterate + HNO3 + AgNO3: white ppt
Mohamed Kandeel; M.Sc.
Full transcript