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Chemistry - Candy Chromatography

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Kayla Adair

on 19 April 2013

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Transcript of Chemistry - Candy Chromatography

Paper chromatography is an analytical method technique for separating and identifying mixtures that are or can be colored, especially pigments. Candy Chromatography Separation of plant pigments is the most common use of paper chromatography in biology. Chromatography paper can also be used in crime scene investigations, DNA and RNA sequencing, among other things. Chemists use chromatography paper in labs to track the progress of a reaction. References http://jh399.k12.sd.us/Lab%20Activity/Chemistry/ch22chromatography.pdf
Purpose By doing this lab, it will prove that chromatography allows one to separate different organic compounds from a mixture using variations in solubility, size of the molecules and retention by the paper being used to separate the compounds. Materials •Four different colored M&Ms®•Rubbing alcohol•Two coffee filters•Two plastic cups•Pencil•Ruler•Tape•Foil•Table salt•Water•Toothpicks•Measuring cups•Clean pitcher Procedures First, I cut two coffee filters into a rectangle measuring three by nine centimeters.

Second, I spaced four drops of water an equal distance from each other on a piece of foil. I then positioned one M&M of each color on the drops. I gave the color about a minute to come off into the water, then I threw away the M&M's

Third, I took a pencil and drew a line one cm from the edge of one end of one strip of paper.

Fourth, I made four pencil dots along the line, about 0.5 cm apart. Underneath each dot, I labeled the color of the candy I was testing on the spot.

Fifth, I made a prediction of the components I expected to see from each candy color.

Sixth, I dipped a toothpick into one of the samples of colored water and dabbed the color onto the pencil dot for that color. I then allowed the filter paper to dry, and then I went back and added more color to each dot, repeating this step a total of three times for each color.

Seventh, I prepared the salt solution by mixing 1/8 teaspoon of salt and three cups of water in a clean pitcher. I stirred the solution until it is dissolved.

Eighth, I poured the salt solution into a clean plastic cup so that the liquid level was 1/4 inch.

Ninth, I taped the strip to a pencil and rested the pencil on top of the jar so that the strip hung into the jar. The goal was to have the end of the strip just touching the surface of the solvent solution, with the colored dots above the surface of the liquid.

Tenth, the capillary action drew the salt solution up the paper. As it passed through the dots, it began to separate the dyes. When the salt water was 1/4 inch from the top edge of the paper, I removed it from the cup and placed it on a clean, flat surface to dry.

Eleventh, I repeated steps two through nine, using the second strip of paper and a clean cup, to test the same four colors using the alcohol instead of the saltwater solution at the bottom of the cup. Data and Observations My original predictions were that the salt water would cause the color to come out of the paper and that the rubbing alcohol would eat through the paper. Discussion and Conclusion In the future, I would have used a clear plastic cup or a glass so I could see the process. Instead, I used a red Solo cup because that was the only plastic cups I had. Other than that, I could not do anything differently.
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