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Lab fermentasi

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Hikaru Kou

on 9 October 2012

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Transcript of Lab fermentasi

Kelompok 10:
Madriena(2010-080-007)
Manda M(2010-080-023)
Theresia(2010-080-038)
Stevanus A(2010-080-056)
Teodora A(2010-080-076) OPTIMASI PRODUKSI ETANOL
DARI HIDROLISAT JERAMI PADI MENGGUNAKAN INOKULUM YANG SESUAI Indonesia NegaraAgraris PADI Limbah Beras jerami sekam batang LIMBAH TERBANYAK!!! TUJUAN Menemukan inokulum yang dapat memecah gula pentosa hasil hidrolisis jerami padi yang dapat hidup co-culture dengan S.cerevisiae sehingga diperoleh yield etanol yang optimum Luaran Jurnal science berkala Indonesia LATAR BELAKANG Cair, tidak berwarna tidak beracun--> aman bagi lingkungan bahan bakar alternatif --> Renewable Produksi dari biomassa dan tanaman Bioetanol TINJAUAN PUSTAKA KADAR PATI TINGGI jagung gandum tebu FERMENTASI BAHAN LIGNOSELULOSA kayu selulase Tahapan Produksi Bioetanol Hemiselulosa dan selulosa monomer gula etanol fermentasi Saccharomyces cerevisiae Khamir anaerob
fakultatif budding, filamen/hifa sumber energi --> fermentasi gula memfermentasikan:
glukosa
mannosa
fruktosa gula pentosa Proses metabolisme S. cerevisiae mengubah gula heksosa etanol:
Gula difusi melewati membran plasma sel
Jalur glikolisis Embden-Meyerhof
Gula teroksidasi jadi piruvat, menghasilkan dua ATP
Fermentasi: piruvat NADH dan alkohol Clostridium termofilik anaerob selulosom --> degradasi selulosa, hemiselulosa Fermentasi gula hasil degradasi ----campuran alkohol proses --> 12 hari Trichoderma reesei Pembusuk kayu selulase ---> degradasi lignoselulosa Fermentasi --> gula pentosa dan heksosa Kemampuan degradasi hidrolisat
Enzim selulolitik
pemanasan Produksi tinggi di seluruh duniasumber pangan
Hasil sampingan: biji kering, bagas, jerami
belum dimanfaatkan dengan baik
Jerami
tidak berbiji
Jumlah 50% dari total berat tanaman
Lignoselulosa tinggi sumber energi
Jerami dapat digunakan sebagai substrat produksi materials references Fermentasi GULA dari Jerami gula sakarifikasi substrat inokulum S. cerevisiae, Clostridium spp. nutrisi tambahan amonium sulfat (0.3% b/v)
natrium dihidrogen fosfat (0.15% b/v)
ekstrak khamir (0.5% b/v). fermentasi kondisi diam suhu 30 C pengukuran kadar alkohol kolorimetri references [EREC] European Renewable Energy Council. 2012. Bioethanol production and use [terhubung berkala]. http://www.erec.org/fileadmin/erec_docs/Projcet_Documents/ RESTMAC/Brochure5_Bioethanol_low_res.pdf [1 Okt 2012].
[ESRU] University of Strathclyde Engineering. 2012. What is bioethanol? [terhubung berkala]. http://www.esru.strath.ac.uk/EandE/Web_sites/02-03/biofuels/what_
bioethanol.htm#what_bioethanol [29 Sep 2012].
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