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Bioprocess Insulin

Overview of Insulin process
by

Ally Vitali

on 21 January 2013

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Transcript of Bioprocess Insulin

Biopharmaceutical Process Development Manufacture of the active pharmaceutical ingredient Insulin An Overview of the Biological Functionality and Therapeutic Potential of Human Insulin
Image Source: www.shutterstock.com A multi-functional signalling molecule Approximately 5700 Daltons Molecular Weight Produced in the Pancreas A 51 amino acid heterodimeric peptide hormone Product Overview Works alongside glucagon in order to maintain glucose homeostasis

In addition insulin functions to regulate cell proliferation, differentiation and gene expression
Image Source: www.phoenixpeptide.com A heterotetrameric transmembrane glycoprotein At the organism level this leads to coordinated changes in both fat and carbohydrate metabolism At the cellular level this leads to changes in glucose transporter expression together with altered patterns of proliferative behaviour Insulin binding leads to tyrosine kinase autophosphorylation, adaptor protein recruitment and the activation of various kinase -mediated signalling pathways Activities mediated through Insulin Receptor binding Mode of Action
Image Source: www.nature.com These include the molecules ability to self-associate and its isoelectric point Amino acid substitution and rearrangement are employed in order to alter insulin’s molecular properties Various insulin analogues are currently in use, each of which presents a uniquely useful pharmacokinetic profile Primarily used in the treatment of type-1 and type-1.5 diabetes, together with advanced type-2 diabetes. Therapeutic Potential Commonly used as a media supplement during mammalian cell culture Non-therapeutic applications Cancer, chronic weight loss and Alzheimer’s disease Used to treat a variety of additional indications Alternative Applications Fermentation

Usually performed in a stainless steel
high pressure vessel

Sterilised media inoculated with transformed
E. coli bacteria Primary Recovery Trp-LE'-MET-proinsulin is produced as inclusion bodies Disc-Stack centrifuge removes light phase from the heavy phase containing the target protein
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