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Determination of the Anti-fungal Property of Gynura nepalens

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Jhei Pi

on 24 March 2014

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Transcript of Determination of the Anti-fungal Property of Gynura nepalens

Group II
College of Pharmacy

Determination of the Anti-fungal Property of the Crude Ethanolic Leaves Extract of
Gynura nepalensis

(Asteraceae) on Trychophyton mentagrophytes
Presented by:
Binwag, Klarize Kae D.
Caab, Gliezhel L.
Loto, John Paul G.
Mendoza, Raphael Israel C.
Yumang, Noreena Alva C.

ashitaba plant
Gynura nepalensis DC
fungal infections
2nd leading cause of consultation in dermatological disorder.
Trychophyton mentagrophytes
accdg. to Ziegelmann, Rotta I., et.al is the drug of choice for Dermatophytoses
in warm tropical climate like Philippines there is an increased susceptibility for fungal infections
has an anti-microbial property accdg.
to Vijay et.al

A Thesis Presented to the National University
College of Pharmacy
a medicinal herb which belongs to the Asteraceae family
use as remedy for athletes foot and ringworm
Statement of the Problem
What are the phytochemical constituents present in
Gynura nepalensis
DC leaves?
Is the crude ethanolic extract of
Gynura nepalensis DC
dermally irritating?
Is the antifungal activity of the crude ethanolic extract of
Gynura nepalensis DC
as significant as the reference drug, Terbinafine?
Null Hypothesis: There is no significant difference in the antifungal activity of the extract obtained from Gynura nepalensis DC against Terbinafine.
Significance of the Study

1. development of new drugs from natural resources.
2. avoidance of use from synthetic drugs which can harm the host.
3. determine noble therapeutic uses of this plant.
This study will enhance and improve the knowledge of our fellow Pharmacy students about the antifungal property of
Gynura nepalensis DC
This will serve as a reliable reference to the future researchers for their study.
This research can compel government agencies to give focus and conduct more researches on this plant as source of alternative antifungal products.
Scope and Delimitations
Determine the antifungal property of the crude ethanolic extract of
Gynura nepalensis DC.
The plant was procured from the Bureau of Plant Industry and was authenticated at the National Museum, Botany Department.
The fungi Trychophyton mentagrophytes was procured from Department of Science and Technology(DOST). The research experiments and test was conducted at the Environment and Biotechnology Division-DOST and National University while the 1% Terbinafine Cream was purchased at Mercury Drugstore-Bambang.
Review of Related Literature:

Ashitaba (
Gynura nepalensis DC
) belongs to the Family Asteraceae and is also known as Leaves of Gods, Cholesterol Spinach, Dawn Dewa.

Used as remedy for athletes's foot and ringworm infection (Kala, 2005)

According to Nigam et.al., the ethanolic extract of the Gynura nepalensis DC has antimicrobial activity.

One of the study called "The In-Vitro Screening of Antifungal Activity of Plants in Malaysia" revealed that Gynura procumbens, which belongs to same genus as Gynura nepalensis DC, crude methanolic extract exhibit a slight antifungal activity against Trychophyton mentagrophytes.

and this will be beneficial to the community in promoting the quality of life
Also, another study of the same genus, Gynura segetum, by Seow et.al., showed that is has Anti-Microbial Activity.
Phytochemical Test
Test for Alkaloids

Dermal Irritation Test
100% concentration of the plant extract were tested.

Terbinafine HCl is the positive control.

Trychophyton mentagrophytes is the chosen test organism.

Culture Media
Sabauroud's Dextrose Agar is the media.

Statistical Treatment
ANOVA and T-test.
Data Analysis:

Statistical Analysis
The Gynura nepalensis DC leaves was found to have the following constituents namely sterols, flavonoid, alkaloids, saponins, glycosides and tannins.

The test for antifungal property of the extract with 25%, 50%, 100% concentration was used in determining the pharmacological effect of the extract against the reference drug terbinafine.

The following constituents that are found in the Ashitaba (Gynura nepalensis DC) crude ethanolic leaves extract are alkaloids, flavonoids, glycosides, tannins and saponins.
The crude ethanolic leaves extract is not dermally irritating and the 100% concentration of the crude ethanolic extract has a significant antifungal activity to the 1% Terbinafine HCl cream.

Isolation of the specific antifungal constituents.
Test the crude ethanolic leaves extract to other strains of dermatophytes.
Research on other part of the plant for any pharmacologic activity.
Test the crude ethanolic leaves extract to other microorganism.
Determine other possible therapeutic property of the
Gynura nepalensis DC.

Gynura nepalensis DC
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