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Evaluation of Chemical Disinfectants against P.aeruginosa isolates (project)

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Ezdehar Gh

on 5 September 2014

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Transcript of Evaluation of Chemical Disinfectants against P.aeruginosa isolates (project)

Evaluation of Chemical Disinfectants
Against Pseudomonas aeruginosa Isolates Definitions Hospital-acquired infection, is also known as a HAI 
or in medical literature as a nosocomial infection,
is an infection whose development is favoured by
a hospital environment.
Such as one acquired by a patient during a hospital
visit or one developing among hospital staff. Such infections include fungal and bacterial infections
and are aggravated by the reduced resistance of individual patients. Disinfectant Pseudomonas aeruginosa P. aeruginosa is a gram-negative, strict
aerobic, long rod bacterium with unipolar
motility. It is considered one of the main causes of nosocomial infections worldwide, its antibiotic resistance is increasing in clinical isolates Hospital-Acquired Infection A liquid chemical that can be applied to
objects to eliminate many or all
pathogenic microorganisms with the
exception of bacterial spores How does Pseudomonas aeruginosa
transmit in a hospital environment Contaminated water in a hospital setting
can transmit P. aeruginosa to patients through
direct contact with the water, either through
ingesting or bathing, inhalation of aerosols,
medical devices rinsed with the
contaminated water
or indirect contact from contaminated
surfaces via health worker’s hands. The estimated number of HAIs in U.S. hospitals, adjusted to
include federal facilities, is approximately The estimated deaths associated with
HAIs in U.S. hospitals were Of all hospital-acquired infections,
P. aeruginosa accounts for about 10.1% Centers for Disease Control and Prevention CDC 98,987 Project Materials Chemical Disinfectants 5% Clorox 5% Dettol Hospital disinfectant (neat) 3% Hydrogen Peroxide 20 different Isolates of Pseudomonas aeruginosa QC organism Culture Media Aim of the Study To evaluate the efficacy of chemical
disinfectants against isolates of
Pseudomonas aeruginosa using the
disc diffusion method Practical Steps: Practical Work Preparation Tips
& Pipette Blank discs Densichek reader Cotton swab Distilled water MHA Dilution of disinfectants Obtain pure fresh
cultures from each isolate Dispense 15µl of
each disinfectant Prepare 0.5 McFarland suspension from each isolate Streak the suspension onto the surface of Mueller Hinton Agar Place sterile blank discs Incubate aerobically
at 37ºC for 16-18 hrs Interpretation of results
(Zone of Inhibition in 'mm') Results Hydrogen peroxide
(33.4 mm) Clorox
(16.4 mm) Hospital disinfectant
(7.6 mm) Dettol (6.8 mm) Disc size = 6 mm Mean zone of inhibition Antibiotic Susceptibility Testing VITEK 2 machine Conclusion Hydrogen-peroxide and hypochlorite-based compounds have the highest antimicrobial activity against P. aeruginosa The VITEK 2 antibiograms of the 20 isolates showed 70% sensitivity to both Ciprofloxacin and Gentamicin while Piperacillin/Tazobactam showed the least sensitivity with only 5% Statistical Data from the Infection Control Screening department at KAUH
2011-2012 Take Home Message Regular Hand washing Keeping hand wash in a clean container Decontamination of medical equipment Never leave open containers of disinfectants Project supervisor: Supervisor of the Clinical
Microbiology Lab at KAUH: Dr. Khalil Alkuwaity Dr. Hani Shukri Thank
You IT & Statistics Department at KAUH: Mr. Abdullah Ba-taweel
Mrs. Areej Abu-jabal MOM & DAD Special Thanks To Presented By: Ezdehar Ghazal 1.7 million Contents: -Introduction
-Aim of the study
-Practical work
-Acknowledgments June, 2013 5
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