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Fish Health Exam

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by

Matt Stinson

on 20 August 2013

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Transcript of Fish Health Exam

First comes the external examination:
1) check fins, gills, eyes, mouth, body
2) Prepare a skin scrape and gill wet mounts for microscopy
Scales and mucus are present, but no parasites...
Each gill filament has many branches called primary and secondary lamella. These gills look healthy!
Primary lamella
Secondary lamella
...but these are a couple of parasites that can be found on the skin surface of fishes.
Renibacterium salmoninarum
from a kidney sample labeled with a probe at 1000x magnification.
Check the vital organs: kidney, gal bladder
heart, liver, pyloric caeca, mesentery fats, spleen, intestine
Next, use your bacteria loop to streak a kidney sample onto a petri dish,
and save a kidney, spleen and gill sample for virology.
Fish Health Examination
by Matt Stinson
Microbiologist at the
Lower Columbia River
Fish Health Center

Lets view the samples at 100x magnification
Next comes the internal examination...
This yellow pigmented bacteria is called
Flavobacterium columnare
This bacteria secrets a substance that turns the media brown.
Aeromonas salmonicida
Some bacteria a difficult to grow, so we use a fluorescent marker that specifically binds to the bacteria we are looking for
Healthy cells are evenly spread out like a cobble stone road.
Uninfected CHSE-214 cells
CHSE-214 cells infected with IHNV
A virus can replicate within a cell and spread to the surrounding cells killing them in an orderly fashion.
Don't forget to sample the head for Myxobolus cerebralis, the parasite that can cause Whirling Disease!
All the results are keep on record for documentation and future reference.
Place the kidney, spleen, and gills samples you saved for virology into a processing bag. This sample will be seeded onto cultured fish cells.
A petri dish with nutrients and agar can grow certain types of bacteria.
Place tissue samples on a glass slide and cover with a slip
Full transcript