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MICROBIAL INTERRELATIONSHIP: BACTERIAL SYNERGISM AND ANTAGON

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Andrea Oconer

on 24 March 2015

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Transcript of MICROBIAL INTERRELATIONSHIP: BACTERIAL SYNERGISM AND ANTAGON

occurs when two organisms grow better together than apart (mutualism) or when the by-products of one organism enhances the survival of another without affecting the contributory organisms (commensalism).
the suppression or interference or inhibition with the normal growth of a microorganism by another microorganism, such as bacteria or fungi.
Microbes may compete with another microbe for nutrients and these competitions often result to inhibition of one of the microbes. This is termed as antagonism which is described as the inhibition of a bacterium by the products of another
Antagonism
refers to the reduction in growth and activities of an organism as a result of the living of another organism.
The combination of two microorganisms in a single culture media may indicate
synergism
if both of them can grow in that media.
Antagonism
can be illustrated by growth of a single species on the agar plates.
Antagonism
Synergism Test
MICROBIAL INTERRELATIONSHIP: BACTERIAL SYNERGISM AND ANTAGONISM
What is Synergism?
Two or more organisms act together to produce a substance that none can produce separately
synergistic relationship
Synergism
RESULTS
Methods and Methodology
Purpose
Antagonism Test
The purpose of this experiment is to give the students a simple idea of how bacterial interaction exhibits antagonism or synergism between two species
This phenomenon is readily demonstrated in the ability of some bacteria acting synergistically, to produce gas by fermenting certain disaccharides
Bromthymol Blue as indicator for Carbohydrate Broths were prepared from stock solution using 8g Bromthymol Blue, 250ml of 95% ethyl alcohol, and250ml Distilled Water.
Lactose and sucrose based broths were prepared using 5g sugar base (lactose and/or sucrose), 10g tryptone, 5g yeast extract, 2ml of the indicator solution and 1000ml of distilled water.
Durham tubes were added to nine lactose broth tubes and nine sucrose broth tubes
Each type of broth was inoculated with each microorganism (i.e. each organism was inoculated to one tube containing sucrose broth and the other tube with lactose broth).
(To check for synergism, combinations of two organisms were inoculated in one tube containing sucrose broth or lactose broth.)
The tubes were incubated for 48 hours at 37°C and observed for gas productions and color change.
Nutrient agar media were liquefied and cooled to about 50 C and inoculated with the initial test organism.
These were then poured into sterile Petri plates and allowed to cool at room temperature before streaking with the second test organism.
Observations were made after the plates were incubated at 37°C for 48 hours.
ST2C =(sucrose broth+P.vulgaris)
LT2A=(Lactose+S.aureus)
LT2B=(Lactose +P.vulgaris)
LT2C = (Lac+S.aur+P.vul)
LT1B=(Lactose +E.coli)
LT2B=Lactose + P.vulgaris)
LT3B=(Lac+E.coli+P.ulgaris)
ST1A=(sucrose+E.coli)
ST3B=(sucrose+E.coli+P.vulgaris)
ST2C=(sucrose +P.vulgaris)
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