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Chemistry and Chromatography

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by

Rachel Medina

on 12 November 2013

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Transcript of Chemistry and Chromatography

Chromatography
Chemistry:
A World that Matters

Study of composition, structure, and properties of matter as well as the changes that occur in matter
What is Chemistry?
Qualitative and quantitative experiments
Matter
Mixture
Pure Substances
Heterogeneous
Homogeneous
Elements
Compounds
Atoms
Variable composition?
No
Yes
Visibly
distinguishable
parts?
Yes
No
Contains various types of
atoms?
No
Yes
Chemical methods

* “Writing with color”

* A very powerful physical method to separate and analyze mixtures

* Qualitative and quantitative analysis
Chromatography
What does it need to separate?
Stationary Phase – Solid
How does it Get Separated?
Different compounds in the mixture travel through the phases at different rates
Types of Chromatography
Paper Chromatography*
Thin Layer Chromatography (TLC)*
Gas Chromatography
Liquid Chromatography
Ion Exchange Chromatography
Affinity Chromatography etc…

*Quick and inexpensive techniques to qualitatively find
how many components are in a mixture
Why it is Important?
How do we identify the compounds in the Mixture?
Rf = Retention Factor
Candy Chromatography
Mobile Phase – Gas or Liquid

Compounds with a

smaller affinity

for the stationary phase travel

faster

with the mobile phase.
Paper Chromatography*
Thin Layer Chromatography (TLC)*

Gas Chromatography
Liquid Chromatography
Ion Exchange Chromatography
Affinity Chromatography etc…

*Quick and inexpensive techniques to qualitatively find
how many components are in a mixture
check your hood!
Operation: Spotting
Take the pen /or toothpick and spot.
MAKE SURE ALL SPOTS ARE LESS THAN 2mm or 1/16 inch!!!
For liquid samples. dampen the tip of a toothpick and lightly dot.
Chamber Set-Up
Pour about 40 mL of rubbing
alcohol into the two 250 beakers (chamber)
Place aluminum foil over the beaker and use a rubber band to hold it in place
Leave chamber in the hood
Chromatography Paper Set Up
Draw a line 1/2 inch from bottom of paper
Make 2-3 small tick marks equally spaced apart
Underneath each tick mark, label them in PENCIL:
* Food Coloring, Pen, & Marker
* Unknown 1 & Unknown 2
Do so to each paper
Once paper is dry, fold it on top so that it stands alone.
Without science, there is no chemistry. Or hormones

1 Pen and 1 marker to analyze
Food coloring
Materials
Paper
Chromatography
TLC
Liquid Chromatography
Gas Chromatography
Unknown M&M mixture
1 worksheet
100 mL Rubbing alcohol
in a beaker
Rubber bands
2- 400 mL beakers
4- toothpicks
1-ruler
2 pieces of Chromatography paper

2 pieces of aluminum foil
Full transcript