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Antibiotic Resistance in Pathogenic Bacteria
Transcript of Antibiotic Resistance in Pathogenic Bacteria
Using Nitrocefin to test for TEM-1 activity in Gram-negative Bacteria
antibiotic with a beta-lactam ring
in the presence of
is NOT present
Antibiotic Resistance In Pathogenic Bacteria
Norfolk General Hospital - Patient Report
Analyze Stool Sample
Global concerns and challenges concerning antibiotic resistance:
higher death rates
higher cost of medical care
harder to control spread of infection
jeopardizes health-care gains to society
is what makes
Identifying Bacteria Strains
Why Size Matters
Each species of bacteria has DNA of a particular size
(measured in base pairs)
As it happens,
DNA has a negative (-) charge
If we put DNA
into a layer of gel, and place the gel into a magnetic field, the DNA will be pulled through the gel towards the positive (+) end of the field
The largest strands of DNA will move the slowest, and the smallest strands of DNA will move the fastest
This joke is
in bad taste...
So, how do we
DNA to measure
Get on over here, boy
Meh, maybe later...
Results of Gel Electrophoresis
After looking at the DNA samples under UV light, we found that the DNA from Jennifer's bacteria was the same size as DNA from a known strand of Salmonella.
These are the R plasmids
Negative control. No results shown.
~5,000 base pairs
The TEM-1 Gene codes for beta-lactamase enzyme
JENNIFER'S BACTERIA RENDERED THE PENICILLIN INEFFECTIVE
TEM-Gene resides on the
Resistant bacteria can "infect" other bacteria through
Positive for beta-lactamase activity
looking for the R-plasmid
Old Dominion University
Biology 115N- Lab
Jennifer's symptoms were unresponsive to penicillin because she has a
Devise (2) experiments to determine the presence of TEM-1 beta-lactamase in Jennifer's sample
NOT a substrate for TEM-1!