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Very Simple NGS Overview

This is a very high-level overview of NGS technologies and applications.

Sean Davis

on 6 November 2012

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Transcript of Very Simple NGS Overview

High Throughput Sequencing Tools for Viewing the
Cancer Genome
in High Resolution Gene Expression Chromatin Structure and Function Sequence Variation Copy Number Structural Variation Epigenomic Modifications Cancer Our First Experiment Overview of BAC in the Genome Sequencing a BAC Sequence Coverage Repeats are not created equal Views of the Cancer Genome ChIP-seq and DNAse-seq Exome & Genome Sequencing RNA-seq Structural Variation DNAse HS sites near transcription start sites are associated with actively transcribed genes. DNAse HS Sites and Gene Expression Long oligos synthesized on arrays (DNA)
RNA baits synthesized from DNA oligo template
RNA baits hybridized to DNA sequencing library
Targets captured using beads and biotin-labeled baits
RNA bait degraded, leaving sequencing library enriched for target regions Good SNP Suspect Variant Loss of Heterozygosity Somatic (tumor only) Variant
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